Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 947-711-0 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- from 2014-04-11 to 2014-10-22
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study with acceptable restrictions
- Qualifier:
- according to guideline
- Guideline:
- other: Guidelines for the Testing of Chemical, SEPA, P.R. China No. 203 " Fish, Acute Toxicity Test" (2004)
- Deviations:
- no
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Deviations:
- no
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Remarks:
- gas chromatography
- Details on sampling:
- The concentrations of the solutions were tested at the begining and at the end of the test.
- Vehicle:
- no
- Details on test solutions:
- WAF solutions were prepared by adding 0.0253 g, 0.0355 g, 0.0507 g, 0.0709 g and 0.1012 g of test item to 10L test medium respectively and adding 0.0759 g, 0.1063 g, 0.1517 g, 0.2124 g and 0.3033 g of test item to 30L test medium respectively, and the solutions with loading rate of 2.5 mg/L, 3.5 mg/L, 5 mg/L, 7 mg/L and 10 mg/L were obtained. The solutions were mixed for 48 h at the speed of 300 rpm using electric stirrer at 30 ± 2 °C. Then the 10L and 30L solutions were combined after mixing and a total of 40L solutions with chosen loading rate were obtained. Following cessation of mixing and settling for 4 hours, the WAFs were filtered with glass wool and the final test solutions were obtained.
- Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- TEST ORGANISM
- Common name: Zebra fish
- Length at study initiation: 2.1 to 3.0 cm
ACCLIMATION
- Acclimation period: 170 days
- Acclimation conditions: same as test
- Feeding frequency during acclimation: once a day, ceasing 24 hours before the start of the test - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Hardness:
- 10 to 250 mg CaCO3 per L
- Test temperature:
- 23 ± 2 °C
- pH:
- 8.28 - 8.31
- Dissolved oxygen:
- 5.27 - 8.58 mg/L
- Nominal and measured concentrations:
- Nominal concentrations: 2.5, 3.5, 5, 7 and 10 mg/L
Measured concentrations: 1.61, 2.15, 3.61, 4.67 and 5.52 mg/L - Details on test conditions:
- TEST SYSTEM
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 3
- Biomass loading rate: 0.25 g fish/L
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: tap water treated with an active carbon water filter, the quartz sand water filter, micro-filtration water filter, ultraviolet light and aeration
OTHER TEST CONDITIONS
- Photoperiod: 12 hours light photopcriod daily
- Light intensity: lamplight and natural light
OBSERVATIONS
- at 3, 6, 24, 48, 72 and 96 hours - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 4.1 mg/L
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Details on results:
- - Nomortality in the blank control was observed.
- Highest concentration producing 0 % mortality during test was 2.15 mg/L based on measured concentration.
- Lowest concentration producing 100 % mortality during test was 5.52 mg/L based on measured concentration. - Results with reference substance (positive control):
- The 24h LC50 value of Potassium dichromate was determined to be 248.542 mg/L.
- Sublethal observations / clinical signs:
Table 1. Toxicity symptoms and mortality (Preliminary test)
Concentration (mg/L)
Fish Number
Time (hr)
Cumulative Mortality
Abnormalities
Blank control
5
24
0
None
48
0
72
0
96
0
1.25
5
24
0
None
48
0
72
0
96
0
2.5
5
24
0
None
48
0
72
0
96
0
5
5
24
0
Death, loss of equilibrium, swimming behavior decreased
48
0
72
1
96
1
10
5
24
0
Death, loss of equilibrium
48
0
72
4
96
5
20
5
24
0
Death, loss of equilibrium
48
4
72
5
96
5
Table 2. Cumulative Mortality at Each Concentration
Concentration (mg/L)
(loading rate/measured)
Cumulative mortality (%)
24 h
48 h
72 h
96 h
2.5/1.61
0 %
0 %
0 %
0 %
3.5/2.15
0 %
0 %
0 %
0 %
5/3.61
0 %
13 %
17 %
30 %
7/4.67
0 %
33 %
33 %
63 %
10/5.52
0 %
70 %
93 %
100 %
The acute toxicity of the test item was calculated in terms of measured concentrations and the results were as follows:
24-h LC50: > 5.52 mg/L
48-h LC50: 4.968 mg/L with 95 % confidence interval of 4.617 - 5.504 mg/L
72-h LC50: 4.604 mg/L with 95 % confidence interval of 2.645 - 9.898 mg/L
96-h LC50: 4.100 mg/L with 95 % confidence interval of 3.813 - 4.348 mg/L
Table 3. Toxicity symptoms and mortality(Maintest)
Nominal Concentration (mg/L)
Fish Number
Time (hr)
Cumulative Mortality
Abnormalities
Replicate
Average
1
2
3
Blank control
30
3
0
0
0
0
None
6
0
0
0
0
24
0
0
0
0
48
0
0
0
0
72
0
0
0
0
96
0
0
0
0
2.5 mg/L
30
3
0
0
0
0
None
6
0
0
0
0
24
0
0
0
0
48
0
0
0
0
72
0
0
0
0
96
0
0
0
0
3.5 mg/L
30
3
0
0
0
0
None
6
0
0
0
0
24
0
0
0
0
48
0
0
0
0
72
0
0
0
0
96
0
0
0
0
5 mg/L
30
3
0
0
0
0
Death, loss of equilibrium, swimming behavior decreased
6
0
0
0
0
24
0
0
0
0
48
2
1
1
1.3
72
3
1
1
1.7
96
4
2
3
3
7 mg/L
30
3
0
0
0
0
Death, loss of equilibrium, swimming behavior decreased
6
0
0
0
0
24
0
0
0
0
48
4
3
3
3.3
72
4
3
3
3.3
96
7
5
7
6.3
10mg/L
30
3
0
0
0
0
Death, loss of equilibrium, swimming behavior decreased
6
0
0
0
0
24
0
0
0
0
48
7
8
6
7
72
10
9
9
9.3
96
10
10
10
10
Analytical results
According to the results of the stability test, the concentration of the test item in water could maintain within 80 - 120 % of initial. So the test was conducted with static regime and the test vessel was covered during the test.
Table 4. Concentration measurement in the main test
Nominal concentration (mg/L)
Replicate
Measured concentration 0 h (mg/L)
Measured concentration 96 h (mg/L)
2.5
1
1.57
1.88
2
1.63
1.62
3
1.63
1.45
3.5
1
2.17
2.23
2
2.13
1.86
3
2.16
1.94
5
1
3.53
3.68
2
3.61
3.00
3
3.69
3.02
7
1
4.64
4.06
2
4.73
4.42
3
4.65
4.12
10
I
5.34
5.69
2
5.78
5.86
3
5.43
5.49
- Validity criteria fulfilled:
- yes
- Conclusions:
- The toxicity of the test item on Zebrafish was determined in a 96-h study. The LC50 was determined to be 4.1 mg/L.
- Executive summary:
The acute toxicity of the test item to fish (zebrafish) was determined according to The Guidelines for the Testing of Chemical, SEPA, P.R. China No. 203 "Fish, Acute Toxicity Test" (2004)and OECD Guideline 203. A static test was performed with nominal concentrations of 2.5 mg/L, 3.5 mg/L, 5 mg/L, 7 mg/L and 10 mg/L. The test item solution was prepared by WAF method in filtered tap water. 30 test organisms in 3 replicates were exposed to the test item solutions and the control each for 96 hours. The measured concentration of the test item at the start and at the end of the exposure period was in the range 80 - 120 %. Therefore, the biological endpoints were based on the measured concentrations. The LC50 after 96 h was determined to be 4.1 mg/L.
Reference
Description of key information
The toxicity of the test item on Zebra fish was determined in a 96-h study. The LC50 was determined to be 4.1 mg/L.
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 4.1 mg/L
Additional information
The acute toxicity of the test item to fish (zebra fish) was determined according to The Guidelines for the Testing of Chemical, SEPA, P.R. China No. 203 "Fish, Acute Toxicity Test" (2004) and OECD Guideline 203. A static test was performed with nominal concentrations of 2.5 mg/L, 3.5 mg/L, 5 mg/L, 7 mg/Land 10 mg/L. The test item solution was prepared by WAF method in filtered tap water. 30 test organisms in 3 replicates were exposed to the test item solutions and the control each for 96 hours. The measured concentration of the test item at the start and at the end of the exposure period was in the range 80 - 120 %. Therefore, the biological endpoints were based on the measured concentrations. The LC50 after 96 h was determined to be 4.1 mg/L.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.