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EC number: 269-284-3 | CAS number: 68214-04-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Hydrolysis
Administrative data
Link to relevant study record(s)
- Endpoint:
- hydrolysis
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- March 2018
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study without detailed documentation
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 111 (Hydrolysis as a Function of pH)
- Principles of method if other than guideline:
- Only a pre-liminary study is performed. In this study the degradation products were identified.
- GLP compliance:
- no
- Remarks:
- this internal study was used as verification the Laus study and followed the same protocol
- Radiolabelling:
- no
- Analytical monitoring:
- yes
- Remarks:
- HPLC UV
- Buffers:
- pH 4
CHCOOH, 2 M 16.0 mL
CH3COONa 8 mL
Demineralised water ad 200 mL
pH is adjusted using NaOH or acetic acid, if necessary
pH 7
KH2PO4 1.7417 g
Demineralised water 100 mL
NaOH, 2 M 2.98 mL
Demineralised water ad 200 mL
pH is adjusted using NaOH or phosphoric acid, if necessary
pH 9
H3BO3 0.6186 g
KCl 0.7458 g
Demineralised water 100 mL
NaOH, 2 M 2.15 mL
Demineralised water ad 200 mL
pH is adjusted using NaOH or boric acid, if necessary - Duration:
- 120 h
- pH:
- 4
- Temp.:
- 50 °C
- Initial conc. measured:
- 250 mg/L
- Duration:
- 120 h
- pH:
- 7
- Temp.:
- 50 °C
- Initial conc. measured:
- 250 mg/L
- Duration:
- 120 h
- pH:
- 9
- Temp.:
- 50 °C
- Initial conc. measured:
- 250 mg/L
- Duration:
- 10 h
- pH:
- 10
- Temp.:
- 80 °C
- Remarks:
- pH 10-11; A 15% RR120 solution was prepared, and then one drop of that solution was diluted in 10 mL 50:50 MeOH:DI water
- Number of replicates:
- 2/pH
- Positive controls:
- yes
- Remarks:
- an additional sample was hydrolysed at pH 10-11 to identify the degradation products
- Statistical methods:
- NA
- Preliminary study:
- Results see below
- Transformation products:
- yes
- No.:
- #1
- No.:
- #2
- pH:
- 4
- Temp.:
- 50 °C
- Remarks on result:
- not measured/tested
- Remarks:
- see table for results
- pH:
- 7
- Temp.:
- 50 °C
- Remarks on result:
- not measured/tested
- Remarks:
- see table for results
- pH:
- 9
- Temp.:
- 50 °C
- Remarks on result:
- not measured/tested
- Remarks:
- see table for results
- pH:
- 10
- Temp.:
- 80 °C
- Remarks on result:
- not measured/tested
- Remarks:
- see table for results
- Conclusions:
- The core molecule (chromophore) is concluded to be hydrolytically stable
- Executive summary:
In a hydrolysis study performed acording to the protocol as described in OECD 111, the substance hydrolysed ca 35% at pH 4 and ca 10% pH 9. The hydrolysis products were identified. It was shown that the core molecule (chromophore) of the substance remains intact and the hydrolysis is related tothe chloride on the molecule.
The core molecule (chromophore) is therefore concluded to be hydrolytically stable
- Endpoint:
- hydrolysis
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 31 January 2018 to 05 February 2018
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 111 (Hydrolysis as a Function of pH)
- Principles of method if other than guideline:
- Only Tier 1 test
- GLP compliance:
- yes (incl. QA statement)
- Radiolabelling:
- no
- Analytical monitoring:
- yes
- Details on sampling:
- Solvent for stock solution: Demineralised water
Solvent for calibration: Demineralised water - Buffers:
- Buffer-Solution, pH 4
Parameter Amount
CH3COOH, 2 M 16.0 mL
CH3COONa 8 mL
Demineralised water ad 200 mL
pH is adjusted using NaOH or acetic acid, if necessary
Buffer-Solution, pH 7
Parameter Amount
KH2PO4 1.7417 g
Demineralised water 100 mL
NaOH, 2 M 2.98 mL
Demineralised water Ad 200 mL
pH is adjusted using NaOH or phosphoric acid, if necessary
Buffer-Solution, pH 9
Parameter Amount
H3BO3 0.6186 g
KCl 0.7458 g
Demineralised water 100 mL
NaOH, 2 M 2.15 mL
Demineralised water Ad 200 mL
pH is adjusted using NaOH or boric acid, if necessary - Details on test conditions:
- A test item stock solution with concentration 500 mg/L was prepared by dissolving 25 mg test item in 50 mL demineralised water. This solution was mixed 1:1 with the appropriate hydrolysis buffer solution, resulting a test concentration of 500 mg/L.
The test solutions were filtered over 0.2 µm PTFE filters directly into autoclaved HPLC vials, leaving only a small headspace. The vials were then purged with sterile Argon and quickly closed using PTFE coated seals. One vial of each pH was analysed directly, the remaining three vials were stored at 50 °C in an incubation chamber for five days. - Duration:
- 5 d
- pH:
- 4
- Temp.:
- 50 °C
- Initial conc. measured:
- >= 248.1 - <= 251 mg/L
- Remarks:
- analysed in duplicate
- Duration:
- 5 d
- pH:
- 7
- Temp.:
- 50 °C
- Initial conc. measured:
- >= 251.9 - <= 255 mg/L
- Remarks:
- analysed in duplicate
- Duration:
- 5 d
- pH:
- 9
- Temp.:
- 50 °C
- Initial conc. measured:
- >= 254.8 - <= 255.2 mg/L
- Remarks:
- analysed in duplicate
- Number of replicates:
- 4(1 anaysed on day 0 and 3 analysed on day 5)
- Positive controls:
- no
- Negative controls:
- yes
- Remarks:
- blanks were included
- Statistical methods:
- NA
- Preliminary study:
- Hydrolysis observed at pH 4 (-53.3%) and 9 (-9.5%)
- Transformation products:
- not measured
- pH:
- 4
- Temp.:
- 50 °C
- Remarks on result:
- not measured/tested
- pH:
- 7
- Temp.:
- 50 °C
- Remarks on result:
- not measured/tested
- pH:
- 9
- Temp.:
- 50 °C
- Remarks on result:
- not measured/tested
- Validity criteria fulfilled:
- yes
- Conclusions:
- No significant signs of hydrolysis were observed at pH 7. A decrease in test item concen-tration of 9.5 % was observed at pH 9 and a decrease of 53.3 % was observed at pH 4.
- Executive summary:
In a preliminary test at pH 4, 7 and 9 according to OECD 111, No significant signs of hydrolysis were observed at pH 7. A decrease in test item concentration of 9.5 % was observed at pH 9 and a decrease of 53.3 % was observed at pH 4.
Referenceopen allclose all
The results of the OECD Guideline 111 study
|
Reactive Red 120 (area % HPLC) |
Hydrolysis Product 1 (area % HPLC) |
Hydrolysis Product 2 (area % HPLC) |
pH 4 – a |
67.7 |
28.6 |
3.7 |
pH 4 – b |
65.5 |
30.4 |
4.1 |
pH 7 – a |
94.6 |
4.3 |
1.1 |
pH 7 – b |
95.8 |
3.1 |
1.1 |
pH 9 – a |
88.8 |
9.2 |
2.0 |
pH 9 – b |
88.6 |
9.8 |
1.6 |
Hydrolysis Study of Reactive Red 120 at 80°C at a pH range of 10-11
Time (Hours) |
Reactive Red 120 (area % HPLC) |
Hydrolysis Product 1 (area % HPLC) |
Hydrolysis Product 2 (area % HPLC) |
Initial |
100.0 |
|
|
2 |
84.9 |
15.1 |
|
4 |
40.5 |
29.8 |
29.7 |
8 |
7.2 |
15.5 |
77.3 |
10 |
1.6 |
1.2 |
97.2 |
Mean Concentrations [mg/L] |
|||
pH 4 |
pH 7 |
pH 9 |
|
t = 0 h |
249.6 |
253.5 |
255.1 |
t = 120 h |
116.6 |
253.1 |
231.0 |
Recovery after 120 h [%] |
46.7 |
99.9 |
90.5 |
Change over 120h[%] |
-53.3 |
-0.1 |
-9.5 |
Description of key information
In a preliminary test at pH 4, 7 and 9 according to OECD 111, No significant signs of hydrolysis were observed at pH 7. A decrease in test item concentration of 9.5 % was observed at pH 9 and a decrease of 53.3 % was observed at pH 4 (Laus 2018).
In a hydrolysis study performed acording to the protocol as described in OECD 111, the substance hydrolysed ca 35% at pH 4 and ca 10% pH 9. The hydrolysis products were identified. It was shown that the core molecule (chromophore) of the substance remains intact and the hydrolysis is related to the chloride on the molecule. The core molecule (chromophore) is therefore concluded to be hydrolytically stable (Kemira 2018)
Key value for chemical safety assessment
- Half-life for hydrolysis:
- 1 yr
- at the temperature of:
- 50 °C
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.