cyclohexyl salicylate

Toxicity to soil macroorganisms except arthropods

Administrative data

Endpoint:

toxicity to soil macroorganisms except arthropods: short-term

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1993

Reliability:

2 (reliable with restrictions)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1993

Report date:

1993

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 207 (Earthworm, Acute Toxicity Tests)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Cyclohexyl Salicylate

IUPAC Name:

Cyclohexyl Salicylate

Details on test material:

Cyclohexyl Salicylate Lot No. SAT 921176/1096, Purity 98%

Sampling and analysis

Analytical monitoring:

no

Test substrate

Vehicle:

no

Test organisms

Test organisms (species):

Eisenia fetida

Animal group:

annelids

Study design

Study type:

laboratory study

Substrate type:

natural soil

Limit test:

no

Total exposure duration:

14 d

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

14 d

Dose descriptor:

LC50

Effect conc.:

> 560 - < 1 000 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Effect concentrations 2

Duration:

14 d

Dose descriptor:

LC0

Effect conc.:

560 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Any other information on results incl. tables

No Earthworm mortality was observed at concentrations up to and including 560 mg test substance/kg dry weight soil. Total mortality at the 1000 mg/kg dry weight soil test concentration was 60% on Day 7, and, remained at this level til the end of the study. The LC50 is situated between 560 and 1000 mg test substance/kg dry weight soil. The LC0 is 560 mg test substance/kg dry weight soil. No significant weight loss was observed up to and including 560 mg test substance/kg dry weight soil test concentration. Chloracetamide demonstrated an LC0 and LC100 of, respectively, 10 and 100 mg Chloracetamide/kg dry weight soil.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Executive summary:

The toxicity of CYCLOHEXYL SALICYLATE to the earthwormEisenia fetidaandrei over an exposure period of 14 Days. Following a range.finder test, the definitive test was set-up with 5 test concentrations (94, 170, 310, 560 and 1000 mg test substance/kg dry weight soil) and a Control. Soil was maintained in 2 L containers with air-flow through covers. Adult earthworms of at least 3 Months old were introduced in to the soil. Samples were examined for mortality and other observable effects (mucous secretion, weariness, loss of weight) on Day 7 and Day 14. Soil moisture was 25.6% (prior to test substance addition) at the beginning and 26.4% at the end of the study. Soil pH was 6.5, and the incubation temperature ranged from 21.5 °C to 22.0 °C. Chloracetamide was employed as the toxicity reference substance.