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EC number: 280-084-5 | CAS number: 82985-35-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 08 December 2011 - 22 December 2011
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: This study has been performed according to OECD and/or EC guidelines and according to GLP principles.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.11 (Biodegradation: Activated Sludge Respiration Inhibition Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- ISO 8192 (Water quality - Test for inhibition of oxygen consumption by activated sludge for carbonaceous and ammonium oxidation)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- The batch of Bis(trimethoxysilylpropyl)amine tested was a clear colourless liquid with a purity of 96.9%.
The test substance was pipetted below the surface of the Milli-RO-water and stirred briefly to mix (5 minutes). Subsequently, synthetic sewage feed and sludge were added and the test was initiated applying a 3 hour contact time. Preferably, the solution of the test substance in water should be pH neutral. As discussed with the sponsor the pH of a test substance solution test was not adjusted to pH 7.5 ± 0.5. - Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- The sludge was coarsely sieved, washed and diluted with ISO-medium. A small amount of the sludge was weighed and dried overnight at ca. 105°C to determine the amount of suspended solids (3.0 g/l of sludge, as used for the test). The pH was 7.7 on the day of testing. The batch of sludge was used one day after collection; therefore 50 ml of synthetic sewage feed was added per litre of activated sludge at the end of the collection day. The sludge was kept aerated at test temperature until use.
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Post exposure observation period:
- After the 3-hour contact time, the oxygen consumption was recorded for a period of approximately 10 minutes. During measurement, the sample was not aerated but continuously stirred on a magnetic stirrer.
The pH and temperature were determined in the remaining part of the reaction mixture. This procedure was repeated for all test/reference substance concentrations/loading rates and controls. - Test temperature:
- Final test: between 18.1 and 22.3°C
- pH:
- Final test
Controls, before addition of sludge: between the pH was between 7.4 and 7.6.
Controls, after the 3 hour exposure periode the pH was between 7.9 and 8.2.
Test concentrations, before addition of sludge: between the pH was between 7.7 and 9.1.
Test concentrations, after the 3 hour exposure periode the pH was between 7.9 and 8.4.
Discussion:
Bis(trimethoxysilylpropyl)amine has a slight increasing effect on pH (up to 9.1).
In general, lower (<5) and higher pH values (> approx. 8.5) might increase the toxic effect on micro-organisms. Thus the inhibition of the respiration rate may be caused by the intrinsic toxicity of Bis(trimethoxysilylpropyl)amine and/or the effect of the pH.
Thus, testing as is described in this report includes both effects and is thus a worse case situation. - Dissolved oxygen:
- The oxygen concentration at the start of measurement was at least 8.2 mg O2/l.
Oxygen consumption=Respiration rate (mg O2/l h):
Controls: 50 mg O2/l h
Bis(trimethoxysilylpropyl)amine at 10 mg/l: 54 mg O2/l h
Bis(trimethoxysilylpropyl)amine at 22 mg/l: 52 mg O2/l h
Bis(trimethoxysilylpropyl)amine at 46 mg/l: 56 mg O2/l h
Bis(trimethoxysilylpropyl)amine at 100 mg/l: 54 mg O2/l h
Bis(trimethoxysilylpropyl)amine at 220 mg/l: 45 mg O2/l h
Bis(trimethoxysilylpropyl)amine at 460 mg/l: 36 mg O2/l h
Bis(trimethoxysilylpropyl)amine at 1000 mg/l: 27 mg O2/l h - Nominal and measured concentrations:
- Nominal loading rates: 10, 22, 46, 100, 200, 460 and 1000 mg/l.
- Details on test conditions:
- TEST SYSTEM
- Type (delete if not applicable): open
- Material, size, headspace, fill volume: All glass
- Aeration:During exposure with clean, oil-free air
- No. of vessels per concentration (replicates):5
- No. of vessels per control (replicates): 6
- Biomass loading rate 1.5 g/l suspended solids in final test mixture
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Tap-water purified by reverse osmosis (Milli-RO
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : The respiration rate from each vessel, in mg O2/l/hr, was calculated from the linear part of the respiration curve, which was generally between 2 and 7 mg O2/l.
TEST CONCENTRATIONS
- Test concentrations: Seven loading rates: 10, 22, 46, 100, 200, 460 and 1000 mg/l. - Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol
- Duration:
- 3 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 220 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- loading rate
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Duration:
- 3 h
- Dose descriptor:
- EC10
- Effect conc.:
- 270 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- loading rate
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Duration:
- 3 h
- Dose descriptor:
- other: EC20
- Effect conc.:
- 420 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- loading rate
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- ca. 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- loading rate
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Remarks on result:
- other: estimated value
- Duration:
- 3 h
- Dose descriptor:
- other: EC80
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- loading rate
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Details on results:
- No statistically significant inhibition of the respiration rate of the sludge was recorded at or below a loading rate of 220 mg Bis(trimethoxysilylpropyl)amine / litre (Bonferroni t-Test: a=0.05 Toxstat). At higher loading rates the inhibitory effect of Bis(trimethoxysilylpropyl)amine on aerobic waste water (activated sludge) bacteria increased with increasing loading rate, ranging from 27% inhibition at a loading rate of 460 mg/l to 46% at a loading rate of 1000 mg/l.
- Results with reference substance (positive control):
- The EC50 of 3,5-dichlorophenol was in the accepted range of 2 to 25 mg/l for total respiration (8.2 in the combined limit-range-finding test and 8.1 mg/l in the final test).
- Reported statistics and error estimates:
- ECx
For Bis(trimethoxysilylpropyl)amine the percentage inhibition was plotted against the logarithm of the loading rates. The EC10 and EC20, for the test substance, were determined using linear regression analysis. If available, the 95% confidence limits of the EC values were given. The EC50 and EC80, were estimated.
For the reference substance the percentage inhibition was plotted against the logarithm of the concentrations and the EC50 was determined using linear regression analysis.
NOEC estimation
The NOEC was based on statistical analysis of the data. Data obtained for the test concentrations were compared with those obtained for the control using TOXSTAT Release 3.5, 1996, D.D. Gulley, A.M. Boelter, H.L. Bergman. - Validity criteria fulfilled:
- yes
- Conclusions:
- Under the conditions of this present test Bis(trimethoxysilylpropyl)amine was not toxic to waste water (activated sludge) bacteria at or below a loading rate of 220 mg/l (NOEC).
The EC10 was at a loading rate of 270 mg/l.
The EC20 was at a loading rate of 420 mg/l.
The EC50 was estimated at a loading rate of approximately 1000 mg/l.
The EC80 was above a loading rate of 1000 mg/l.
Reference
Description of key information
EC10 (3 h)= 270 mg/L and EC50 (3 h) = 1000 mg/L (nominal, OECD 209)
Key value for chemical safety assessment
- EC50 for microorganisms:
- 1 000 mg/L
- EC10 or NOEC for microorganisms:
- 270 mg/L
Additional information
The influence of the test item bis(trimethoxysilylpropyl)amine (CAS 82985 -35 -1) on the respiration rate of activated sludge was investigated according to the OECD guideline 209 and GLP (2012). The nominal test concentrations were 10, 22, 46, 100, 200, 460 and 1000 mg/L. No inhibition in respiration rate of the sludge was recorded up to 220 mg/L. Hence, the EC10 (3 h) for Bis(trimethoxysilylpropyl)amine (CAS 82985 -35 -1) is 270 mg/L. The respective EC50 (3 h) is 1000 mg/L.
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