Absolute of Cistus ladaniferus (Cistaceae) obtained from labdanum resinoid by ethanol extraction.

Density

Link to relevant study record(s)

Reference

Endpoint:

relative density

Type of information:

experimental study

Adequacy of study:

key study

Study period:

From 31 January 2018 to 02 February 2018

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Justification for type of information:

The study was conducted according to an internationally recognised method, and under GLP. The substance is considered to be adequately characterised. Therefore full validation applies.

Qualifier:

according to guideline

Guideline:

OECD Guideline 109 (Density of Liquids and Solids)

Version / remarks:

2012

Deviations:

no

Qualifier:

according to guideline

Guideline:

EU Method A.3 (Relative Density)

Version / remarks:

2008

Deviations:

no

Principles of method if other than guideline:

Not applicable

GLP compliance:

yes (incl. QA statement)

Remarks:

inspected on 15-16 November 2017 / signed on 15 May 2018

Type of method:

pycnometer method

Remarks:

with replacement liquid

Key result

Type:

density

Density:

1.045 g/cm³

Temp.:

20 °C

Remarks on result:

other:

Remarks:

mean of two independent determinations

Key result

Type:

relative density

Density:

1.045 other: unitless

Temp.:

20 °C

Remarks on result:

other:

Remarks:

mean of two independent determinations

Pre-test

At 50°C, the test item was slowly flowable. Then it was tested if 40°C were also sufficient. As the test item was also slowly flowable 40°C were considered as the lowest possible temperature at which it was flowable. After cooling down deionised water was added. The test item was not dissolved and the solution stayed clear. Therefore deionised water was considered as applicable for replacement liquid.

Table 4.4/1: Measured Values

 

First Determination				Error rel.	Error abs.
Temperature	19.8	°C			0.1
Density of water	0.998203	g/cm^3		2.00%	0.0002
Weighing #	m1	m2	m3	m4	m5
Item	Pycn. empty	Pycn. + H2O	Pycn. + repl.liquid	Pycn. + test item	Pycn. + test item + repl. liquid
Mass in g	38.0022	88.3889	88.3701	48.0440	88.8230
Temperature in °C	20.0	19.8	20.0	20.0	19.8
Volume of Pycnometer	50.4774	cm^3
Density of Replacement liquid	0.9978	g/cm^3		Error rel.	Error abs.
Density of Test Item	1.0450	g/cm^3		0.0209%	0.0002
Second Determination				Error rel.	Error abs.
Temperature	20.0	°C			0.1
Density of water	0.998203	g/cm^3		2.00%	0.0002
Weighing #	m1	m2	m3	m4	m5
Item	Pycn. empty	Pycn. + H2O	Pycn. + repl.liquid	Pycn. + test item	Pycn. + test item + repl. liquid
Mass in g	38.0028	88.3819	88.3796	49.7348	88.8988
Temperature in °C	20.2	20.2	20.2	20.0	20.2
Volume of Pycnometer	50.4698	cm^3
Density of Replacement liquid	0.9982	g/cm^3		Error rel.	Error abs.
Density of Test Item	1.0444	g/cm^3		0.0182%	0.0002

Results of the single determinations with the respective error as well as the mean value and its standard deviation are presented in the following tables:

Table 4.4/2: Results of Single Determinations

 

Determination	Density in g/cm^3	Error of Determ. in g/cm^3
1	1.0450	0.0002
2	1.0444	0.0002

 

Table 4.4/3 Results – Mean Values

 

Parameter	Value	Unit	Standard Deviation	Relative Standard Deviation
Volume of Pycnometer	50.47	cm^3	0.0054	0.01%
Density of Test Item	1.0447	g/ cm^3	0.0004	0.04%
Density of Test Item	1044.7	kg/m^3	0.4	0.04%
Relative Density of Test Item (D20/4)	1.0447		0.0004	0.04%

The density of the test item was calculated as 1.0447 ± 0.0002 g/cm³ at 20.0 ± 0.2 °C, which is the mean of two independent determinations ± the standard deviation. Standard deviation shows good correlation of the two determinations. Therefore, the study is considered valid.

Conclusions:

The density of the test substance was measured as 1.0447 ± 0.0002 g/cm3 at 20.0 ± 0.2 °C and the relative density is determined as D20/4 = 1.0447
Denser than water

Executive summary:

The density of the test substance was measured under GLP according OECD 109 / EU A3 guideline, using the pycnometer method.

Two determinations were performed and the mean value was retained.

This result was then converted to relative density.

Mean density: 1.0447 g/cm³at 20.0 ± 0.2 °C

Mean relative density (D20/4) : 1.0447

Description of key information

The density of the test item was measured as 1.0447 ± 0.0002 g/cm3 at 20.0 ± 0.2 °C and the relative density is determined as D20/4 = 1.0447

Key value for chemical safety assessment

Relative density at 20C:

1.045

Additional information

A fully reliable experimental study, conducted under GLP according to OECD 109 /EU A3 guideline, using pycnometer method with a replacement liquid, is available. It is considered as a key study, and the result is retained as key data.