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EC number: 605-140-1 | CAS number: 158237-07-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to terrestrial arthropods
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to terrestrial arthropods: short-term
- Type of information:
- experimental study
- Adequacy of study:
- other information
- Study period:
- 11 - 16 Apr 1997
- Reliability:
- 4 (not assignable)
- Rationale for reliability incl. deficiencies:
- documentation insufficient for assessment
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 214 (Honeybees, Acute Contact Toxicity Test)
- GLP compliance:
- no
- Application method:
- contact
- Analytical monitoring:
- not required
- Vehicle:
- yes
- Details on preparation and application of test substrate:
- - Method of test material application: single topical dose
- Body part: dorsal-thorax
- Volume of test solution applied: 1 µL
- Controls: yes
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): acetone - Test organisms (species):
- Apis mellifera
- Animal group:
- Hymenoptera (honeybees)
- Details on test organisms:
- TEST ORGANISM
- Common name: Honeybee
- Source: Purchased from Nonogaki Bee Farm in Aichi prefecture, in February 1996
- Age at test initiation (mean and range, SD): Workers after emergence 4 to 7 days were used
- Stage at test initiation: workers
- Cultural background (if honeybees): Hybrid colony for pollination - Study type:
- laboratory study
- Limit test:
- no
- Total exposure duration:
- 48 h
- Test temperature:
- 32 °C
- Details on test conditions:
- TEST SYSTEM
- Test container / cage (material, size): plastic cups (RISU PACK®, diameter: 101 mm, hight: 45 mm, volume: 238 mL)
- No. of organisms per container (treatment): 10
- No. of replicates per treatment group: 3
- No. of replicates per control / vehicle control: 3
- No. of trials: 2
- Food: fed with honey during exposure
EFFECT PARAMETERS MEASURED (with observation intervals if applicable): After 24 and 48 hours, number of dead bees was counted.
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 1.5 to 2
- Test item was dissolved in acetone and diluted to the designed concentrations. - Nominal and measured concentrations:
- control, 12.5, 25, 50, 100, and 150 µg/animal (nominal)
- Reference substance (positive control):
- no
- Duration:
- 48 h
- Dose descriptor:
- LD50
- Effect conc.:
- > 150 µg per animal
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 150 µg per animal
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Endpoint:
- toxicity to terrestrial arthropods: short-term
- Type of information:
- experimental study
- Adequacy of study:
- other information
- Study period:
- 11 - 16 Apr 1997
- Reliability:
- 4 (not assignable)
- Rationale for reliability incl. deficiencies:
- documentation insufficient for assessment
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 214 (Honeybees, Acute Contact Toxicity Test)
- GLP compliance:
- no
- Application method:
- contact
- Analytical monitoring:
- not required
- Vehicle:
- yes
- Details on preparation and application of test substrate:
- - Method of test material application: single topical dose
- Body part: dorsal-thorax
- Volume of test solution applied: 1 µL
- Controls: yes
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): acetone - Test organisms (species):
- Apis mellifera
- Animal group:
- Hymenoptera (honeybees)
- Details on test organisms:
- TEST ORGANISM
- Common name: Honeybee
- Source: Purchased from Nonogaki Bee Farm in Aichi prefecture, in February 1996
- Age at test initiation (mean and range, SD): Workers after emergence 4 to 7 days were used
- Stage at test initiation: workers
- Cultural background (if honeybees): practical hybrid for mating - Study type:
- laboratory study
- Limit test:
- no
- Total exposure duration:
- 48 h
- Test temperature:
- 32 °C
- Details on test conditions:
- TEST SYSTEM
- Test container / cage (material, size): plastic cups (RISU PACK®, diameter: 101 mm, hight: 45 mm, volume: 238 mL
- No. of organisms per container (treatment): 10
- No. of replicates per treatment group: 3
- No. of replicates per control / vehicle control: 3
- No. of trials: 2
- Food: fed with honey during exposure (placed in a centrifugal pipe, which was inserted into the cover of the container cup)
EFFECT PARAMETERS MEASURED (with observation intervals if applicable): After 24 and 48 hours, number of dead bees was counted
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 1.5 to 2
- Test item was dissolved in acetone and diluted to the designed concentrations. - Nominal and measured concentrations:
- control, 12.5, 25, 50, 100, and 150 µg/animal (nominal)
- Reference substance (positive control):
- no
- Duration:
- 48 h
- Dose descriptor:
- LD50
- Effect conc.:
- > 150 µg per animal
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 150 µg per animal
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
Referenceopen allclose all
Table 1: Effect of the test substance against honeybee with topical application method; Trial 1
|
Dose [µg/animal] |
No. of bees |
Number of dead bees |
Mortality [%] |
|
24 hrs |
48 hrs |
||||
Test substance |
150 |
30 |
0 |
0 |
0 |
100 |
30 |
0 |
0 |
0 |
|
50 |
30 |
0 |
0 |
0 |
|
25 |
30 |
0 |
0 |
0 |
|
12.5 |
30 |
0 |
0 |
0 |
|
Control |
- |
30 |
0 |
0 |
0 |
Table 2: Effect of the test substance against honeybee with topical application method; Trial 2
|
Dose [µg/animal] |
No. of bees |
Number of dead bees |
Mortality [%] |
|
24 hrs |
48 hrs |
||||
Test substance |
150 |
30 |
0 |
0 |
0 |
100 |
30 |
0 |
0 |
0 |
|
50 |
30 |
0 |
0 |
0 |
|
25 |
30 |
0 |
0 |
0 |
|
12.5 |
30 |
0 |
0 |
0 |
|
Control |
- |
30 |
0 |
0 |
0 |
Table 1: Effect of the test substance against honeybee with topical application method; Trial 1
|
Dose [µg/animal] |
No. of bees |
Number of dead bees |
Mortality [%] |
|
24 hrs |
48 hrs |
||||
Test substance |
150 |
30 |
0 |
0 |
0 |
100 |
30 |
0 |
0 |
0 |
|
50 |
30 |
0 |
0 |
0 |
|
25 |
30 |
0 |
0 |
0 |
|
12.5 |
30 |
0 |
0 |
0 |
|
Control |
- |
30 |
0 |
0 |
0 |
Table 2: Effect of the test substance against honeybee with topical application method; Trial 2
|
Dose [µg/animal] |
No. of bees |
Number of dead bees |
Mortality [%] |
|
24 hrs |
48 hrs |
||||
Test substance |
150 |
30 |
0 |
0 |
0 |
100 |
30 |
0 |
0 |
0 |
|
50 |
30 |
0 |
0 |
0 |
|
25 |
30 |
0 |
0 |
0 |
|
12.5 |
30 |
0 |
0 |
0 |
|
Control |
- |
30 |
0 |
0 |
0 |
Description of key information
Key value for chemical safety assessment
Additional information
In accordance with Regulation (EC) No 1907/2006, Annex IX, column 2, testing of effects on terrestrial organisms is not required since direct and indirect exposure of the soil compartment for the substance 4-(2-chlorophenyl)-N-cyclohexyl-N-ethyl-5-oxo-4,5-dihydro-1H-tetrazole-1-carboxamide is unlikely. Furthermore, the evaluation of the PEC/PNEC ratio indicates no risk for the terrestrial compartment (RCR << 1). For detailed information, see Chemical Safety Report (CSR) chapter 9 and 10.
Two studies are available (1997a, 1997b) investigating the toxicity of the test substance to terrestrial arthropods. No guideline was given in the study reports, nor were the studies conducted according to GLP, but the study procedure is comparable to OECD 214. Apis mellifera was used as test organism. Thirty bees, 10 per replicate, were exposed for 48 h to a range of test substance doses between 12.5 and 150 µg per animal. The test item was dissolved in acetone. 1 µL test substance dilution was applied by single topical application on the dorsal thorax. Two trials were conducted. No mortalities and no effects were observed in both studies. Thus, a LD50 greater than 150 µg per animal was determined.
Further data from a non-guideline, non-GLP study with silkworm larvae (Bombyx mori) is available. Serial water dilutions of the test substance at 1000 ppm and at 100 ppm with a surfactant 0.1% Neoesterin were prepared. Tested mulberry leaves were dipped in each of them. The leaves for the control group were dipped in water with surfactant. Silkworms were fed on mulberry leaves for 25 days. The living and dead larvae were counted, and the feces were weighed every day until cocoons were formed. The weight of silkworm feces indicated the amount of mulberry leaves ingested. After that, quality of cocoons was investigated. For each dosage, twenty silkworms were used without replication. No mortality and no toxic symptoms were observed in both treatments. However, growth of the silkworm in the group dosed at 1000 ppm was delayed three days compared to the control group. There was no difference in weight of silkworm feces between the 100 ppm and the control group. The weight of the feces at 1000 ppm was less compared to the control group. The quality of cocoons in the 1000 ppm and 100 ppm groups was comparable to the control group.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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