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EC number: 605-140-1 | CAS number: 158237-07-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 16 Dec 1998 - 3 Jan 1999
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study without detailed documentation
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-E (Determination of the "Ready" Biodegradability - Closed Bottle Test)
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)
- GLP compliance:
- yes
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge: Secondary effluent of a domestic sewage treatment plant (Odenthal).
- Preparation of inoculum for exposure: Aeration for 5 days.
- Pretreatment: Separation of coarse particles by filtration.
- Initial cell/biomass concentration: 5 mL/L - Duration of test (contact time):
- 28 d
- Initial conc.:
- 4.1 mg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- TEST CONDITIONS
- Composition of medium: mineral medium
- Test temperature: 20 ± 1 °C
TEST SYSTEM
- Number of culture flasks/concentration: 2
SAMPLING
- Sampling frequency: Dissolved oxygen given for days 0, 7, 14, 21 and 28
CONTROL AND BLANK SYSTEM
- Inoculum blank: 2 replicates
- Reference control: 2 replicates
- Toxicity control: 2 replicates (4.1 mg/L test item, 2 mg/L reference substance) - Reference substance:
- aniline
- Remarks:
- 2 mg/L
- Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- 0
- Sampling time:
- 28 d
- Results with reference substance:
- In preparations with the reference substance aniline a degradation of 84 % was achieved within 14 days.
- Validity criteria fulfilled:
- yes
- Remarks:
- For further details please refer to “Any other information on results incl. tables”.
- Interpretation of results:
- not readily biodegradable
Reference
Table 1: Mean Dissolved Oxygen.
Sampling Time Points [d] | Inoculum blank | Reference Substance | Test substance | Toxicity control |
0 | 9.65 | 9.64 | 9.63 | 9.63 |
7 | 9.31 | 6.28 | 9.31 | 6.15 |
14 | 9.24 | 5.17 | 9.28 | 4.97 |
21 | 9.00 | 4.56 | 8.99 | 4.33 |
28 | 8.98 | 4.43 | 9.03 | 4.35 |
Table 2: % Degradation.
Sampling Time Points [d] | Reference Substance | Test substance | Toxicity control |
7 | 63 | 0 | 29 |
14 | 84 | 0 | 39 |
21 | 92 | 0 | 43 |
28 | 95 | 0 | 43 |
Table 3: Validity criteria for OECD 301 D.
Criterion from the guideline |
Outcome |
Validity criterion fulfilled |
Difference of extremes of replicate values of the removal of the test chemical at the plateau, at the end of the test or at the end of the 10-d window, as appropriate, is less than 20%. |
0% |
yes |
Percentage degradation of the reference compound has reached the pass levels by day 14. |
84% at Day 14 |
yes |
The toxicity control should degrade to at least 35% (based on DOC) or at least 25% (based on ThOD or ThCO2) within 14 d. |
39% at Day 14 |
yes |
Oxygen depletion in the inoculum blank should not exceed 1.5 mg dissolved oxygen/L after 28 days. |
0.67 mg O2/L |
yes |
The residual concentration of oxygen in the test bottles should not fall below 0.5 mg/L at any time. |
Did not fall below 0.5 mg/L. |
yes |
Description of key information
Not readily biodegradable: 0% biodegradation after 28 d (EU Method C.4-E).
Key value for chemical safety assessment
- Biodegradation in water:
- under test conditions no biodegradation observed
- Type of water:
- freshwater
Additional information
One study is available testing the biodegradability of the test substance by a non-adapted domestic activated sludge (1999). The test follows the principles of the EU Method C.4-E. and was conducted under GLP standards. 4.1 mg/L initial test item concentration was suspended in mineral medium and inoculated with the mixed population of activated sludge microorganisms. The mixture was incubated for 28 d and the biodegradation of the test material was determined by measuring the reduction of the dissolved oxygen. 0% degradation was determined at test termination revealing that the substance is not readily biodegradable. The toxicity control run in parallel showed that the substance is not inhibiting the sludge microorganisms (39% degradation of reference substance after 28 d).
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