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EC number: 203-400-5 | CAS number: 106-46-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Guideline study.
Data source
Reference
- Reference Type:
- publication
- Title:
- Unnamed
- Year:
- 1 993
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- GLP compliance:
- yes
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- 1,4-dichlorobenzene
- EC Number:
- 203-400-5
- EC Name:
- 1,4-dichlorobenzene
- Cas Number:
- 106-46-7
- Molecular formula:
- C6H4Cl2
- IUPAC Name:
- 1,4-dichlorobenzene
- Test material form:
- other: solid
- Details on test material:
- IUCLID4 Test substance: other TS: purity > 99 %; solvent: DMSO
Constituent 1
Method
- Target gene:
- Ames assay: detection of base pair substitutions and frameshift mutations
Species / strain
- Species / strain / cell type:
- S. typhimurium, other: TA98, TA100, TA1535, TA1537, TA1538
- Additional strain / cell type characteristics:
- other: S. typhimuriumTA98, TA100, TA1535, TA1537, TA1538
- Metabolic activation:
- with and without
- Metabolic activation system:
- liver S9-mix from male Sprague-Dawley rats pretreated with i.p. injection of Aroclor in olive oil
- Test concentrations with justification for top dose:
- 37, 111, 333, 1000, 3000 µg/plate in DMSO
- Vehicle / solvent:
- DMSO
Controls
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- not specified
- Positive controls:
- yes
- Positive control substance:
- 2-nitrofluorene
- sodium azide
- other: 2-aminoanthracen, adriamycin
- Details on test system and experimental conditions:
- IUCLID4 Type: Ames test, Ames plate incorporation assay with and without metabolic activation, with 5 strains, investigations carried out in triplicate, positive and negative (solvent) control
- Evaluation criteria:
- positive
if at least one strain shows for 2 concentrationsa significant increase (p<5 %, reproducible ) over the respective negative (solvent) control or
if a positive dose effect relationship including 3 concentrations of factor 3 is shown - Statistics:
- student t-test
Results and discussion
Test results
- Species / strain:
- S. typhimurium, other: Salmonella typhimurium TA 98, TA 100, TA 1535, TA 1537, TA 1538
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not specified
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Remarks on result:
- other: other: Salmonella typhimurium TA 98, TA 100, TA 1535, TA 1537, TA 1538
- Remarks:
- Migrated from field 'Test system'.
Any other information on results incl. tables
Bacteria strain | metabol. | positive control | concentration |
TA 98 | without/with | Adiamycin/Aflatoxin B1 | 3 µg/plate / 1 µg/plate |
TA 100 | without/with | Adiamycin/Aflatoxin B1 | 3 µg/plate / 1 µg/plate |
TA 1535 | without/with | Sodium azide/2 -Aminoanthracene | 3 µg/plate / 1µg/plate |
TA 1537 | without/with | 2 -Nitrofluorene /2 -Aminoanthracene | 3 µg/plate / 1 µg/plate |
TA 1538 | without/with | 2 -Nitrofluorene/Aflatoxin B1 | 3 µg/plate / 1 µg/plate |
Negative controls reacted negative.
Positive controls reacted positive.
Applicant's summary and conclusion
- Executive summary:
1,4 -dichlorobenzene was investigated in accordance with OECD guidelines and under GLP conditions with regard to a possible mutagenic action on Salmonella typhimurium. The investigations were carried out as an Ames Plate Incorporation Assay with the strains Salmonella thyphimurium TA 98, TA 100, TA 1535, TA 1537 and TA 1538 in two independent tests per strain. The experiments comprised testing of the substance at five concentrations both without and with metabolic activation as well as positive and negative controls. All investigations were carried out in triplicate.
1,4 -dichlorobenzene did not prove to be mutagenic in the Ames test for the strains of Salmonella typhimurium tested.
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