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EC number: 844-009-5 | CAS number: 167173-85-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to soil microorganisms
Administrative data
- Endpoint:
- toxicity to soil microorganisms
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 012
- Report date:
- 2012
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- other: SETAC Guideline - Procedures for Assessing the Environmental Fate and Ecotoxicity of Pesticides, Soil Micro-organisms
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 216 (Soil Microorganisms: Nitrogen Transformation Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 217 (Soil Microorganisms: Carbon Transformation Test)
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- (3S,6S,7R,8R)-8-benzyl-3-(3-hydroxy-4-methoxypyridine-2-amido)-6-methyl-4,9-dioxo-1,5-dioxonan-7-yl 2-methylpropanoate
- Cas Number:
- 167173-85-5
- Molecular formula:
- C26H30N2O9
- IUPAC Name:
- (3S,6S,7R,8R)-8-benzyl-3-(3-hydroxy-4-methoxypyridine-2-amido)-6-methyl-4,9-dioxo-1,5-dioxonan-7-yl 2-methylpropanoate
Constituent 1
- Specific details on test material used for the study:
- Substance name: X642188 (metabolite of XDE-777)
Lot#: YC2-106153-15
Purity: 99%
Sampling and analysis
- Analytical monitoring:
- no
Test substrate
- Vehicle:
- yes
Test organisms
- Test organisms (inoculum):
- soil
Study design
- Total exposure duration:
- 28 d
Test conditions
- Test temperature:
- 18-22°C
- Moisture:
- 46% - 49%
- Organic carbon content (% dry weight):
- 0.78
- Nominal and measured concentrations:
- 0.088 and 0.438 mg/kg soil dw
- Reference substance (positive control):
- yes
- Remarks:
- Sodium chloride was applied at a rate of 16 g/kg dry soil in a separate study within one year of the start of the experimental phase of this study
Results and discussion
Effect concentrationsopen allclose all
- Key result
- Duration:
- 28 d
- Basis for effect:
- nitrate formation rate
- Remarks on result:
- other: The difference in the soil nitrate formation rates between the control and both treatments was clearly below the OECD guideline 216 trigger value of 25 % at the 14 to 28 day interval. These deviations were not statistically significant different
- Key result
- Duration:
- 28 d
- Dose descriptor:
- other:
- Basis for effect:
- respiration rate
- Remarks on result:
- other: The soil respiration rates were within the trigger value of ±25% set by OECD guideline 217 throughout the experiment. On day 28 the values differed by 2.43% and 1.10% from the control for the low and high dose rate, respectively.
- Remarks:
- There were statistically no significant differences on day 28 between control and both test item treated groups.
Applicant's summary and conclusion
- Conclusions:
- Based on the results of this study, it is concluded that the test substance had no significant impact on soil microorganisms (carbon mineralisation and nitrogen transformation) when applied at concentrations up to 0.438 mg/kg soil dry weight. It can be concluded that the test substance will not have any long term influence on soil microorganisms.
- Executive summary:
The objective of the study was to evaluate the effects of the test substance on the activity of soil microflora in the laboratory. The study was conducted according to OECD Guidelines 217 and 216.
Biologically active agricultural soil (mid loamy sand) was used. Control, 0.088 and 0.438 mg/kg soil dry weight are the test rates used in this study. Endpoints observed were O2-consumption and NO3-nitrogen production after 28 days of exposure
Soil respiration was determined in soil after addition of glucose. Three replicates were used per treatment and concentration. A BSB-Sensomat System® was used to determine the CO2-production over a period of up to 24 hours at different sampling intervals.
Nitrogen-transformation (nitrate-nitrogen levels) was determined in soil enriched with lucerne mean (concentration in soil 0.5%). Three replicates were used per treatment and concentration. NO3-nitrogen formed from the nitrification process was determined by means of a Dionex ion chromatography system.
Sampling was done 0, 7, 14 and 28 days after treatment (soil respiration and soil nitrogen transformation test). Sub-samples were withdrawn from the soil bulk batches and subjected to analysis.
Soil moisture was 46% to 49% of its maximum water holding capacity (carbon and nitrogen transformation). Soil samples were incubated at 20 °C ± 2 °C while stored in plastic boxes covered by perforated lids. The soil pH was in the range of 7.0 to 7.2.
Calculation of mean values per treatment, standard deviation and coefficient of variation. Normality and homogeneity of variances were tested using the R/S-Test Test (α = 0.05) and Levene´s test (α = 0.05), respectively, and pair-wise comparisons of treated and control values according to Student t-test (α = 0.05) were performed.
The soil respiration rates were within the trigger value of ±25% set by OECD guideline 217 throughout the experiment. On day 28 the values differed by 2.43% and 1.10% from the control for the low and high dose rate, respectively. There were statistically no significant differences on day 28 between control and both test item treated groups (Welch t-test, α = 0.05).
No adverse effects of test substance on nitrogen transformation in soil could be observed in both test substance concentrations (0.088 mg/kg dry soil and 0.438 mg/kg dry soil) after 28 days. Deviations from the control of 4.21% (application rate 0.088 mg/kg dry soil) and 8.39% (application rate 0.438 mg/kg dry soil) were measured at the end of the 28-day incubation period. There was a statistically significant difference on day 28 between control and the high treatment group (Student t-test, α = 0.05).
The soil nitrate formation rates were calculated on an incremental basis (i.e. between successive sampling dates). In the last interval between days 14 and 28, the deviations from control were -3.92% and 2.61% for the low and high test concentration of test substance. Thus, the difference in the soil nitrate formation rates between the control and both test item treatments was clearly below the OECD guideline 216 trigger value of 25 % at the 14 to 28 day interval. These deviations were not statistically significant different (Student t-test, α = 0 .05).
Based on the results of this study, it is concluded that the test substance had no significant impact on soil microorganisms (carbon mineralisation and nitrogen transformation) when applied at concentrations up to 0.438 mg/kg soil dry weight.
It can be concluded that the test substance will not have any long term influence on soil microorganisms.
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