cyclohexyl salicylate

Bioaccumulation: aquatic / sediment

Administrative data

Endpoint:

bioaccumulation in aquatic species: fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1993

Reliability:

2 (reliable with restrictions)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1993

Report date:

1993

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 305 E (Bioaccumulation: Flow-through Fish Test)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

-

EC Number:

400-410-3

EC Name:

-

Cas Number:

25485-88-5

Molecular formula:

C13H16O3

IUPAC Name:

cyclohexyl 2-hydroxybenzoate

Details on test material:

Carboxyl labelled 14C, Batch No. 92301, specific activity 9.41 MBq/mg, dissolved in Ethanol with Specific Activity 16.7 MBq/mL, Radiopurity > 98%.

Radiolabelling:

yes

Test solutions

Vehicle:

yes

Test organisms

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Study design

Route of exposure:

aqueous

Test type:

flow-through

Water / sediment media type:

natural water: freshwater

Total exposure / uptake duration:

28 d

Total depuration duration:

14 d

Test conditions

Nominal and measured concentrations:

At 1 ug/L test concentration, the actual measured concentration was determined to be 1.05 ug/L.
At 10 ug/L test concentration, the actual measured concentration was determined to be 8.5 ug/L.

Results and discussion

Bioaccumulation factor

Bioaccumulation factor 1

Type:

BCF

Value:

600 - 900 L/kg

Basis:

whole body w.w.

Time of plateau:

2 d

Calculation basis:

steady state

Remarks on result:

other: Conc.in environment / dose:1microg/L

Bioaccumulation factor 2

Type:

BCF

Value:

600 - 900 L/kg

Basis:

whole body w.w.

Time of plateau:

2 d

Calculation basis:

steady state

Remarks on result:

other: Conc.in environment / dose:10microg/L

Depuration

Depuration 1

Elimination:

yes

Parameter:

DT50

Depuration time (DT):

3 d

Depuration 2

Parameter:

DT90

Depuration time (DT):

5 d

Any other information on results incl. tables

1mg/L exposure level– Actual measured concentration was 1.05mg/L. At least half- to ¾ of the total radioactivity recovered via extraction was in the form of the Parent material. The BCF based on CYCLOHEXYL SALICYLATE was calculated to be between 600 and 900 L/kg (wet wt.). Steady state was attained within the first 2 Days of exposure. > 50% of the accumulated radioactivity had dissipated within the first 2- to 3-days of the depuration phase (Ct50 < 3 days and Ct90 < 5 days).10mg/L exposure level- Actual measured concentration was 8.5mg/L. At least half- to ¾ of the total radioactivity recovered via extraction was in the form of the Parent material. The BCF based on CYCLOHEXYL SALICYLATE was calculated to be between 600 and 900 L/kg (wet wt.). Steady state was attained within the first 2 Days of exposure. > 50% of the accumulated radioactivity had dissipated within the first 2- to 3-days of the depuration phase (Ct50 < 3 days) . 

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Executive summary:

The potential of CYCLOHEXYL SALICYLATE to bioaccumulate in fish was studied using C-14 (carboxyl) labelled test substance at two test concentrations using Zebrafish (Brachydanio rerio). The fish were acclimated in drinking water for at least 2 Weeks at a temperature of 23 °C, and the validity of the fish stock verified. Prior to the start of the exposure phase, lipid content was determined from 15 fish.

Uptake Phase– The study was conducted as a flow-through test at 23 °C in filtered drinking water. Fish were exposed in 6 Litre tanks. Ten volume exchanges were performed per day. Oxygen concentration was > 60% of saturation. Two exposure concentrations were performed : 1mg/L and 10mg/L. Fish samples were taken at regular intervals (Day0, 2, 6, 12, 20 and 28), left to drip dry, euthanised and combusted to determine total C-14 content via¹⁴CO₂. At the end of the uptake phase (Day 28), 15 fish were sampled from the 1mg/L and extracted with ethanol to determine test material. There was a > 20% variation in the test material concentration in the test vessels during the first 2 Days of the uptake phase presumably due to strong adsorption of the test material.

Depuration Phase– Fish were transferred from the treated aqueous phase to clean water containing no test substance, and, incubated for a period of 14 Days. Fish were removed for analysis on Day 30, 33, 37 and 42. The lipid content was determined on fish from the 10mg/L treatment level at the end of the depuration phase of the study.