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EC number: 694-898-7 | CAS number: 23121-00-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 26 - 29 Apr 2016
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 016
- Report date:
- 2016
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- other: Councli Regulation (EC) No. 440/2008
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- Hess. Ministerium für Umwelt, Energie, Landwirtschaft und Verbraucherschutz, Wiesbaden, Germany (18 Nov 2014)
Test material
- Reference substance name:
- 4-(2-phenylpropan-2-yl)phenyl 4-oxo-3-(λ⁵-diazynylidene)-3,4-dihydronaphthalene-1-sulfonate
- Cas Number:
- 23121-00-8
- Molecular formula:
- C25H20N2O4S
- IUPAC Name:
- 4-(2-phenylpropan-2-yl)phenyl 4-oxo-3-(λ⁵-diazynylidene)-3,4-dihydronaphthalene-1-sulfonate
- Test material form:
- solid
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
- Remarks:
- The compound cannot be detected with standard analytical methods due to its low water solubility (0.073 mg/L, calculated with EPI Suite)
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: A stock preparation with a test item concentration of 100 mg/L was freshly prepared. For that purpose, a calibrated flask with test item and vehicle was treated in an ultrasonic device for 1 h. Subsequently, the preparation was stirred with a magnetic stirrer for further 23 h. Afterwards, the formulation was passed through a membrane filter with a pore size of 0.2 µm. The fitlrate was used for the study.
- Controls: Yes, consisting of reconstituted water without test substance.
- Evidence of undissolved material: The test medium was a clear preparation and stayed unchanged throughout the study.
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: water flea
- Source: IBACON GmbH, Roßdorf, Germany
- Age of parental stock: Parental daphnids are used for reproduction until they are about 6 weeks old. Thereafter, they are replaced by neonates. The clone is bred in the laboratories of the testing facility.
- Age of daphnids at test start: < 24 h old
- Culture conditions: Daphnia are kept individually in 100 mL glass vessels containing approximately 60 mL reconstituted water (ELENDT M4 medium) at a water temperature of 20 ± 2 °C and a 16 h light and 8 h dark regime to ensure similar conditions as in the experiment. Offspring are removed from the vessels at least twice per week.
- Feeding during test: No feeding during the test.
- Food type: Unicellular green algae Desmodesmus subspicatus
- Frequency: The parental daphnids are fed ad libitum three times per week.
METHOD FOR PREPARATION AND COLLECTION OF EARLY INSTARS OR OTHER LIFE STAGES:
Before start of the experimental phase, the young daphnids were separated from their parent animals and transferred into a test vessel containing reconstituted water. The daphnids were visually inspected for motility. If daphnids did not make any swimming movements within 15 s after their test vessels had been gently agitated, they were considered to be immobile and were not used for the study. At the start of the experimental phase the daphnids were cautiously removed using a pipette, separted from the reconstituted water using a fine-mesh sieve, and transferred into the test vessels containing test media (test item group) or reconstituted water (control group). The daphnids were randomly allocated to the different groups.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
Test conditions
- Test temperature:
- 19.3 - 20.1 °C
- pH:
- 7.75 - 7.82
- Dissolved oxygen:
- Control: 8.18 mg O2/L (0 h), 8.07 mg O2/L (48 h)
Treatment: 5.63 mg O2/L (0 h), 7.72 mg O2/L (48 h) - Nominal and measured concentrations:
- Control and 100 mg/L (nominal)
- Details on test conditions:
- TEST SYSTEM
- Test vessel: tubes
- Type: open
- Material, size, headspace, fill volume: 20 mL glass tubes filled with 20 mL (4 mL per daphnid)
- Aeration: No aeration during exposure.
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Fully demineralized water (Milli-Q Water Purification System, Merck Millipore, Darmstdt, Germany)
- Culture medium different from test medium: Culture medium same as test medium (reconstituted water: Elendt M4).
- Intervals of water quality measurement: pH and oxygen concentration of the test media were measured at the start and at the end of the exposure period. At the beginning of exposure, separate beakers, one for each test group but without dapnids, were used for the measurments. For the measurements at the end of the exposure period, the test media of each test group were pooled prior to measurement. The water temperature was measured in a separate vessel containing fully demineralized water only, kept under the same experimental conditions. The data were recorded using MCPS.
OTHER TEST CONDITIONS
- Adjustment of pH: No
- Photoperiod: 16 h light/8 h dark
- Light intensity: 599 lux (prior test) and 611 lux (at test end)
EFFECT PARAMETERS MEASURED:
Immobilization: Recorded after 24 and 48 h.
RANGE-FINDING STUDY
- Test concentrations: 100 mg/L
- Results used to determine the conditions for the definitive study: In a pre-test no immobilization was observed at a concentration of 100 mg/L under open static conditions. Therefore, the solution of a nominal test item concentration of 100 mg/L was tested in the present study. - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 0.073 mg/L
- Nominal / measured:
- estimated
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other:
- Remarks:
- Value refers to max. water solubility calculated with EPI Suite.
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat. (dissolved fraction)
- Basis for effect:
- mobility
- Details on results:
- - Behavioural abnormalities: No
- Mortality of control: 0%
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: The test medium was a clear preparation and stayed unchanged throughout the study. - Results with reference substance (positive control):
- - Results with reference substance valid? Yes, results of the reference substance (20 - 22 Oct 2015) were within the required range defined by the guideline.
- Relevant effect levels: EC50 (48 h) = 0.602 mg/L Potassium dichromate, 95% confidence interval: 0.525 - 0.689 mg/L
Any other information on results incl. tables
Under the conditions of the present study, an aqueous solution of nominal 100 mg/L of test substance revealed no aquatic toxicity in the test system.
The EC50 (48 h) was > 0.073 mg/L (nominal > 100 mg/L) and, thus, could not be determined in this study.
The value 0.073mg/L corresponds to the calculated water solubility (EPI Suite).
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.