Registration Dossier
Registration Dossier
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EC number: 203-401-0 | CAS number: 106-47-8
Biodegradation in water and sediment: simulation tests
Administrative data
- Endpoint:
- biodegradation in water and sediment: simulation tests
- Type of information:
- experimental study
- Adequacy of study:
- other information
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Acceptable, well documented publications which meets basic scientific principles
Data source
Reference
- Reference Type:
- publication
- Title:
- Effect of chemical structure on microbial degradation of substituted benzenes
- Author:
- Alexander M and Lustigman BK
- Year:
- 1 966
- Bibliographic source:
- J. Agr. Food Chem. 14, 410-413
Materials and methods
- Principles of method if other than guideline:
- photometrical measurement of ring cleavage; method according to
Alexander M & Aleem MIH (1961). J. Agr. Food Chem. 9, 44. - GLP compliance:
- not specified
Test material
- Reference substance name:
- 4-chloroaniline
- EC Number:
- 203-401-0
- EC Name:
- 4-chloroaniline
- Cas Number:
- 106-47-8
- Molecular formula:
- C6H6ClN
- IUPAC Name:
- 4-chloroaniline
- Details on test material:
- - Name of test material (as cited in study report): 4-chloroaniline
- Analytical purity: no data
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- other: soil microflora of Niagara silt loam
- Details on inoculum:
- 1 ml of a 1% suspension of Niagara silt loam
Initial test substance concentration
- Initial conc.:
- 25 mg/L
- Based on:
- test mat.
- Details on study design:
- - Nutrient solution contained inorganic nutrients and test substance as the
sole carbon source.
- 1 ml of a 1 % suspension of Niagara silt loam was added to closed bottle
containing 40 ml of nutrient solution.
- Bottles were incubated in the dark at 25 °C.
- Contact time was up to 64 days including adaptation period.
- Ring cleavage was checked by decrease of absorbance at 265 nm,
measured after centrifugation in the supernantant. Precipitates and
supernatants were returned to the appropriate reaction bottles.
- Control tests were performed with identical samples except that 8 mg of
HgCl2 and 5E-07 M Tween 80 were added to each bottle.
- Tests for toxicity of the test substances to microorganisms were done on
identical samples however using glucose as an additional source of
carbon.
Results and discussion
% Degradation
- % Degr.:
- ca. 0
- Parameter:
- other: ring cleavage
- Sampling time:
- 64 d
- Transformation products:
- not specified
Applicant's summary and conclusion
- Conclusions:
- In an with microbial cultures from soil, removal of PCA was negligible when non-acclimated inocula were used.
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