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EC number: 201-972-0 | CAS number: 90-17-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Skin sensitisation
Administrative data
- Endpoint:
- skin sensitisation: in vivo (non-LLNA)
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- data from handbook or collection of data
- Justification for type of information:
- Data is from experimental report
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 980
- Report date:
- 1980
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: as mentioned below
- Principles of method if other than guideline:
- Skin sensitization study of test chemical was conducted in 10 guinea pigs to determine its sensitization potential by using Magnusson and Kligman guinea pig maximization test.
- GLP compliance:
- not specified
- Type of study:
- guinea pig maximisation test
- Justification for non-LLNA method:
- Not specified
Test material
- Reference substance name:
- 2,2,2-trichloro-1-phenylethyl acetate
- EC Number:
- 201-972-0
- EC Name:
- 2,2,2-trichloro-1-phenylethyl acetate
- Cas Number:
- 90-17-5
- Molecular formula:
- C10H9Cl3O2
- IUPAC Name:
- 2,2,2-trichloro-1-phenylethyl acetate
- Details on test material:
- - Name of test material: 2, 2, 2-trichloro-1-phenylethyl acetate (Rosacetone)
- Molecular formula: C10H9Cl3O2
- Molecular weight: 267.54
- Substance type: Organic
- Physical state: Solid
Constituent 1
In vivo test system
Test animals
- Species:
- guinea pig
- Strain:
- not specified
- Sex:
- male/female
- Details on test animals and environmental conditions:
- The weight of test animal are taken on friday of each week of the test.
Study design: in vivo (non-LLNA)
Inductionopen allclose all
- Route:
- intradermal
- Vehicle:
- other: Acetone,PEG 400, Dobs/saline
- Concentration / amount:
- Test concentration:-
•Induction (intradermal injection) : 0.5%
•0.1ml intradermal injections are made within a 2x4cm area of the shoulder region in following
sequence:-
1) 2 injections of 50% F.C.A in the solvent system chosen for the test sample.
2) 2 injections of test material at the concentration and in the solvent system chosen in the preliminary irritation tests. These injections are given into the periphery of the "bleb" caused by injection1.
3) 2 injections of test material of the concentration chosen in the preliminary irritation tests in a 50/50 mixture of F.C.A and the chosen solvent system. - Day(s)/duration:
- 7 days
- Adequacy of induction:
- other: The concentration giving definite irritation (+/-) was selected for shoulder induction
- Route:
- epicutaneous, occlusive
- Vehicle:
- other: acetone
- Concentration / amount:
- 40%
- Day(s)/duration:
- 48
- Adequacy of induction:
- other: The concentration giving definite irritation (+/-) was selected for shoulder induction.
Challengeopen allclose all
- No.:
- #1
- Route:
- epicutaneous, occlusive
- Vehicle:
- other: acetone
- Concentration / amount:
- 10%
- Day(s)/duration:
- 48 hours
- Adequacy of challenge:
- highest non-irritant concentration
- No.:
- #2
- Route:
- epicutaneous, occlusive
- Vehicle:
- other: Acetone
- Concentration / amount:
- 10%
- Day(s)/duration:
- 48 hours
- Adequacy of challenge:
- highest non-irritant concentration
- No. of animals per dose:
- 10 guinea pig / group
- Details on study design:
- RANGE FINDING TESTS: The preliminary irritation tests were performed in 4 guinea pigs to determine concentrations suitable for sensitization test [induction and challenge].As a result of the preliminary studies, the concentration chosen for skin sensitization test were 0.5% for the intradermal induction and 40% and 10% for the topical induction(neck), flank challenge respectively.
MAIN STUDY
A.I. INDUCTION EXPOSURE: Intradermal
- No. of exposures: 1
- Exposure period: 7 days
- Test groups:10 guinea pigs
- Control group: No Data Available
- Site: Shoulder region
- Frequency of applications:
- Duration: 7 days
- Concentrations: 0.5%
II. A. INDUCTION EXPOSURE: TOPICAL
- No. of exposures: 1
- Exposure period: 48 hours
- Test groups:10 guinea pigs
- Control group: No Data Available
- Site: Shoulder region
- Frequency of applications: 1
- Duration: 48 hours
- Concentrations: 40%
B. CHALLENGE EXPOSURE: Epicutaneous
- No. of exposures:1
- Day(s) of challenge: Fourteen days later, challenge test was performed
- Exposure period:24 hours
- Test groups:10guinea pigs
- Control group: :4 guinea pigs
- Site: onto the shaved flank i
- Concentrations: 10%
- Evaluation (hr after challenge): after 24 and 48 hours
C. RECHALLENGE EXPOSURE
- No. of exposures:1
- Day(s) of challenge: 1 week Later , rechalleange test was performed.
- Exposure period: No Data Available
- Test groups:10 animal
- Control group: 4 animal (same sex)
- Site: on opposite flanks
- Concentrations: 10%
- Evaluation (hr after challenge): 48 hours - Challenge controls:
- yes , concurrent vehicles were used
- Positive control substance(s):
- not specified
Results and discussion
In vivo (non-LLNA)
Resultsopen allclose all
- Reading:
- 1st reading
- Hours after challenge:
- 48
- Group:
- test chemical
- Dose level:
- 10%
- No. with + reactions:
- 0
- Total no. in group:
- 10
- Clinical observations:
- No skin sensitizing reactions were observed.
- Remarks on result:
- no indication of skin sensitisation
- Reading:
- rechallenge
- Hours after challenge:
- 48
- Group:
- test chemical
- Dose level:
- 10%
- No. with + reactions:
- 0
- Total no. in group:
- 10
- Clinical observations:
- None of the guinea pigs had showed positive results when re-challenged at concentration of 10% in acetone
- Remarks on result:
- no indication of skin sensitisation
Any other information on results incl. tables
Table 3: Results of challenge 1
Guinea pigs |
10% |
|
|
|
|
||||
No. |
Ear no. |
Sex |
Wt. (g) |
24hr |
48hr |
24hr |
48hr |
24hr |
48hr |
1 |
12574 |
M |
506 |
0 |
0 |
|
|
|
|
2 |
12622 |
M |
470 |
0 |
0 |
|
|
|
|
3 |
12538 |
M |
451 |
0 |
0 |
|
|
|
|
4 |
1254 |
M |
507 |
± |
0 |
|
|
|
|
5 |
12587 |
M |
468 |
0 |
0 |
|
|
|
|
6 |
12573 |
M |
504 |
0 |
0 |
|
|
|
|
7 |
12607 |
F |
449 |
0 |
0 |
|
|
|
|
8 |
12645 |
F |
408 |
0 |
0 |
|
|
|
|
9 |
w 12 |
F |
450 |
± |
± |
|
|
|
|
10 |
12124 |
F |
413 |
0 |
0 |
|
|
|
|
No. of positive reactions |
0 |
0 |
|
|
|
|
0=no reaction
+=barely perceptible erythema
±=Scattered, mild erythema (faint pink)
++=moderate and diffuse erythema (pale pink)
+++=intense erythema (deep pink) and odema
II) Untreated controls
1 |
12252 |
F |
420 |
0 |
0 |
|
|
|
|
2 |
0859 |
F |
444 |
0 |
0 |
|
|
|
|
3 |
12432 |
F |
502 |
0 |
0 |
|
|
|
|
4 |
-393 |
F |
406 |
0 |
0 |
|
|
|
|
5 |
|
|
|
|
|
|
|
|
|
6 |
|
|
|
|
|
|
|
|
|
7 |
|
|
|
|
|
|
|
|
|
8 |
|
|
|
|
|
|
|
|
|
9 |
|
|
|
|
|
|
|
|
|
10 |
|
|
|
|
|
|
|
|
|
Table 4: Results of challenge 2
Guinea pigs |
10% |
|
|
|
|
||||
No. |
Ear no. |
Sex |
Wt. (g) |
24hr |
48hr |
24hr |
48hr |
24hr |
48hr |
1 |
12574 |
M |
524 |
0 |
0 |
|
|
|
|
2 |
12622 |
M |
576 |
0 |
0 |
|
|
|
|
3 |
12538 |
M |
486 |
0 |
0 |
|
|
|
|
4 |
1254 |
M |
51* |
0 |
0 |
|
|
|
|
5 |
12587 |
M |
566 |
0 |
0 |
|
|
|
|
6 |
12573 |
M |
530 |
0 |
0 |
|
|
|
|
7 |
12607 |
F |
456 |
0 |
± |
|
|
|
|
8 |
12645 |
F |
518 |
0 |
0 |
|
|
|
|
9 |
w 12 |
F |
496 |
0 |
0 |
|
|
|
|
10 |
12124 |
F |
460 |
± |
0 |
|
|
|
|
II) Untreated controls
1 |
21872 |
F |
394 |
0 |
0 |
|
|
|
|
2 |
12755 |
F |
408 |
± |
± |
|
|
|
|
3 |
12818 |
F |
432 |
0 |
0 |
|
|
|
|
4 |
21873 |
F |
404 |
0 |
± |
|
|
|
|
5 |
|
|
|
|
|
|
|
|
|
6 |
|
|
|
|
|
|
|
|
|
7 |
|
|
|
|
|
|
|
|
|
8 |
|
|
|
|
|
|
|
|
|
9 |
|
|
|
|
|
|
|
|
|
10 |
|
|
|
|
|
|
|
|
|
No. of positive reactions |
0 |
0 |
|
|
|
|
Applicant's summary and conclusion
- Interpretation of results:
- other: not sensitising
- Conclusions:
- None of the guinea pigs had showed positive results when re-challenged at concentration of 10% in acetone. Thus, it can be concluded that thetest chemicalwas considered to be not sensitizing on skin ofguinea pigs.
- Executive summary:
Skin sensitization study of test chemical was conducted in 10 guinea pigs to determine its sensitization potential by using Magnusson and Kligman guinea pig maximization test.
The preliminary irritation tests were performed in 4 guinea pigs to determine concentrations suitable for sensitization study[induction and challenge].As a result of the preliminary studies, the concentrationchosen forskin sensitization test were0.5% for the intradermal induction and 40% and 10% for the topical induction(neck), flank challenge respectively.
During the sensitization test, 10 untreated guinea pigs were given, six 0.1ml intradermal injections are made within a 2x4cm area of the shoulder. 7 Days later sensitization was boosted by placing over the shoulder injection site a 2x4cm filter paper patch saturated with 40% in acetone. The patch test occluded with thin polythene, held in place by poroplast, and was left in place for 48 hours.
14 days after application of the shoulder patch, challenge exposure was carried out on one flank of the guinea pigs by an occlusive patch. The cup was applied to shaved flank and held in place by Poroplast wound around the trunk. 24 hours later the patch was removed, the reaction being scored at 24, 48 hours subsequently. One week later, the challenge exposure was repeated on the other flank
None of the guinea pigs had showed positive results when re-challenged at concentration of 10% in acetone. Thus, it can be concluded that thetest chemicalwas considered to be not sensitizing on skin of guinea pigs.
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