2-chloroacetamide

Short-term toxicity to aquatic invertebrates

Description of key information

48 hour EC50 Daphnia magna: 19.8 mg/L
96 hour LC50 Enchytraus albidus: 8.9 mg/L

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

8.9 mg/L

Additional information

 

In the key study from BASF, the acute toxicity of 2-chloroacetamide - which contains at least 98% acitve ingredient - to Daphnia magna was determined in a static GLP compliant test. The test was performed according to EEC C.2 and OECD 202 without deviations to the guidelines. Since the test was performed under GLP and the standard guidelines were followed and the report is well documented, the study is scored as Klimisch 1.

The test was started with a control and nominal 5.8, 10, 18, 32, 58 and 100 mg test item /L. Dose verification confirmed that the test solutions were correctly applied and that the test material was stable throughout the exposure period. Hence the obtained results can be based on nominal concentrations. Four replicates with 5 daphnids < 24 hours were set up at the start of the test.

After 48 hours of exposure, 0, 5, 0, 95, 100, 100 and 100 % of the daphnids were immobilized at 0 (control), nominal 5.8, 10, 18, 32, 58 and 100 mg test item /L, respectively. The corresponding 48 h EC50 value is 14 mg test item/L.

This study is considered to be acceptable for the risk assessment.

In the supporting study from Roembke et al (1989) they performed a comparison study with the enchytraeid E. albidus with the aim to compare the sensitivity of this organism with the sensitivity of Daphnia magna. It is not mentioned that the tests were performed under GLP. The study methods and the parameters for evaluation are clearly described. The test procedures followed in general the procedures outlined in the OECD 202 but the tests were extended to 96 hours. The results for Chloroacetamid were not provided in detail. Only the LC50 value was reported as 8.9 mg/L. For other chemicals more details on the results (dose response curves) were provided. Based on this shortcoming, this information on the toxicity of the test item on E. albidus is scored as Klimisch 2. The 96 -hour LC50 of E. albidus for Choroacetamid was determined as 8.9 mg/L.

In the paper from Roembke et al (1989), the toxicity to D. magna was cited as personal communication from Heimbach with an EC50 of 5.6 mg/L. Since no experimental details were provided this information from Heimbach is scored as Klimisch 4.

This study with E. albidus is considered to be acceptable for the risk assessment, the EC50 for Daphnia magna reported in the same paper will not be used..

The toxicity to daphnids as determined in the BASF study will be used for the classification and labelling. The toxicity to E albidus will be used for the risk assesment.