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EC number: 700-408-5 | CAS number: 103429-90-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2005-02-03 to 2005-03-7
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 C (Ready Biodegradability: Modified MITI Test (I))
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- other: “Method for Testing the Biodegradability of Chemical Substances by Microorganisms” stipulated in the “Testing Methods for new Chemical Substances” (November 21, 2003, Yakushokuhatsu No.1121002, November 13, 2003 Seikyoku No.2, Kanpokihatsu No.031121002)
- Deviations:
- no
- GLP compliance:
- yes
- Remarks:
- The test report cites Japanese and OECD GLP standards.
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge (adaptation not specified)
- Details on inoculum:
- - Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): Sludge was sampled from a total of ten Japanese sites; 4 sewage treatment plants, three river locations, and three lake / sea bay locations.
- Method of cultivation: Filtrate of the activated sludge was cultivated for c.a. 3 months was mixed with the filtrate of a mixture of the sludge collected from each of the 10 locations. The mixed filtrate was aerated and then the pH of the mixture was adjusted to pH 7.0 ± 1.0.
- Storage length: Sludge kept for 18 - 24 hours after the addition of synthetic water was used.
- Concentration of sludge: 3920 mg/L suspended solids. - Duration of test (contact time):
- 28 d
- Initial conc.:
- 100 mg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- TEST CONDITIONS
- Test temperature: 25±1°C.
- pH: 7.0
- pH adjusted: pH adjustment was carried out on the inoculum, prior to addition of the test material.
- Suspended solids concentration: 30 mg/L suspended solids.
- Continuous darkness: yes
TEST SYSTEM
- Culturing apparatus: 300 mL vessels in a temperature controlled bath. Each test solution was stirred by a magnetic stirrer.
- Number of culture flasks/concentration: 3
- Measuring equipment: The change in Biochemical Oxygen Demand (BOD) of the test solution was measured and recorded using a data processing machine
- Details of trap for CO2 and volatile organics if used: Soda lime
SAMPLING
- Sampling frequency: Automatic measurement of BOD
CONTROL AND BLANK SYSTEM
- Inoculum blank: Yes
- Abiotic sterile control: No
- Toxicity control: Yes - Reference substance:
- aniline
- Key result
- Parameter:
- % degradation (O2 consumption)
- Remarks:
- (Biochemical Oxygen Demand, BOD)
- Value:
- 90
- Sampling time:
- 28 d
- Key result
- Parameter:
- % degradation (DOC removal)
- Value:
- 97
- Sampling time:
- 28 d
- Key result
- Parameter:
- % degradation (test mat. analysis)
- Value:
- 100
- Sampling time:
- 28 d
- Remarks on result:
- other: Determination of test substance in test solutions at the end of the biodegradation test by GC analysis.
- Results with reference substance:
- Aniline was found to be 67% degraded by day 7, and 71% degraded by day 14, meeting the acceptability criteria for both of these time points.
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- readily biodegradable
- Conclusions:
- The test substance was readily biodegraded by microorganisms under the present test conditions.
- Executive summary:
A biodegradability study was conducted on the test material (3 -methoxy-3 -methybutyl acetate) to assess its degradability by sewage sludge. The study was conducted according to OECD 301C (Modified MITI Test) and in compliance with GLP. A solution of the test material was incubated over 28 days with an inoculum prepared with activated sludge derived from sewage treatment plants, rivers, lakes and marine sources. Degradation was monitored by analysis of Biochemical Oxygen Demand (BOD). The concentrations of the test material and two hydrolysis products were also determined at the end of the test by chromatographic techniques. The biodegradation of the test substance started before day 7 and on day 14 a mean biodegradation of 62% was reached. The test substance reached within 10 days a biodegradation of > 60% and was ultimately biodegraded with 90% at day 28.
Under the present test conditions the test substance is therefore considered readily biodegradable.
With this positive result the test substance can also be considered indicative of being rapidly and ultimately degraded in the environment which includes sewage treatment plants.
Reference
Table 1: Percentage biodegradation of the test substance
Vessel no. |
Day 7 (%) |
Day 14 (%) |
Day 21 (%) |
Day 28 (%) |
Mean degradation (%) |
1 |
- |
- |
- |
- |
|
2 |
20 |
77 |
87 |
91 |
90 |
3 |
21 |
81 |
88 |
91 |
|
4 |
20 |
29 |
67 |
88 |
|
5 |
- |
- |
- |
- |
|
6 |
67 |
71 |
76 |
79 |
|
Vessel no.:
(1) Water + test substance
(2, 3, 4) Sludge + test substance
(5) Control blank
(6) Sludge + aniline
Description of key information
The test substance is considered readily biodegradable under the conditions of the test.
Key value for chemical safety assessment
- Biodegradation in water:
- readily biodegradable
- Type of water:
- freshwater
Additional information
A biodegradability study was conducted on the test material (3 -methoxy-3 -methybutyl acetate) to assess its degradability by sewage sludge. The study was conducted according to OECD 301C (Modified MITI Test) and in compliance with GLP. A solution of the test material was incubated over 28 days with an inoculum prepared with activated sludge derived from sewage treatment plants, rivers, lakes and marine sources. Degradation was monitored by analysis of Biochemical Oxygen Demand (BOD). The concentrations of the test material and two hydrolysis products were also determined at the end of the test by chromatographic techniques. The biodegradation of the test substance started before day 7 and on day 14 a mean biodegradation of 62% was reached. The test substance reached within 10 days a biodegradation of > 60% and was ultimately biodegraded with 90% at day 28.
Under the present test conditions the test substance is therefore considered readily biodegradable.
With this positive result the test substance can also be considered indicative of being rapidly and ultimately degraded in the environment which includes sewage treatment plants.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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