4-hydroxy-3-methoxycinnamic acid

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

disregarded due to major methodological deficiencies

Study period:

2011

Reliability:

4 (not assignable)

Rationale for reliability incl. deficiencies:

other: publication with no enough detail to allow a conclusion on the reliability. Furthermore the EC50 is expressed as % and this percentage cannot be linked to a concentration in mg/L.

Qualifier:

no guideline followed

Principles of method if other than guideline:

Snails attractant pellets (SAP) were prepared from binary combination of carbohydrate + amino acid (20 mM) in 2% agar solution with active molluscicidal component Ferula asafoetida (ferulic acid, umbelliferone), Syzygium aromaticum (eugenol), Carum carvi (limonene).
The mortality data were observed after every 24h up to 96h.

GLP compliance:

no

Analytical monitoring:

no

Vehicle:

yes

Test organisms (species):

other aquatic mollusc: Lymnaea acuminata

Details on test organisms:

Adult L. acuminata (2.25±0.20 cm in length)

Test type:

other:

Water media type:

freshwater

Total exposure duration:

96 h

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

1.17 other: %

Nominal / measured:

nominal

Basis for effect:

mortality

Details on results:

The mortality data were observed after every 24h up to 96h. Lethal values (LC50), lower and upper confidence limits (LCL and UCL), slope values, t-ratio, “g” value and heterogeneity factor
were calculated.
The 96h LC50 of different SAP containing umbelliferone (96h LC50- 0.93%), limonene (96h LC50- 0.74%) was higher than of eugenol and ferulic acid 1.03%, 1.17%, respectively.

Validity criteria fulfilled:

not applicable

Conclusions:

The 96h LC50 of different snail attractant pellets (SAP) containing umbelliferone (96h LC50- 0.93%), limonene (96h LC50- 0.74%) was higher than of eugenol and ferulic acid 1.03%, 1.17%, respectively.

Executive summary:

In this study, the effect of several active molluscicidal component were investigated.

Snails attractant pellets (SAP) were prepared from binary combination of carbohydrate + amino acid (20 mM) in 2% agar solution with active molluscicidal component Ferula asafoetida (ferulic acid, umbelliferone), Syzygium aromaticum (eugenol), Carum carvi (limonene).

The mortality data were observed after every 24h up to 96h.

The 96h LC50 of different snail attractant pellets (SAP) containing umbelliferone (96h LC50- 0.93%), limonene (96h LC50- 0.74%) was higher than of eugenol and ferulic acid 1.03% and 1.17%, respectively.

Description of key information

Only one study close to the aim of the OECD standard guideline and useful for the REACh requirement hasard assessment was found.

In this study, the molluscicidal effect of the ferulic acid was investigated using Snails attractant pellets (SAP) which were prepared with different active molluscicidal components including ferulic acid. The mortality data were observed after every 24h up to 96h.

The 96h LC50 of different snail attractant pellets (SAP) containing umbelliferone (96h LC50- 0.93%), limonene (96h LC50- 0.74%) was higher than of eugenol and ferulic acid 1.03% and 1.17%, respectively.

In this study, it is not possible to determine the concentration in mg/L of the amount of ferulic acid used. The concentration is expressed as % and this percentage cannot be linked to a concentration in mg/L. Therefore no key value can be determined.