2,2,2-trichloro-1-phenylethyl acetate

Eye irritation

Administrative data

Endpoint:

eye irritation: in vivo

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

data from handbook or collection of data

Justification for type of information:

Data is from experimental report

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1979

Report date:

1979

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: Modified Federal Hazardous Labelling Act

Principles of method if other than guideline:

The test was designed to investigate the irritation effects of test chemical when applied to the one eye of single rabbits.

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

2,2,2-trichloro-1-phenylethyl acetate

EC Number:

201-972-0

EC Name:

2,2,2-trichloro-1-phenylethyl acetate

Cas Number:

90-17-5

Molecular formula:

C10H9Cl3O2

IUPAC Name:

2,2,2-trichloro-1-phenylethyl acetate

Details on test material:

- Name of test material (as cited in study report): 2,2,2-trichloro-1-phenylethyl acetate (rosacetone)
- Substance type: Organic
- Physical state: Solid

Test animals / tissue source

Species:

rabbit

Strain:

New Zealand White

Details on test animals or tissues and environmental conditions:

- Age at study initiation: aged 7-9 weeks
- Weight at study initiation:weighing between 1-2 Kg

Test system

Vehicle:

unchanged (no vehicle)

Controls:

not specified

Amount / concentration applied:

10 mg

Duration of treatment / exposure:

24 hrs.

Observation period (in vivo):

15 min. after application
24 hrs. after application
daily intervals for 5 days per week for 3 weeks

Number of animals or in vitro replicates:

6 animals

Details on study design:

The lower eyelid is gently pulled away from the globe and 10 mg of test substance applied into the cup so formed ;the eyelid then release. The rabbit was inspected with hand torch before any treatment and those with spontaneous eye lesions are rejected.Flurescein is applied to eye to assist in the detection of corneal lesions.Corneal thickness is also measured before the application ot test material

Results and discussion

In vivo

Results

Irritation parameter:

overall irritation score

Basis:

mean

Time point:

24/48/72 h

Reversibility:

other: Not applicable

Remarks on result:

no indication of irritation

Irritant / corrosive response data:

The test chemical caused no corneal, conjunctival, or iridial damage 24 hours after treatment.

Any other information on results incl. tables

Table 1: Summary of test result

Experimental group	No of rabbits	Discomfort	Number of rabbits with stated ocular lesion
			Positive conjunctival reactions	No. with iritis	No. with pannus	Maximum corneal opacity					Duration of opacity		Maximum corneal swelling
						0.5	1	2	3	4	>1 on day 8	>0.5 on day 8	Nil (0-10%)	Slight (11-45%)	Moderate (46-80%)	Severe (81%+)
A	1	-	-	-	-	-	-	-	-	-	-	-	1	-	-	-

Applicant's summary and conclusion

Interpretation of results:

not irritating

Conclusions:

The test chemical caused no corneal, conjunctival, or iridial damage 24 hours after treatment at concentration of 10mg. Hence, the test material can be considered to be not irritating to New Zealand White rabbit eyes.

Executive summary:

An eye irritation study was conducted in accordance with Modified Federal Hazardous Labelling Act Method by using a single rabbit.

The rabbit was inspected with a hand torch before any treatment and those without any serious corneal lesions were taken. Corneal thickness was also measured before the start of the test. The lower eyelid was gently pulled away from the globe and 10 mg of test substance applied on one eye into the cup so formed; the eyelid was then released.

Animal was watched during and immediately after the application of the test chemical. 15 minutes after the application of the test chemical the eyes were observed for conjunctival reactions. One day after the application of test material and then at daily intervals for five days per week for three weeks or until the eye is completely clear. Fluorescein was applied to eyes to assist in the detection of corneal lesions.

Each day after application of the test chemical, the corneal thickness was measured using a slit lamp. Corneal swelling was expressed as:

(Corneal thickness on day of measurement)/(Corneal thickness on before treatment)-1)*100%.

The test chemical caused no corneal, conjunctival, or iridial damage 24 hours after treatment at tested concentration of 10 mg.

Hence, the test chemical can be considered to be not irritating to New Zealand White rabbit eyes.