Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 252-328-0 | CAS number: 35037-73-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- OCT 2019 - AUG 2020
- Reliability:
- 1 (reliable without restriction)
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Deviations:
- no
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- For the registered substance 4-trifluoromethoxy phenyl isocyanate (CAS No. 35037-73-1), the water
molecules will react with free isocyanate groups to generate carbon dioxide and primary amines
which forms 4-trifluoromethoxy aniline (CAS No. 461-82-5), that is a hydrolysis product of 4-
trifluoromethoxy phenyl isocyanate. Thus, in this endpoint the hydrolysis product is tested. For details of hydrolysis process please refer to section 13 "expert statement". - Analytical monitoring:
- yes
- Details on sampling:
- A stock solution was prepared to give the desired series of test concentrations. 100.9 mg of the test item were added to a 1 litre portion of dilution water and stirred for 0.5 h on a magnetic stirrer. The solution was apparently solved and was clear without visible particles (no Tyndall-effect was observed). The pH was measured to be 7.8.
- Vehicle:
- no
- Details on test solutions:
- To produce the different test item concentrations appropriate amounts of the stock solution were diluted with dilution water to a volume of 500 mL. 50 mL of the prepared solutions were taken per replicate and 0.5 mL of dilution water containing 5 daphnids was given to all replicates resulting in the final nominal concentrations. For each test item exposure concentration and for the control exposure 4 replicates were prepared.
- Test organisms (species):
- Daphnia magna
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Hardness:
- 14.4 °dH (= 257 mg/L CaCO3)
- Test temperature:
- 18 - 22 °C
- Details on test conditions:
- The main test was conducted as a static test with the test medium unchanged throughout the duration of the test.
During the test a temperature range of 18 - 22 °C was maintained in the test vessels, with a maximum temperature fluctuation of +/- 1 °C in each individual test.
The temperature, the pH and the oxygen values were measured at the beginning and end of the test. In order to avoid any potential impairment of the test system, an additional replicate was used at the beginning of the test. Measurements at the end of the test were performed using replicate 1 of the test concentrations and the control vessels. - Reference substance (positive control):
- yes
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- 7.3 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 0.67 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Validity criteria fulfilled:
- yes
- Conclusions:
- The EC50 after 24 h was 7.3 mg/L. The EC50 after 48 h was 0.67 mg/L.
- Executive summary:
The short-term toxicity of 4-trifluoromethoxyaniline towards aquatic invertebrates was tested according to OECD Guideline 202 and under GLP. The daphnids (Daphnia magna) were exposed to a range of concentrations, nominally 0.018, 0.040, 0.088, 0.19, 0.43, 0.94, 2.1, 4.5 and 10 mg/L of 4-trifluoromethoxyaniline dissolved in dilution water. Auxiliaries used to prepare the test media was an magnetic stirrer.
Observations were made on the swimming ability and the percentage immobilised, respectively, after 24 and 48 hours of exposure. The following values were determined: EC50 (24h): 7.3 mg/L and EC50 (48h): 0.67 mg/L.
Reference
Description of key information
Short term toxicity to aquatic invertebrate, 2020:OECD 202, EU Method C.2; Daphnia magna; EC50 (48h): 0.67 mg/L
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Dose descriptor:
- EC50
- Effect concentration:
- 0.67 mg/L
Additional information
The hydrolysis product of TFMOPI, Trifluormethoxyaniline, was tested in an acute toxicity test with Daphnia magna for 24h, resulting an EC50 of 19.6 mg/L.
Isocyanates are known to rapidly hydrolyse under the formation of the corresponding amine as well as solid polyurea compounds *. However, the insoluble inert polyurea is not assumed to cause environmental hazards due to the reduced uptake of high molecular mass compounds, whereas the amine is assumed to be the ecotoxicological relevant species. Concluding, tests performed using the corresponding amine can equally be used to assess the ecotoxicological hazards of isocyanates assuming the complete transfer of the isocyanate into the corresponding amine while neglecting the formation of a certain fraction ecotoxicological not active polyurea compounds. An underestimation of environmental hazards is thus avoided supporting a conservative and thus protective hazard assessment.
* Bayer Industry Services (2004). 4-chlorophenyl-isocyanate: Investigation on Stability in Aqueous Test Solutions. Project No: 200300181. Leverkusen, Germany.
Bayer Industry Services (2003). Isopropylisocyante: Investigation on Stability in Aqueous Test Solutions. Project No: 200300095. Leverkusen, Germany.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.