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EC number: 203-402-6 | CAS number: 106-48-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1988
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Guideline study. All relevant validity criteria were met with acceptable deviations.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Deviations:
- yes
- Remarks:
- No positive control conducted as part of the study.
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- EU Method C.1 (Acute Toxicity for Fish)
- Deviations:
- yes
- Remarks:
- No positive control conducted as part of the study.
- GLP compliance:
- not specified
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: 0 (control), to 1000 mg/L
Time Weighted Mean Measured equivalent concentrations:
- Sampling method: Water samples were taken from the control and all surviving test groups at 0 (fresh media); 24, 48, 72 (old and fresh media) and 96 (old media) hours for quantitative analysis. Samples were prepared for analysis on the day of sampling.
- Sample storage conditions before analysis: See above. - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Direct dissolution of test item in DMSO to prepare stock; serial dilution of stock in culture medium to prepare test media. The test concentrations used in the definitive study were prepared by adding an amount of test item to the vortex created in a volume of water to give an initial dispersion at a concentration of 1000 mg/L. Stirring periods of 24 hours and 96 hours were employed and samples taken for chemical analysis. Based on the results of the range-finding study the test item solutions for the definitive study were prepared by stirring an excess of test item (equivalent to 1000 mg/L) in culture medium.
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): DMSO used due to low solubility of test item in aqueous solution.
- Concentration of vehicle in test medium (stock solution and final test solution): Not applicable.
- Evidence of undissolved material (e.g. precipitate, surface film, etc): No precipitate reported. - Test organisms (species):
- Oryzias latipes
- Details on test organisms:
- TEST ORGANISM
- Common name: Japanese Medaka / Japanese Rice Fish (Oryzias latipes)
- Strain: Not reported.
- Source: Recognised supplier.
- Age at study initiation (mean and range, SD): Not reported.
- Length at study initiation (length definition, mean, range and SD): 2.0 cm
- Weight at study initiation (mean and range, SD): 0.2 g
- Method of breeding: Not reported.
- Maintenance of the brood fish: Not reported.
ACCLIMATION
- Acclimation period: 15 days.
- Acclimation conditions (same as test or not): Yes.
- Type and amount of food during acclimation: Not reported.
- Feeding frequency during acclimation: daily (until 24 hours prior to test).
- Health during acclimation (any mortality observed): No mortality reported prior to test.
QUARANTINE (wild caught)
- Duration: Not applicable.
- Health/mortality: Not applicable.
FEEDING DURING TEST
- Food type: Not applicable.
- Amount: Not applicable.
- Frequency: Not applicable. - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Remarks on exposure duration:
- According to OECD TG 203 guidelines.
- Post exposure observation period:
- none.
- Hardness:
- ca. 50-70 mg/L (expressed as CaCO3)
- Test temperature:
- 25 +/- 1 ºC
- pH:
- 7.7 - 7.8 (fresh media)
- Dissolved oxygen:
- Not reported.
- Salinity:
- not applicable.
- Nominal and measured concentrations:
- Concentrations: 0 (control), to 1000 mg/L analytically confirmed initial concentration.
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 15 L for test and control vessels, supplied by Tetra Werke.
- Type (delete if not applicable): Not specified.
- Material, size, headspace, fill volume: 15L fill volume test media per test vessel
- Aeration: Not specified.
- Renewal rate of test solution (frequency): 24 h
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- No. of vessels per vehicle control (replicates): Not applicable
- Biomass loading rate: Not specified.
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Laboratory water supply.
- Total organic carbon: Not reported
- Particulate matter: Not reported
- Metals: Not reported
- Pesticides: Not reported
- Chlorine: Not reported
- Alkalinity: Not reported
- Ca/mg ratio: Not reported
- Conductivity: Not reported
- Culture medium different from test medium: No.
- Intervals of water quality measurement: 24 hours.
OTHER TEST CONDITIONS
- Adjustment of pH: No.
- Photoperiod: 16 h light / 8 h darkness
- Light intensity: 1500 lux.
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : mortality and visible abnormality recorded at 3 h, 24 h, 48 h, 72 h and 96 h.
TEST CONCENTRATIONS
- Spacing factor for test concentrations: Not applicable. Concentrations justified from the results of the range finding study.
- Range finding study: yes (semi-static) ; 3 organisms per concentration.
- Test concentrations: blank control, 0-1000 mg/L nominal
- Results used to determine the conditions for the definitive study: Yes. - Reference substance (positive control):
- no
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 4.9 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: (95% CI: 3.7 - 6.6 mg/L) ; time weighted mean measured concentrations
- Details on results:
- - Behavioural abnormalities: Not reported.
- Observations on body length and weight: Not reported
- Other biological observations: Not reported
- Mortality of control: 0%
- Other adverse effects control: No abnormalities observed in the control.
- Abnormal responses: Not reported.
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: Not reported.
- Effect concentrations exceeding solubility of substance in test medium: Not applicable - Validity criteria fulfilled:
- yes
- Conclusions:
- The 96 hour LC50 for the test item to Japanese Medaka (Oryzias latipes) was determined to be 4.9 (C.I. 3.7 - 6.6) mg/L based on time weighted mean measured concentrations.
- Executive summary:
The acute toxicity of the test item to Japanese Medaka (Oryzias latipes) was determined in a 96 hour semi-static test according to OECD TG 203, equivalent to EU Method C.1 guidelines. Following a preliminary range-finding test, fish were exposed, in groups often, to an aqueous solution of the test item at time-weighted mean measured test concentrations of 0 -1000 mg/L for a period of 96 hours under semi-static test conditions. Based on the results of the range-finding study the test item solutions for the definitive study were prepared by stirring an excess of test item (equivalent to 1000 mg/L) in culture medium for a period of time, to produce the test concentrations. The effect levels were estimated based on time weighted mean measured concentrations. Under the conditions of this study, the 96h LC50 was 4.9 (C.I. 3.7 – 6.6) mg/L based on time weighted mean measured concentrations.
Reference
Description of key information
LC50 (fish) = 4.9 (C.I. 3.7 – 6.6) mg/L based on time weighted mean measured concentrations, 96hour, freshwater, OECD TG 203, 1988
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 4.9 mg/L
Additional information
Key study : OECD TG 201, 2000 : The acute toxicity of the test item to Japanese Medaka (Oryzias latipes) was determined in a 96 hour semi-static test according to OECD TG 203, equivalent to EU Method C.1 guidelines. Following a preliminary range-finding test, fish were exposed, in groups often, to an aqueous solution of the test item at time-weighted mean measured test concentrations of 0 -1000 mg/L for a period of 96 hours under semi-static test conditions. Based on the results of the range-finding study the test item solutions for the definitive study were prepared by stirring an excess of test item (equivalent to 1000 mg/L) in culture medium for a period of time, to produce the test concentrations. The effect levels were estimated based on time weighted mean measured concentrations. Under the conditions of this study, the 96h LC50 was 4.9 (C.I. 3.7 – 6.6) mg/L based on time weighted mean measured concentrations.
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