Trichloro(N,N-dimethyloctylamine)boron

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

29 April 1996 to 3 May 1996

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: No GLP methodology followed.

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

no

Qualifier:

according to guideline

Guideline:

EU Method C.1 (Acute Toxicity for Fish)

Deviations:

no

GLP compliance:

no

Analytical monitoring:

no

Vehicle:

yes

Test organisms (species):

Cyprinus carpio

Details on test organisms:

- Species: Carp (Cyrinus carpio, Teleostei, cyprinidae) (Linnaeus, 1758)
- Source: Zodiac proeffac, "DeHaar Vissen", L.U. Wageningen, The Netherlands.
- Mean lenght: 2.4 +/- 0.25 cm
- Meam weight: 0.24 +/- 0.05 cm
- Characteristic: F1 from a single parent-pair bred in UV-treated water.
- Reason for selection: This system has been selected as an internationally accepted species.
- Quatantin/ Acclimation: At least 12 days after delivery
- Feeding: Daily with Trouvit

Validity of batch: In the batch of fish used for the test, mortality during the seven days prior to the start of the test was less than 5%.

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Post exposure observation period:

At 4, 24, 48, 72 and 96 hours following the start of exposure.

Hardness:

250 mg CaCO3 per liter

Test temperature:

20.5 to 20.6 °C

pH:

8.0

Dissolved oxygen:

Dissolved Oxygen (DO): 9.1 at day 0 and 8.4 at day 4.

Salinity:

Not applicable

Nominal and measured concentrations:

Nominal concentrations: 0.1/ 1.0/ 10 and 100 mg/l

Details on test conditions:

- Test vessels: 5 liters, all-glass
- Test medium: ISO-medium, aerated until the dissolved oxygen concentration had reached saturation and the pH had stabilized.
- Number of fish: 7 fish exposed to 100 mg/L and teh controls. 3 fish exposed to 0.1, 1.0, and 10 mg/l.
- Loading: 0.4-0.6 g fish/liter, i.e. 3 fish per 2 litres or 7 fish per 3 litres of test medium.
- Illumination: 16 hours photoperiod daily
- Aeration: The test media were not aerated during the test
- Feeding: No feeding from 24 hours prior to the test and during the total test period.
- Introduction of fish: Directly after preparation of the test media.
- Euthanasia: At the end of the test the surviving fish were rapidly killed by exposing them to ca. 1.2% ethylene glycol monophenylether in water.

Reference substance (positive control):

no

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Results with reference substance (positive control):

Not applicable

Sublethal observations / clinical signs:

solved oxygen values measured at the start and the end of the test. Temperature of the medium ranged from 20.5 to 20.6°C during the test.

Table1: Incidence and total mortality:

Concentration (mg/L)	Initial number of fish	Cumulative mortality				Total mortality (%)
		24 h	48 h	72 h	96 h	Total mortality (%)
Blanl-control	7	0	0	0	0	0
Solvent control	7	0	0	0	0	0
0.1	3	0	0	0	0	0
1.0	3	0	0	0	0	0
10	3	1	1	1	1	33
100	7	0*	0*	1*	1*	10

*Substance desposits were observed in this level.

 

Table 2: Incidence of clinical effects:

Concentration (mg/L)	Initial number of fish	Incidence of clinical effects				Total affected (%)
		24 h	48 h	72 h	96 h	Total affected (%)
Blank-control	7	0	0	0	0	0
Solvent-control	7	0	0	0	0	0
0.1	3	0	0	0	0	0
1.0	3	0	0	0	0	0
10	3	0	H=2/2	H=2/2	H=2/2	100
100	7	H=7/7	H=7/7	H=6/6	H=2/2	100

H: Number of fish that were hypoactive compared to the controls.

 

 

Table 3: pH and dissolved oxygen:

Concentration mg/L	Day 0 pH	Day 0 DO	Day 4 pH	Day 4 DO
Blank-control	8.1	9.1	7.9	8.4
Solvent-control	8.1	9.1	-	-
0.1	8.1	9.1	-	-
1.0	8.1	9.1	-	-
10	8.1	9.1	-	-
100	8.1	9.1	7.4	8.4

DO: Dissolved oxygen

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Validity criteria fulfilled:

yes

Conclusions:

Under the study conditions, the nominal 96h-LC50 was >100 mg/L.

Executive summary:

A study was conducted to determine the acute toxicity of the test substance, trichloro(N,N-dimethyloctylamine) boron, to Cyprinus carpio according to OECD Guideline 203 and EU Method C.1 without GLP. The definitive test was performed with carp exposed to concentrations ranging from 0.1 to 100 mg/L in a static system. Acetone was used as a solvent in the stock solution. The nominal 96 h LC50 was >100 mg/L. The maximum solubility was estimated to be between 10 and 100 mg/L. Hence, the testing of concentrations greater than 100 mg/L was not considered to be relevant. No analytical dose verification was conducted. Under the study conditions, the nominal 96 h LC50 was determined to be 100 mg/L (Bogers, 1996).

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Dose descriptor:

LC50

Effect concentration:

100 mg/L

Additional information

A study was conducted to determine the acute toxicity of the test substance, trichloro(N,N-dimethyloctylamine) boron, to Cyprinus carpio according to OECD Guideline 203 and EU Method C.1 without GLP. The definitive test was performed with carp exposed to concentrations ranging from 0.1 to 100 mg/L in a static system. Acetone was used as a solvent in the stock solution. The nominal 96 h LC50 was >100 mg/L. The maximum solubility was estimated to be between 10 and 100 mg/L. Hence, the testing of concentrations greater than 100 mg/L was not considered to be relevant. No analytical dose verification was conducted. Under the study conditions, the nominal 96 h LC50 was determined to be 100 mg/L (Bogers, 1996).