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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
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EC number: - | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 16/04/2009 to 14/05/2009
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Study conducted to GLP in accordance with recognised guideline
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 009
- Report date:
- 2009
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 C (Ready Biodegradability: Modified MITI Test (I))
- Deviations:
- no
- Principles of method if other than guideline:
- Not applicable.
- GLP compliance:
- yes (incl. QA statement)
Test material
- Details on test material:
- No further information required.
Constituent 1
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
Not applicable.
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, adapted
- Details on inoculum:
- - Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure):
- Laboratory culture: Activated sludge was collected, prepared and controlled by Chemicals Evaluation and Research Institute
- Method of cultivation: The activated sludge, which was cultivated for 18.5 hours after it had been added the synthetic sewage (glucose, peptone and potassium dihydrogenphosphate were dissolved in purified water, and the pH of the solution was adjusted to 7.0±1.0), was used.
- Storage conditions: At a temperature of 25±1°C under the conditions of darkness.
- Storage length: 28 days
- Preparation of inoculum for exposure: 3 mL of solutions A, B, C and D were made up to 1000 mL with purified water (Japanese Pharmacopeia, Takasugi Pharmaceutical Co., Ltd.), and then the pH of this solution was adjusted to 7.0.
- Concentration of sludge: Concentration of suspended solid in the activated sludge was 3280 mg/L.
- Water filtered: No - Duration of test (contact time):
- 28 d
Initial test substance concentration
- Initial conc.:
- 100 mg/L
- Based on:
- test mat.
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- TEST CONDITIONS
- Composition of medium: Not specified
- Additional substrate: Each of the test solutions were made up to 1000 mL with purified water.
- Solubilising agent (type and concentration if used): n/a
- Test temperature: 25±1°C
- pH: 7.0
- pH adjusted: Yes
- Aeration of dilution water: Not specified
- Suspended solids concentration: The activated sludge was added to each test vessel so that the concentration of the suspended solid reached 30 mg/L.
- Continuous darkness: Yes
TEST SYSTEM
- Culturing apparatus: Closed system oxygen consumption measuring apparatus
- Number of culture flasks/concentration: 3
- Method used to create aerobic conditions: Not specified
- Method used to create anaerobic conditions: n/a
- Measuring equipment: Closed system oxygen consumption measuring apparatus:
- Temperature controlled bath and measuring unit : Asahi Techneion Co., Ltd.
- Data sampler : Asahi Techneion Co., Ltd.
- Vessel: 300 mL in volume (Improved type)
- Absorbent for carbon dioxide: Soda lime No.1 (for absorption of carbon dioxide, Wako Pure Chemical Industries, Ltd.)
- Test performed in closed vessels due to significant volatility of test substance: No
- Test performed in open system: Not specified
SAMPLING
- Sampling frequency: Not specified
- Sampling method: Not specified
- Sample storage before analysis: Not specified
CONTROL AND BLANK SYSTEM
- Inoculum blank: In one test vessel, nothing was added to the basal culture medium
STATISTICAL METHODS:
-The concentration of DOC (Dissolved Organic Carbon) in the sample for TOC was calculated by subtracting concentration of the inorganic carbon (IC) from concentration of the total carbon (TC). The concentration of TC and IC in the test solutions was proportionally calculated from the peak area of the test solution by comparison with that of 80.0 mgC/L standard solution for TC analysis and 80.0 mgC/L standard solution for IC analysis, respectively.
-The concentration of the test item in the sample for HPLC analysis was calculated proportionally by comparing the peak area on the chromatogram of the sample for HPLC analysis with that on the chromatogram of 101 mg/L standard solution.
-Percentage biodegradation by BOD:
Percentage biodegradation (%) = (BOD - B/TOD) x 100
BOD = Biochemical oxygen demand in the test solution
B = Biochemical oxygen demand in the control blank
TOD = Theoretical oxygen demand required when the test item was completely oxidized
Percentage biodegradation by DOC:
Percentage biodegradation (%) = (DOCw - DOCs/DOCw) x 100
DOCs = Residual amount of the dissolved organic carbon in the test solution
DOCw = Residual amount of the dissolved organic carbon in the test solution
Percentage biodegradation of test item:
Percentage biodegradation (%) = (Sw - Ss/Sw) x 100
Ss = Residual amount of the test item in the test solution
Sw = Residual amount of the test item in the test solution
Reference substance
- Reference substance:
- aniline
Results and discussion
- Preliminary study:
- Not performed
- Test performance:
- The concentration of DOC corresponding to the minimum determination limit was regarded as 1.0 mg C/L.
The lowest detectable peak area of the test item was regarded as 50000 µV • sec considering the noise level, which corresponded to the test item concentration of 0.42 mg/L.
% Degradation
- Parameter:
- % degradation (O2 consumption)
- Value:
- 0 - 4
- Sampling time:
- 28 d
BOD5 / COD results
BOD5 / COD
- Parameter:
- BOD5
- Value:
- 0 - 2.2 mg O2/g test mat.
- Results with reference substance:
- Biodegradation of Analine at 28 days was 77%
Any other information on results incl. tables
The test item is a mixture which consists of many components 15310 ingredients, as such it was detected as two peaks that could divide on the HPLC chromatogram in the determination of the test item. The two peaks were referred to as peak 1 and 2 in elution order. The percentage biodegradation of each peak of the test item was in the range from 2 to 3%. From these results, it was considered that all of the components of the test item were not biodegraded by microorganisms under the test conditions of this study.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- under test conditions no biodegradation observed
- Conclusions:
- The test item was not biodegraded by microorganisms under the test conditions of this study.
- Executive summary:
In a biodegradation study of Ink BH11 C by microorganisms (CERI study number: 15304) the test material was not considered to have biodegraded under the conditions of the study.
The method followed that described in the OECD Guidelines for Testing of Chemicals, Guideline 301 C, Revised July 17, 1992,
Ready Biodegradability: Modified MITI Test (I).
The test item is a mixture which consists of many components 15310 ingredients, as such it was detected as two peaks that could divide on the HPLC chromatogram in thedetermination of the test item. The two peaks were referred to as peak 1 and 2 in elution order. The percentage biodegradation of each peak of the test item was in the range from 2 to 3%. From these results, it was considered that all of the components of the test item were not biodegraded by microorganisms under the test conditions of this study.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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