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Diss Factsheets

Toxicological information

Eye irritation

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Administrative data

Endpoint:
eye irritation: in vivo
Type of information:
experimental study
Adequacy of study:
key study
Study period:
23 March to 14 April 1982
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
comparable to guideline study with acceptable restrictions

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1982
Report date:
1982

Materials and methods

Test guideline
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 405 (Acute Eye Irritation / Corrosion)
GLP compliance:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
Isopentyl phenethyl ether
EC Number:
259-943-3
EC Name:
Isopentyl phenethyl ether
Cas Number:
56011-02-0
Molecular formula:
C13H20O
IUPAC Name:
isopentyl phenethyl ether
Specific details on test material used for the study:
Test material name (as stated in the report): ANTHER
Appearance: Clear colourless liquid

Test animals / tissue source

Species:
rabbit
Strain:
New Zealand White
Details on test animals or tissues and environmental conditions:
New Zealand White rabbits weighing at least one Kilogram and aged at least 7 weeks, bought from a commercial supplier are used in the rabbit eye irritation test. Animals are kept before and during the test singly in cages from which all hay, straw, or similar potentially irritant material is excluded. They are given water, and a commercially available, pelleted diet ad libitium. The animals are in a good health at the start of testing. The animal number and its sex, age and weight at the start of testing are recorded.

Test system

Vehicle:
unchanged (no vehicle)
Controls:
yes
Amount / concentration applied:
0.1 ml
Duration of treatment / exposure:
instillation
Observation period (in vivo):
21 days after treatment
Details on study design:
Test substance is applied to one eye of each of 3 rabbits by gently pulling the lower lid away from the eye ball and placing 0.1 ml in the sac so formed. Eyes are examined 24 hours after treatment and thereafter at daily intervals and graded for corneal, conjunctival and iridial damage. Eyes are examined before application of materials and at daily intervals afterwards with a slit lamp and corneal swelling is measured.

Results and discussion

In vivo

Resultsopen allclose all
Irritation parameter:
cornea opacity score
Basis:
animal: 643
Time point:
24/48/72 h
Score:
0.83
Max. score:
1
Reversibility:
fully reversible within: 21 days
Irritation parameter:
cornea opacity score
Basis:
animal: 644
Time point:
24/48/72 h
Score:
0.83
Max. score:
1
Reversibility:
fully reversible within: 10 days
Irritation parameter:
cornea opacity score
Basis:
animal: 645
Time point:
24/48/72 h
Score:
0.66
Max. score:
1
Reversibility:
fully reversible within: 8 days
Irritation parameter:
iris score
Basis:
animal: 643, 644 and 645
Time point:
24/48/72 h
Score:
0
Max. score:
0
Reversibility:
other: score 0
Irritation parameter:
conjunctivae score
Remarks:
Redness
Basis:
animal: 643
Time point:
24/48/72 h
Score:
1.33
Max. score:
2
Reversibility:
fully reversible within: 21 days
Irritation parameter:
conjunctivae score
Remarks:
Redness
Basis:
animal: 645
Time point:
24/48/72 h
Score:
1.33
Max. score:
2
Reversibility:
fully reversible within: 5 days
Irritation parameter:
chemosis score
Basis:
animal: 643 and 644
Time point:
24/48/72 h
Score:
1
Max. score:
1
Reversibility:
fully reversible within: 8 days
Irritation parameter:
chemosis score
Basis:
animal: 645
Time point:
24/48/72 h
Score:
0.66
Max. score:
1
Reversibility:
fully reversible within: 8 days

Any other information on results incl. tables

Only superficial damage to the cornea was caused with complete healing in 5 days. Conjunctivis was slight and of 3 days duration.

Applicant's summary and conclusion

Interpretation of results:
GHS criteria not met
Conclusions:
The Anther was considered to be not Eye irritant according to this test. It can be concluded from the test results that the test substance ANTHER does not meet the criteria to be classified according to the CLP Regulation 1272/2008/EC.
Executive summary:

The Anther was examined for eye irritation potential in this Screen test, Rabbit eye test dated on 23 April 1982, performed at Unilever research Laboratory.

Test substance is applied to one eye of each of 3 rabbits by gently pulling the lower lid away from the eye ball and placing 0.1 ml in the sac so formed. Eyes are examined 24 hours after treatment and thereafter at daily intervals and graded for corneal, conjunctival and iridial damage. Eyes are examined before application of materials and at daily intervals afterwards with a slit lamp and corneal swelling is measured.

It can be concluded from the test results that the test substance ANTHER does not meet the criteria to be classified according to the CLP Regulation 1272/2008/EC.