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EC number: 202-784-1 | CAS number: 99-75-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Study period:
- 1988
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: well performed OECD study but without GLP
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 988
- Report date:
- 1988
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Deviations:
- yes
- Remarks:
- only 4 instead of 5 concentrations, industrial insted of domestic sludge
- GLP compliance:
- not specified
Test material
- Reference substance name:
- Methyl p-toluate
- EC Number:
- 202-784-1
- EC Name:
- Methyl p-toluate
- Cas Number:
- 99-75-2
- Molecular formula:
- C9H10O2
- IUPAC Name:
- methyl 4-methylbenzoate
- Details on test material:
- purity: > 99.0 %
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Vehicle:
- no
Test organisms
- Test organisms (species):
- activated sludge, industrial
- Details on inoculum:
- Origin: Sewage treatment plant of the Hoechst AG, site Kelsterbach
Type of inoculum: Industrial activated sewage sludge
Preparation of the suspension: sediment from activated sewage sludge was washed three times by centrifuging and resuspending in water with 0.9 % NaCl
Content of suspended solids in the sewage sludge suspension : normally 2 g/L (dry matter) were used
Pre-treatment: corresponding to the cited test guideline, but no detail was recorded in the laboratory test protocol
State of acclimatization : not pre-adapted in the laboratory to the test substance or the reference substance
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Post exposure observation period:
- no
Test conditions
- Test temperature:
- 22.7 - 23.1 °C
- pH:
- 8.9
- Nominal and measured concentrations:
- Nominal:
Concentration of the stock solution . : 380 mg DOC/L (analytical value)
According to : 528 mg/L of the substance (calculated from DOC)
Addition of the stock solution : Dilutions 1:2; 1:4, 1:8, and 1:16
Test substance in the batches: 264, 132, 66, and 33 mg/L
Stock solution:
Addition of the test substance: 2000 mg in 1000 ml of de-ionized water
The batch was stirred over 24 hours in a covered beaker at a temperature of about 21°C
The substance sample weighed in was only partly dissolved. The batch was passed through a folded filter. The filtrate was used as stock solution.
The concentration was measured as dissolved carbon content (DOC). - Details on test conditions:
- Incubation vessels : Glasbottles with a diameter of 60 mm and a grounded neck (NS 29), height 170 mm and a total volume of approx. 480 ml. The bottles contained magnetic stirrer bars and were incubated in a water bath on magnetic stirrers.
Test volume : 200 ml each batch
Temperature control: laboratory thermostat
Aeration rate/test vessel: normally 45 litres per hour, but the value is not documented in the laboratory manuscript; aeration only during the incubation period
Oxygen measurements : With oxygen electrodes, connected to oximeters and two-channel recorders
Monitoring : During the measuring periods of about 10 minutes without further aeration the oxygen concentration curves were plotted by the recorders
Respiration rate : The slope of the linear part of the respiration curves accorded to the corresponding respiration rates
Measurements of the pH: pH Meters - Reference substance (positive control):
- no
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 3 h
- Dose descriptor:
- other: 0 % respiration inhibition
- Effect conc.:
- 264 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- dissolved
- Basis for effect:
- other: respiration inhibition
- Duration:
- 3 h
- Dose descriptor:
- other: 6% respiration inhibition
- Effect conc.:
- 132 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- dissolved
- Basis for effect:
- other: respiration inhibition
- Duration:
- 3 h
- Dose descriptor:
- other: 0% respiration inhibition
- Effect conc.:
- 66 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- dissolved
- Basis for effect:
- other: respiration inhibition
- Duration:
- 3 h
- Dose descriptor:
- other: 0% respiration inhibition
- Effect conc.:
- 33 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- dissolved
- Basis for effect:
- other: respiration inhibition
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The EC0 and the EC50 of the substance to industrial activated sludge (Sewage treatment plant of the Hoechst AG, site Kelsterbach) were in the range >100 mg/L to 1000 mg/L.
- Executive summary:
The substance was tested for toxicity to bacteria according to OECD guideline 209 in the Respiration Inhibition Test with activated sludge from an industrial waste water treatment plant. The EC0 and EC50 values were found to be in the range 100 - 1000 mg/L.
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