Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 858-735-5 | CAS number: 2337348-25-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2019-09-03 to 2019-10-01
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
- Version / remarks:
- 1992-07-17
- Deviations:
- no
- Principles of method if other than guideline:
- 'Standard Concerning Testing Facility Relating to New Chemical Substances' of Japanese Chemical Substances Control Law (March 31, 2011, Yakushokuhatsu 0331 No. 8, Heisei 23.03.29 Seikyoku No. 6; Kampokihatsu No. 110331010)
- GLP compliance:
- yes (incl. QA statement)
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge (adaptation not specified)
- Details on inoculum:
- - Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): Activated sludge was obtained from an aeration tank of a sewage treatment plant treating predominantly domestic sewage in Kurume-shi, Fukuoka, Japan.
- Method of cultivation: The activated sludge was aerated at approximately 22 °C until use for the test.
- Storage conditions:
- Storage length: 2019-09-02
- Preparation of inoculum for exposure: Additive amount of the activated sludge into the test vessel was 4.05 mL on the basis of the concentration of suspended solid in the activated sludge which was determined by the following method: Japanese Industrial Standards (JIS) K 0102:2016 Section 14.1.
- Concentration of sludge: 30 mg/L (as concentration of suspended solid)
- Water filtered: no - Duration of test (contact time):
- 28 d
- Initial conc.:
- 100 mg/L
- Based on:
- not specified
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- TEST CONDITIONS
- Composition of medium: The mineral medium (4 L) was prepared at the same proportion as the following method: 10 mL of solution A and each 1 mL of solutions B, C, and D prescribed in the test methods in Section 5. were made up to 1 L with purified water (The Japanese Pharmacopoeia, Takasugi Pharmaceutical).
- Test temperature: 22 ± 1
- pH: 7.4
- pH adjusted: yes
- Aeration of dilution water: yes
- Continuous darkness: yes
TEST SYSTEM
- Culturing apparatus: Closed system consumption measuring apparatus (No. CM-058) Temperature controlled bath with measuring unit OM3100A (Ohkura Electric)
- Number of culture flasks/concentration: 1-2 (see below)
Test solution (sludge + test item): 2 replicate
Procedure control (sludge + sodium benzoate): 1 replicate
- Method used to create aerobic conditions: Aeration
- Measuring equipment: Soda lime No. 1 (for absorption of carbon dioxide, FUJIFILM Wako Pure Chemical)
- Test performed in closed vessels: yes
SAMPLING
- Sampling frequency: BOD continuously, analytical samples at end of incubation period
- Sampling method:
BOD: closed system oxygen consumption apparatus, TOC analyzer
Chemical analysis: HPLC & LC-MS
CONTROL AND BLANK SYSTEM
- Inoculum blank (test solution; control blank): 2 replicates
- Toxicity control (sludge + test item + sodium benzoate): 1 replicate
STATISTICAL METHODS:
The percentage biodegradation was rounded of to the whole number.
In the calculation of TOD of the test item, the compositional formula C18H33O5 obtained by elemental analysis of the test sample was used.
The TOD of the test solution (sludge + test item + sodium benzoate) was the sum of TOD of the test item and that of sodium benzoate.
In addition, the percentage biodegradation by BOD of the test item at the end of the 10-day window (the 10 days immediately following the attainment of 10% biodegradation) was calculated.
The atomic weight of each element used in this study was based on the 4-digit atomic weight table 2017 provided by the Chemical Society of Japan. - Reference substance:
- benzoic acid, sodium salt
- Key result
- Parameter:
- % degradation (test mat. analysis)
- Remarks:
- by HPLC
- Value:
- 100
- Sampling time:
- 28 d
- Key result
- Parameter:
- % degradation (DOC removal)
- Value:
- >= 87 - <= 90
- Sampling time:
- 28 d
- Results with reference substance:
- Criteria: Percentage degradation of the reference compound has reached the pass levels by day 14 (60%):
Yes, 89% measured. - BOD: 3 %
- DOC: 3 %
- Measured values: 23 and 25 mg/L
- Measured values: 7.3-7.4
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- inherently biodegradable
- Conclusions:
- The test item disappeared completely and two degradation products were produced under the test conditions of the study. The degradation products were intermediates in the process of biodegradation and they will be completely biodegraded eventually. The test item was considered to have inherent biodegradability.
Reference
Validity criteria
Difference between extreme of replicate values of percentage biodegradation in test solutions (sludge + test item) on Day 28 should not be greater than 20 %:
BOD in test solutions (control blank) on Day 28 should not be greater than 60 mg/L:
pH values should be in a range from 6-8.5:
Biodegradability of test item
The averages of the percentage biodegradation were 69% by BOD and 89% by DOC on Day 28. In addition, growth of the sludge was observed at the end of the incubation. These results showed that most of the test item underwent biodegradation under the test conditions of the study.
According to OECD test guideline 301F, the pass level for biodegradability is 60% biodegradation by BOD on Day 28 and in the 10-day window. In the study, the percentage biodegradation by BOD of the test item at the end of the 10-day window was 49% and 54%. These values did not reach the pass level. However, since both of the percentage biodegradation by BOD were 67% and 70% and the BOD cuves were rising on Day 28 in the test solutions (sludge + test item), the test item was considered to have inherent biodegradability.
Quantitative analysis of test item and qualitative analysis of degradation products
The percentage residue of the test was 0% and a peak corresponding to the degradation product (D2) was 13% and 10%. These results showed that water-soluble degradation products were produced. As a result of the qualitative analysis by LC-MS, two degradation products (D1 and D2) were detected on the TIC chromatogram. Since the degradation products were water-soluble, it was presumed that the percentage production of the degradation products was equal to the percentage residue of DOC (13% and 10%). The averages of the percentage biodegradation were 69% by BOD and 89% by DOC on Day 28, and the BOD curves were rising on Day 28 in the test solutions (sludge + test item). That is, the degradation products were intermediates in the process of biodegradation and they will completely biodegrade eventually.
Description of key information
The test item disappeared completely and two degradation products were produced under the test conditions of the study. The degradation products were intermediates in the process of biodegradation and they will be completely biodegraded eventually. The test item was considered to have inherent biodegradability.
Key value for chemical safety assessment
- Biodegradation in water:
- inherently biodegradable
- Type of water:
- freshwater
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.