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Diss Factsheets
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EC number: 203-400-5 | CAS number: 106-46-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Basic toxicokinetics
Administrative data
- Endpoint:
- basic toxicokinetics
- Type of information:
- experimental study
- Adequacy of study:
- other information
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: OECD guideline and GLP not defined
Data source
Reference
- Reference Type:
- publication
- Title:
- Unnamed
- Year:
- 1 991
Materials and methods
- Objective of study:
- metabolism
- Principles of method if other than guideline:
- 1. Precision-cut liver slices in dynamic organ culture, is described and applied to the study of hepatic drug metabolism in the rat.
2. Precision cut Human liver slices in dynamic organ culture have been used to study the integrated metabolism . - GLP compliance:
- not specified
Test material
- Reference substance name:
- 1,4-dichlorobenzene
- EC Number:
- 203-400-5
- EC Name:
- 1,4-dichlorobenzene
- Cas Number:
- 106-46-7
- Molecular formula:
- C6H4Cl2
- IUPAC Name:
- 1,4-dichlorobenzene
- Details on test material:
- >97% radiochemical purity
Constituent 1
- Radiolabelling:
- not specified
Test animals
- Species:
- other: rat/human
- Strain:
- other: Fischer-344
- Sex:
- male/female
Administration / exposure
- Route of administration:
- other:
- Vehicle:
- not specified
- Duration and frequency of treatment / exposure:
- see: Any other information on materials and methods
Doses / concentrations
- Remarks:
- Doses / Concentrations:
-
- No. of animals per sex per dose / concentration:
- -
- Control animals:
- not specified
Results and discussion
- Preliminary studies:
- see: executive summary
Toxicokinetic / pharmacokinetic studies
- Details on absorption:
- see: executive summary
- Details on distribution in tissues:
- see: executive summary
- Details on excretion:
- see: executive summary
Metabolite characterisation studies
- Details on metabolites:
- see: executive summary
Any other information on results incl. tables
see: executive summary
Applicant's summary and conclusion
- Executive summary:
1) Incubation of 0.5nM MCB withe male rat liver slices in dynamic organ culture resulted in the formation of aqueous soluble metabolites, but biotransformation activity was low. To increase the amount of metabolites formed, the number of slices per incubation was increased to three, and the volume of incubation medium was decreased from 7 to 3.5 ml. This modification resulted in only a two-fold increasein metabolism of MCB. All subsequent experiments with 0.5 mM MCB or DCB used three liver slices in 3.5 ml of incubation media. There was a time-dependent increase in the metabolism of MCB and DCB to aqueous soluble metabolites that were excreted into the incubation medium. Those metabolites retained by the slices were low and remained constant with time. Overall, the extent of metabolism of these compounds was low. At most, 5% of the added substrate was metabolized. Assessment of the sex differences in the metabolism of MCB revealed no significant differences in the extent of metabolism.Differential rates of metabolism were found between the isomers, with m->0 ->p-DCB=MCB. Differences in metabolism of the isomers could not be explained by differences in the partitioning of the parent compound into the slices. 30% of p-DCB partitioned into the slices. The percentage of total radiolabel found in the tissue for each compound was found to be constant over each 2h interval.
2) The metabolism of p-DCB to aqueous soluble metabolites:
The incubation of p-DCB with human liver slices in dynamic organ culture was carried out using the modified incubation technique employed in previous rat studies. P-DCB was incubated at a concentration of 0,5 mM with slices from three individual human livers. There was a time-dependent increase in the aqueous soluble metabolite concentration present in the medium. The levels of metabolites present in the liver slices remained constant over the incubation period.
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