Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 228-412-8 | CAS number: 6262-07-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Hydrolysis
Administrative data
Link to relevant study record(s)
- Endpoint:
- hydrolysis
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 09.08.2016 - 15.08.2016
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.7 (Degradation: Abiotic Degradation: Hydrolysis as a Function of pH)
- Version / remarks:
- Council regulation (EC) No 440/2008. Published in OJ No L 142/518 May 2008
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 111 (Hydrolysis as a Function of pH)
- Version / remarks:
- Adopted April 13, 2004
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Buffers:
- The hydrolysis of test substance was examined at buffered solutions of selected pH value: 4.0, 7.0 and 9.0. The actual pH value at given temperature was measured using a calibrated pH meter. All prepared buffer solutions were sterilized in an autoclave (121°C, 50 min) and purged by nitrogen. Buffer pH 4: 500mL 0.1 M potassium hydrogen phthalate + 4.0 mL 0.1 M NaOH / 1000 mL UPWpH 4.03 at 25.4°CBuffer pH 7: 296 mL 0.1M NaOH + 500 mL 0.1 M potassium dihydrogen phosphate/ 1000 mL UPWpH 7.02 at 25.4°C Buffer pH 9: 213 mL 0.1 M NaOH + 500 mL 0.1 M KCl in 0,1 M H3BO3 / 1000 mL UPW.pH 8.96 at 25.4°C
- Details on test conditions:
- TEST SYSTEM - Type, material and volume of test flasks, other equipment used: volumetric flask, the body of each flask was wrapped in an aluminium foil and tightly closed - Sterilisation method: all glassware, reagent-grade water and buffer solutions were sterilised by autoclave (121°C, 50 min) - Measures to exclude oxygen: oxygen is removed from a solution by purging with nitrogen TEST MEDIUM - Kind and purity of water: Ultrapure Water (UPW) – prepared in house by water purification system; Water purification system IWA 20iol, Watek CALIBRATION SOLUTIONApproximately 1.6 g of Acid Black 26 was weighed into a 100 mL volumetric flask, 1mL of methanol was added and then dissolved with UPW in an ultrasonic bath for 5 minutes and diluted to volume (stock solution - SS). This stock solution was diluted to four more concentrations (calibration solutions - CS) in this manner:CS No.1: 0.3 mL SS/100mL, nominal concentration of AB26 - 50 mg/LCS No.2: 0.4 mL SS/100mL, nominal concentration of AB26 - 64 mg/LCS No.3: 0.5 mL SS/100mL, nominal concentration of AB26 - 80 mg/LCS No.4: 0.6 mL SS/100mL, nominal concentration of AB26 - 100 mg/L SAMPLE SOLUTION0.4 mL of SS was pipetted into a 100 mL volumetric flask and replenished to the volume with a buffered solution of certain pH (4.0, 7.0, or 9.0) – in duplicate. A nominal concentration of 64 mg/L was prepared for preliminary test (Tier 1).During the experimental part of hydrolysis, the volumetric flasks were placed in the thermostatic bath kept at a given temperature. At certain period of time, a portion of sample solution was transferred into a vial followed by a quick cooling to room temperature.OTHER TEST CONDITIONS - Adjustment of pH: The actual pH value of each buffer was measured. The pH-meter calibrated to buffer solutions CertiPUR was used.
- Duration:
- 5.97 d
- pH:
- 4.06
- Temp.:
- 50 °C
- Duration:
- 5.97 d
- pH:
- 6.98
- Temp.:
- 50 °C
- Duration:
- 5.97 d
- pH:
- 8.84
- Temp.:
- 50 °C
- Number of replicates:
- 2 replicates
- Positive controls:
- no
- Negative controls:
- no
- Preliminary study:
- The preliminary test has shown that the test substance was not hydrolyzed by more than 10% in period of 5 days at pH 4, 7 and 9.
- Transformation products:
- no
- % Recovery:
- > -7.2 - < -6.5
- pH:
- 4.06
- Temp.:
- 50 °C
- Duration:
- 5.97 d
- % Recovery:
- > -4.4 - < -0.7
- pH:
- 6.98
- Temp.:
- 50 °C
- Duration:
- 5.97 d
- % Recovery:
- > -9.8 - < -1.4
- pH:
- 8.84
- Temp.:
- 50 °C
- Duration:
- 5.97 d
- Key result
- pH:
- 4.06
- Temp.:
- 25 °C
- DT50:
- > 1 yr
- Remarks on result:
- hydrolytically stable based on preliminary test
- Key result
- pH:
- 6.98
- Temp.:
- 25 °C
- DT50:
- > 1 yr
- Remarks on result:
- hydrolytically stable based on preliminary test
- Key result
- pH:
- 8.84
- Temp.:
- 25 °C
- DT50:
- > 1 yr
- Remarks on result:
- hydrolytically stable based on preliminary test
- Details on results:
- TEST CONDITIONS - pH, sterility, temperature, and other experimental conditions maintained throughout the study: Yes
- Validity criteria fulfilled:
- yes
- Conclusions:
- The preliminary test has shown that the test substance was not hydrolyzed by more than 10% in period of 5 days at pH 4, 7 and 9. Therefore the test substance was considered to be hydrolytically stable at above pHs. It can be assumed that the half-time at 25 °C would be > 1 year.
- Executive summary:
The procedure was performed according to: Method C.7 – Degradation – Abiotic Degradation: Hydrolysis as a Function of pH, Council regulation (EC) No 440/2008. Published in OJ No L 142/518 May 2008 and OECD Test Guidline No.111, Hydrolysis as a function of pH, Adopted April 13, 2004.
The preliminary test has shown that the test substance was not hydrolyzed by more than 10% in period of 5 days at pH 4, 7 and 9 (see Table 1), therefore the test substance was considered to be hydrolytically stable at above pHs. It can be assumed that the half-time at 25 °C would be > 1 year.
Reference
Table 1: Summary of results - Tier 1
Date | Day | pH 4.06 at 50°C | pH 6.98 at 50°C | pH 8.84 at 50°C | |||
1 | 2 | 1 | 2 | 1 | 2 | ||
9.8.2016 | 0 – start | 0.0% | 0.0% | 0.0% | 0.0% | 0.0% | 0.0% |
10.8.2016 | 0.8 | -8.2% | -7.1% | -1.5% | -3.5% | -8.5% | 1.1% |
11.8.2016 | 1.92 | -6.0% | -6.0% | 1.5% | -2.6% | -7.9% | 2.4% |
12.8.2016 | 2.78 | -3.3% | -5.9% | 0.4% | -3.7% | -9.2% | 3.1% |
14.8.2016 | 4.96 | -7.0% | -5.2% | 1.0% | -2.8% | -8.2% | 1.2% |
15.8.2016 | 5.97 | -6.5% | -7.2% | -0.7% | -4.4% | -9.8% | -1.4% |
Description of key information
The preliminary test has shown that the test substance was not hydrolyzed by more than 10% in period of 5 days at pH 4, 7 and 9. Therefore the test substance was considered to be hydrolytically stable at above pHs. It can be assumed that the half-time at 25 °C would be > 1 year.
Key value for chemical safety assessment
- Half-life for hydrolysis:
- 1 yr
- at the temperature of:
- 25 °C
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.