Propane, 1,1,1,2,3-pentachloro-

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Study period:

9. 9. - 27. 9. 2013

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Meets National standards method (ČSN EN ISO).

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2013

Report date:

2013

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

ISO 8692 (Water Quality - Fresh Water Algal Growth Inhibition Test with Scenedesmus subspicatus and Selenastrum capricornutum)

Deviations:

yes

Remarks:

In preliminary test was not fulfilled test quality criterion No.1. This deviation has not impact on the test, because result was calculated from limit test.

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

1,1,1,2,3-pentachloropropane

EC Number:

688-501-6

Cas Number:

21700-31-2

Molecular formula:

C3H3Cl5

IUPAC Name:

1,1,1,2,3-pentachloropropane

Details on test material:

- Name of test material (as cited in study report): 1,1,1,2,3-pentachloropropane
- Substance type: organic
- Physical state: liquid
- Analytical purity: 99.940 % (w/w)
- Impurities (identity and concentrations): chlorinated hydrocarbons - 0.048 % (w/w)
- Lot/batch No.: 2-750
- Expiration date of the lot/batch: august 2014
- Storage condition of test material: dry place at room temperature

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Details on test solutions:

The stock solution of the test substance was prepared in the test medium. 100 mg of the test substance was weighed into 1000 mL of the test medium for the preliminary and limit test. The concentrations of solutions used in the preliminary test were obtained by dilution of the stock solution with test medium.

Test organisms

Test organisms (species):

Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)

Details on test organisms:

TEST ORGANISM
- Common name: Desmodesmus subspicatus
- Strain: Brinkmann 1953/SAG 86.81
- Source (laboratory, culture collection): Botanic Institute of the Czech academy of Science, Třeboň

ACCLIMATION
- Acclimation period: 3 days
- Culturing media and conditions (same as test or not): same as at test

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

72 h

Test conditions

Test temperature:

21 - 24°C

pH:

7.5 - 7.7

Nominal and measured concentrations:

Nominal concentrations:
- preliminary test: 1, 5, 10, 50 and 100 mg/L
- limit test: 100 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: Erlenmeyer flask
- Material, size, headspace, fill volume: 50 mL of the test solution
- Aeration: no
- Initial cells density: 10000 cells/mL
- Control end cells density: Burker's counting chamber
- No. of vessels per concentration (replicates): 1 (preliminary test), 6 (limit test)
- No. of vessels per control (replicates): 1 (preliminary test), 6 (limit test)

GROWTH MEDIUM
- Standard medium used: yes

TEST MEDIUM / WATER PARAMETERS
- Conductivity: < 5 µS/cm

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: continuous white light
- Light intensity and quality: 4400 - 8800 lux

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: Burker's counting chamber
- Observation intervals: 0, 24, 48 and 72 hours

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Results and discussion

Effect concentrations

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Details on results:

see table No. 1 and 2

Results with reference substance (positive control):

The sensitivity of the test species and correctness of test performance were verified by testing the reference substance, potassium dichromate. The results of the verification test with potassium dichromate are the following:
ErC50 (72 h) = 0.95 mg/L (95% confidence limit: 0.75 – 1.15 mg/L)

From the results of the last interlaboratory test were obtained data for potassium dichromate ErC50 (72 h) generated at 14 laboratories. The following reference range of values could be calculated from those data:
Interlaboratory test data: ErC50 (72 h) = 0.50 – 1.18 mg/L
The value EC50 (72 h) for inhibition of growth rate obtained from thies reference test meets the calculated range from the interlaboratory test.

Any other information on results incl. tables

Table No. 1: Preliminary test

Nominal concentration (mg/L)	Cell density in 1 mL				Growth rate
	0 hours	24 hours	48 hours	72 hours	Mean growth	Reduction (%)
100	10000	12500	62500	331250	1.17	1.0
50	10000	12500	56250	337500	1.17	0.5
10	10000	25000	62500	343750	1.18	0.0
5	10000	25000	62500	337500	1.17	0.5
1	10000	31250	68750	387500	1.22	-3.4
Control	10000	25000	93750	343750	1.18	0.0

Table No. 2: Limit test

Nominal concentration (mg/L)	Cell density in 1 mL				Growth rate 0-72 h
	0 hours	24 hours	48 hours	72 hours		mean
100	10000	50000	118750	656250	1.39	1.39 (reduction 2.8 %)
	10000	62500	125000	625000	1.38
	10000	62500	118750	618750	1.38
	10000	62500	137500	556250	1.34
	10000	68750	125000	706250	1.42
	10000	50000	125000	681250	1.41
Control	10000	43750	106250	681250	1.41	1.43
	10000	43750	112500	718750	1.42
	10000	50000	100000	675000	1.40
	10000	50000	106250	762500	1.44
	10000	50000	106250	806250	1.46
	10000	43750	187500	687500	1.41

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

Alga, Growth Inhibition test was performed according to the standard ČSN EN ISO 8692 (2012), Kvalita vod – Zkouška inhibice růstu sladkovodních zelených řas. The mentioned testing procedure is accredited as test method T20 in the testing laboratory.
The highest inhibition of growth rate was 2.8 % in the limit test for concentration 100 mg/L.
Therefore exact value of ErC50 could not be calculated and the value of EC are given in the form of a range.
ErC50 (72 hour) > 100 mg/L (nominal concentration, growth rate)

Executive summary:

Alga, Growth Inhibition test was performed according to the standard ČSN EN ISO 8692 (2012), Kvalita vod – Zkouška inhibice růstu sladkovodních zelených řas. The mentioned testing procedure is accredited as test method T20 in the testing laboratory.

The highest inhibition of growth rate was 2.8 % in the limit test for concentration 100 mg/L.

Therefore exact value of ErC₅₀could not be calculated and the value of EC are given in the form of a range.

ErC₅₀(72 hour) > 100 mg/L (nominal concentration, growth rate)