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EC number: 215-236-1 | CAS number: 1314-56-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- migrated information: read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Study period:
- 2008
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: OECD Guideline conform GLP study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 008
- Report date:
- 2008
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Deviations:
- no
- GLP compliance:
- yes
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Orthophosphoric acid
- EC Number:
- 231-633-2
- EC Name:
- Orthophosphoric acid
- Cas Number:
- 7664-38-2
- IUPAC Name:
- phosphoric acid
- Details on test material:
- Purity: > 99.999%;
Supplier: Sigma-Aldrich, Inc.;
Lot No.: 09823ME
Constituent 1
Method
Species / strainopen allclose all
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Species / strain / cell type:
- E. coli WP2 uvr A
- Metabolic activation:
- with and without
- Metabolic activation system:
- rat liver S9 mix
- Test concentrations with justification for top dose:
- 156.3, 312.5, 625, 1,250 and 5,000 μg/plate
- Vehicle / solvent:
- Sterilized distilled water;
Lot No.: 400644;
Manufacturer: GIBCO 15230-147
Controls
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- Remarks:
- Sterilized distilled water
- Positive controls:
- yes
- Positive control substance:
- other: sodium azide 2-Nitrofluorene, 9-Aminoacridine, 2-Aminoanthracene, 2-Aminofluorene
- Details on test system and experimental conditions:
- Six concentrations of the test substance were tested in triplicate against each tester strain, using a plate incorporation method.1) Master plate was prepared from the frozen permanent.2) A colony was selected from the master plate and inoculated to 10 ml of 1.6 % Oxoid Nutrient Broth (pH 7.0~7.5) for 12~14 hr by shaking at 200 rpm.3) The test substance (or vehicle, or positive controls) 0.1 ml , 0.1 ml og bacterial cultres, and 0.5 ml of S9 mix (or sodium phosphate buffer, 0.2 M, pH 7.4) were added to a test tube and then, 2 ml of molten top agar containing trace histidine or tryptophan was added to each tube. The mixture was overlaid onto sterile plates of Vogel-Bonner Minimal glucose agar.4) After approximately 48 hr incubation at 37 °C the frequency of revertant colonies was assessed.5) In order to assess the sterility of the test substance and S9 mix, 0.1 ml of the test substance at a maximal concentration (or 0.5 ml of S9 mix) and 2 ml of molten top agar were overlaid onto a sterile minimal glucose agar plate.6) Three plates was used at each dose levels.7) The plate was selected randomly in order to avoid a confounding effects.8) The project number, the name of bacteria strain, the treatment dose and absence or presence of S9 mix were recorded on the bottom of plate.
- Evaluation criteria:
- Data were presented using tables and figures as individual plate counts, the mean number of revertant colonies per plate and standard deviation.The following several criteria were used to determine a positive result.1) The case that under the S9- or S9+ condition, the increase of reverse mutation colony of a strain in a dose-dependent manner, detection of repeated increase at doses more than one concentration, or having a significant linear relation.Biological relevance of the results was considerd.2) An increase in revertant colonies over two times in bacterial strains TA98, TA100 and WP2uvrA, and over three times in strains TA1535 and TA1537 than those in negative control group.Besides the above cases, it was judged negative. Statistical significancy-analysis was not carried out.
- Statistics:
- none
Results and discussion
Test resultsopen allclose all
- Species / strain:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- at 5,000 μg/plate in TA100 strain with S9 mix
- Species / strain:
- E. coli WP2 uvr A
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- at 5,000 μg/plate in TA100 strain with S9 mix
- Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Any other information on results incl. tables
Table1: Summary of revertant colony numbers obtained per plate with/without S9 mix
S9 mix (5 %) |
Dose (ug/plate)* |
Number of revertant colonies/plate |
||||
Base replacement type |
Frame shift type |
|||||
TA100 |
TA1535 |
WP2uvrA |
TA98 |
TA1537 |
||
- |
NC |
165+/-6 |
15+/-3 |
24+/-1 |
17+/-2 |
9+/-2 |
156.3 |
173+/-2 |
20+/-2 |
25+/-5 |
19+/-4 |
6+/-2 |
|
312.5 |
163+/-10 |
15+/-2 |
19+/-6 |
21+/-5 |
5+/-3 |
|
625 |
153+/-6 |
17+/-6 |
20+/-5 |
16+/-3 |
7+/-1 |
|
1250 |
158+/-6 |
17+/-6 |
14+/-2 |
16+/-3 |
5+/-1 |
|
2500 |
165+/-10 |
15+/-1 |
20+/-6 |
17+/-3 |
9+/-3 |
|
5000 |
166+/-2 |
11+/-4 |
23+/-7 |
20+/-7 |
5+/-4 |
|
PC |
479+/-15 |
520+/-82 |
122+/-8 |
174+/-29 |
771+/-21 |
|
+ |
NC |
174+/-5 |
11+/-2 |
22+/-3 |
29+/-6 |
11+/-0 |
156.3 |
161+/-16 |
13+/-4 |
18+/-3 |
25+/-10 |
6+/-3 |
|
312.5 |
156+/-5 |
15+/-5 |
19+/-4 |
25+/-8 |
8+/-2 |
|
625 |
160+/-7 |
18+/-5 |
16+/-3 |
25+/-8 |
10+/-3 |
|
1250 |
159+/-8 |
16+/-5 |
28+/-8 |
30+/-3 |
9+/-1 |
|
2500 |
154+/-26 |
18+/-2 |
18+/-7 |
18+/-4 |
9+/-2 |
|
5000 |
1+/-1 |
1+/-1 |
23+/-4 |
0+/-0 |
1+/-1 |
|
PC |
587+/-30 |
144+/-22 |
329+/-37 |
493+/-59 |
317+/-31 |
Data are presented as mean+/-SD (N=3)
NC: negative control (Sterilized distilled water, 100 ul/plate*)
PC: positive control
*ug/plate, ul/plate: micro g/plate, micro l/plateApplicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative
Diphosphorus pentaoxide is a hygroscopic solid which forms with moisture/water an aqueous solution of phosphorus oxyacids (primary hydrolysis) that are subject to further (secondary) hydrolysis to the end product phosphoric acid, H3PO4. Therefore, the genotoxicity of Diphosphorus pentaoxide is solely based on secondary effects of Phosphoric acid and an read across to this substance is justified.
Under the present test conditions Phosphoric acid was considered to be non-genotoxic. - Executive summary:
Introduction
This study was conducted to evaluate the mutagenic potential of the test substance, phosphoric acid using a bacterial system. This study was performed in accordance with the procedures described in the following internationally accepted guidelines and recommendations: OECD Guidelines for Testing of Chemicals (Jul. 21, 1997) No. 471[ Bacterial Reverse Mutation Test] and [Genotoxicity Test] described in the TCCA-Good Laboratory Practice Standards and Test Guideline (NIER Public Notice No. 2007-29) issued by Korean National Institute of Environmental Research.
Dose range-finding test
Dose range-finding test was performed using theSalmonella typhimuriumstrains TA98, TA100 and theEscherichia colistrain WP2uvrA in the presence and absence of a metabolic activation containing S9 fraction (rat liver postmitochondrial fraction). The test substance at 5000 ug/plate* was used as a maximal concentrations in the absence and presence of S9 mix, in the main mutation test, and diluted to six doses (156.3, 312.5, 625, 1,250 and 5,000 ug/plate) by a factor two.
Bacterial reverse mutation test
In the main and the confirmation test, five bacterial strains,Salmonella typhimuriumTA98, TA100, TA1535, TA1537 andEscherichia coliWP2uvrA were treated with the test substance with or without S9 mix using a plate incorporation method and 3 plates at each dose levels were used (triplicates). As a result of the main mutation test, the test substance at any concentrations of 156.3~5,000 ug/plate with or without S9 mix didnt induce the increase in the frequency of revertant colonies in a dose-dependent manner, which representing non-genotoxic.
Regardless of S9 mix and tester strains, the precipitation of the test substance was not observed on the agar plate treated at any doses. In the confirmation test, the test substance at same concentrations of the main mutation test with or without S9 mix didnt induce the increase in the frequency of revertant colonies dose-related. The precipitation of the test substance was not observed on the agar plate treated at any doses.
The frequency of revertant colonies for the negative control, sterilized distilled water was considered to be acceptable and all of the positive controls induced marked increases in the frequency of revertant colonies confirming the activity of S9 mix and the sensitivity of the bacterial strains.
Conclusion
The test substance, phosphoric acid was considered to be non-genotoxic under the present test conditions.
*ug/plate: micro g/plate
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