Registration Dossier

Administrative data

Endpoint:
toxicity to other aquatic vertebrates
Type of information:
experimental study
Adequacy of study:
other information
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Well documented publication

Data source

Reference
Reference Type:
publication
Title:
Comparative Developmental Toxicity of Acetylenic Alcohols on Embryos and Larvae of Xenopus laevis
Author:
Dawson D.A., Schultz T.W., Baker L.L., Wilke T.S.
Year:
1990
Bibliographic source:
ASTM Special Tech Publ. Vol. 1096, 1990, p. 267-277

Materials and methods

Test guideline
Qualifier:
no guideline followed
Principles of method if other than guideline:
Xenopus laevis embryos and larvae were tested to assess the relative toxicity and teratogenicity of a series of 14 aetyknic alcohols. Tertiary propargylic alcohols and alkene-ols were expected to act as simple narcotics and not be teratogenic. However, primary and secondary propargylic and homopropargylic alcohols might be metabolically activated, by alcohol dehydrogenase, to become teratogenic Mid-to-late blastula stage embryos were exposed to graded concentrations of each alcohol for 96 h. Embryo malformation (fmECw) and lethality (EmLCw) endpoints were determined for each alcohol, and the Mortality/Malformation Index (MMI) was calculated as a measure of relative tetatogenic potential. Additionally, untreated, healthy-appearing 5-day-old tadpoles were exposed to each alcohol for 96 h, and the tadpole lethality endpoint (TdLCm) was determined. The ratio of the embryo LCw to tadpole LCn (E/T) was calculated as a measure of aloohol reactivity. The primary propargylic alcohols had teratogenic potential, producing head, eye, gut, and skeletal malformations and MMI values > 3 .0. All other alcohols caused only edema and improper gut coiling.The E/T ratios indicated that primary and secondary propargylic and homopropargylic alcohols were reactive, whereas tertiary propargylic alcohols and alkene-ols were not. In addition, quantitative strucure-activity relationships (QSARs) were explored, and comparisons of tadpole and fathead minnow lethality data indicated that toxicity in one system can be used to predict toxicity in the other.
GLP compliance:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
Purity: >= 95 %

Test organisms

Test organisms (species):
Xenopus laevis

Study design

Total exposure duration:
96 h

Results and discussion

Effect concentrationsopen allclose all
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
5.41 mmol/L
Basis for effect:
mortality
Remarks on result:
other: Embryo mortality
Duration:
96 h
Dose descriptor:
EC50
Effect conc.:
1.55 mmol/L
Basis for effect:
other: Teratogenetic effects on Embryos
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
1.8 mmol/L
Basis for effect:
mortality
Remarks on result:
other: Tadpole mortality

Any other information on results incl. tables

The lowest effect value of 1.55 mmol/L for teratogenic effect on tadpole embryos was recalculated by use of the substances mol weight into an effect concentration of 192.48 mg/L

Applicant's summary and conclusion