Lauric acid, ester with hydroxypropanediyl diacetate

Long-term toxicity to aquatic invertebrates

Description of key information

No effects up to the limit of water solubility (EU Method C.20), read across.

Additional information

Since no studies investigating the long-term toxicity of Lauric acid ester with hydroxypropanediyl diacetate (CAS 30899-62-8) to aquatic invertebrates are available, in accordance to Regulation (EC) No 1907/2006 Annex XI, 1.5 a read-across to the structurally related source substance Glycerides, C8-21 and C8-21-unsatd. mono- and di-, acetates (CAS 97593-30-1) was conducted.

The target substance is mainly characterized as a triester of glycerol esterified with acetic acid and lauric acid. A structurally very similar substance was selected as source substance. Glycerides, C8-21 and C8-21-unsatd. mono- and di-, acetates (CAS 97593-30-1) is a multi-constituent substance described as a triester of glycerol with acetic acid and lauric acid in a slightly different composition compared to the target substance. This read-across is justified in detail in the overall summary (IUCLID Section 6.1) and within the analogue justification in IUCLID Section 13. In this case of read-across, the best suited (highest degree of structural similarity, nearest physico-chemical properties) read-across substance was used for the assessment.

The chronic toxicity of the source substance Glycerides, C8-21 and C8-21-unsatd., mono- and di-, acetates (CAS 97593-30-1) to aquatic invertebrates was investigated in a study by Richter (2012). This study was conducted according to EU Method C.20 (GLP). Daphnia magna was exposed to the test substance for 21 days within a semi-static water regime at loading rates of 2.1, 4.5 and 10 mg/L. The concentrations of all loading rates were verified by GC and DOC analysis. GC analysis of dissolved and undissolved test solution at 10 mg/L before the final filtration step resulted in test concentrations close to nominal values. During the testing period (performed with the filtered test solutions, without the undissolved fraction), measured concentrations remained below the limit of detection (LOD) of both GC (0.070 mg/L) and DOC (2 mg/L) methods. The water solubility of the substance was tested at 100 and 1000 mg/L, resulting in values of 3.5 and 21.9 mg/L, respectively. Nevertheless, differences in solubility of substances in pure water (water solubility test) and test medium (aquatic test) can be expected.
After 21 days of exposure, no effects on survival or reproduction were observed, resulting in a NOELR (21 d) of ≥ 10 mg/L.

Based on the available result from one structurally related source substance (in accordance to Regulation (EC) No 1907/2006 Annex XI, 1.5) which is characterized by a comparable structure and composition, it can be concluded that Lauric acid ester with hydroxypropanediyl diacetate will not exhibit long-term effects to aquatic invertebrates up to the limit of water solubility.