Registration Dossier
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EC number: 292-124-9 | CAS number: 90552-04-8
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
Description of key information
ErL10 (72h): >100 mg/L
NOErL (72h): >=100 mg/L
Key value for chemical safety assessment
Additional information
In a 72-hour algal growth study, cultures of Raphidocelis subcapitata were exposed to 2-Propenoic acid, 2-methyl-, C13-15-branched and linear alkyl esters at loading rates of 0 (control), and 100 mg/L under static conditions in accordance with the OECD 201 guideline. The water pH(a), temperature and lighting intensity were all maintained within acceptable guideline specifications.
The following effect concentrations (mg/L) were obtained after 72-h based on loading rate:
| Algal Yield [mg/L] | Algal Growth Rate [mg/L] | ||
EyL10 confidence limits 95%: | >100 - | ErL10 confidence limits 95%: | >100 - |
EyL20 confidence limits 95%: | >100 - | ErL20 confidence limits 95%: | >100 - |
EyL50 confidence limits 95%: | >100 - | ErL50 confidence limits 95%: | >100 - |
NOEyL | >=100 | NOErL | >=100
|
LOEyL | >100
| LOErL | >100 |
The test organisms were exposed to saturated solutions of the test substance in test media prepared at nominal loading rates of up to 100 mg/L in this study. The exposure was started after separation of the undissolved test substance.
The test substance had no observable inhibitory effect on Raphidocelis subcapitata growth up to its saturation limit in test media and under test conditions. The test substance is a mixture that is poorly soluble in water. Therefore the test solutions were prepared following general guidance provided in OECD 23 in order to achieve a water accommodated fractions (WAFs) of the test substance. The aqueous fraction of the test solution, after separation of the undissolved material, is a WAF and is considered analogous to nominal concentration (OECD 23).
The concentration of test substance in test media could not be verified analytically, therefore the effect concentrations are based on the loading rate used to prepare the test substance WAF solutions. According to guidance in OECD 23, for tests with chemicals that cannot be quantified by analytical methods at the concentrations causing effects, the effect concentration can be expressed based on the nominal concentrations or in the case of water accommodated fractions on the loading rate. The results in this study are consistent with all validity criteria and the test is valid according to the guidelines of this study.
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