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EC number: 947-711-0 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- from 2012-05-17 to 2012-07-27
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study with acceptable restrictions
- Qualifier:
- according to guideline
- Guideline:
- other: Guidelines for the testing of chemicals, SEPA, P.R. China No. 301C "Modified MITI Test (I)" 2004
- Deviations:
- no
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 301 C (Ready Biodegradability: Modified MITI Test (I))
- Deviations:
- no
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- EU Method C.4-F (Determination of the "Ready" Biodegradability - MITI Test)
- Deviations:
- no
- GLP compliance:
- yes
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, non-adapted
- Details on inoculum:
- Samples form ten sites were collected, mainly areas where a variety of chemicals were used and discharged. The sites included sewage treatment plants. industrial wastewater treatment plants, rivers, lakes, seas. 1 L sample of sludge, surface soil, water and etc. was collected and then mixed together.
After removal of the floating matter and settling, the pH of the supernatant was measured to be 7. The supernatant was colelcted and aerated for 23.5 h. 30 minutes after the aeration was stopped, 1/3 of the supernatant was discarded. Then equal volume of solution containig 0.1 % of glucosem peptonem potassium prthophosphate was added and the aeration was continued. This procedure was repeated once a day for a month. The temperature was kept at 25 ± 1 °C and the pH at 7 ± 1. After one month the sludge was used as microbial inoculum. Before the start of the test a small amount waa dried to determined the wet weight of the sludge.
The inoculum concentration used for the test was 30 mg SS/L. - Duration of test (contact time):
- 28 d
- Initial conc.:
- 100 mg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- TOC removal
- Details on study design:
- - 1 bottle with the test item in deionised water as abiotic control
- 3 bottles with test item and inoculum
- 1 bottle with reference item and inoculum as procedure control
The test was performed in complete darkness.
The tets system was placed in an incubator at a temperature of 20 ± 1 °C.
- 1 bottle only with inoculum as inoculum control - Reference substance:
- aniline
- Key result
- Parameter:
- % degradation (TOC removal)
- Value:
- 30.85
- Sampling time:
- 28 d
- Details on results:
- The theoretical oxygen uptake of the test item was 3.09 mg O1/mg test item. Within 28-day test period, the cumulated % biodegradation of the test item was 30.85 %, % abiotic degradation was 27.27 % and % primary degradation was 7.12 % based on TOC calculation.
- Results with reference substance:
- The cumulated % biodegradation of the reference substance after 28 days was 81.74 %.
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- inherently biodegradable
- Conclusions:
- The test item showed 31 % biodegradation in a Modified MITI Test. Therefore, the test item is considered to be not readily biodegradable, but inherently biodegradable as more than 20 % biodegradation was observed.
- Executive summary:
The ready biodegradability of the test item was determined with an activated sludge over a test period of 28 days in the Modified MITI Test (I) according to OECD 301C, Regulation (EC) No. 440/2008 Method C.4-F and Guidelines for the testing of chemicals, SEPA, P.R. China No. 301C "Modified MITI Test (I)" 2004. The test item was tested at a concentration of 100 mg/L in triplicates, corresponding to carbon content of 79.25 % and theoretical oxygen uptake of 3.09 mg O2/mg test item. The total organic carbon was measured with TOC Analyzer. The biodegradation of the test item came to 30.85 % after 28 days. The reference item showed 81.74 % degradation after 28 days. In conclusion, the test item is not readily biodegradable as the pass level of 60 % degradation was not reached in a 10-d-window within the 28 days period of the study. As more than 20 % biodegradation was observed, the test item can be cosidered as inherently biodegradable.
Reference
Table 1. Biodegradation results of the test item (28 days)
Time (d) |
Cumulative BOD (mg/L) |
BOD (mg O2/mg test item) |
Cumulative percentage biodegradation (%) |
||||
Abiotic control |
Inoculum control (IC) |
Test item (TI) |
|||||
BIC |
IC |
TI-1 |
TI-2 |
TI-3 |
|||
1 |
0 |
0 |
0 |
0 |
0 |
0.00 |
0.00 |
2 |
1 |
0 |
2 |
1 |
1 |
0.01 |
0.43 |
3 |
3 |
0 |
6 |
7 |
6 |
0.06 |
2.05 |
4 |
6 |
0 |
12 |
14 |
17 |
0.14 |
4.64 |
5 |
17 |
1 |
21 |
23 |
28 |
0.23 |
7.44 |
6 |
37 |
1 |
44 |
47 |
45 |
0.44 |
14.35 |
7 |
68 |
2 |
57 |
55 |
62 |
0.56 |
18.12 |
8 |
70 |
3 |
69 |
68 |
74 |
0.67 |
21.79 |
9 |
71 |
4 |
89 |
89 |
87 |
0.84 |
27.29 |
10 |
72 |
6 |
92 |
91 |
91 |
0.85 |
27.62 |
11 |
75 |
6 |
97 |
94 |
93 |
0.89 |
28.69 |
12 |
76 |
7 |
98 |
96 |
94 |
0.89 |
28.80 |
13 |
77 |
8 |
99 |
97 |
96 |
0.89 |
28.91 |
14 |
78 |
10 |
102 |
100 |
96 |
0.89 |
28.91 |
15 |
80 |
10 |
103 |
101 |
97 |
0.90 |
29.23 |
16 |
81 |
10 |
103 |
103 |
99 |
0.92 |
29.67 |
17 |
81 |
11 |
104 |
104 |
100 |
0.92 |
29.67 |
18 |
82 |
11 |
105 |
105 |
100 |
0.92 |
29.88 |
19 |
83 |
12 |
106 |
106 |
101 |
0.92 |
29.88 |
20 |
84 |
13 |
107 |
107 |
102 |
0.92 |
29.88 |
21 |
85 |
14 |
108 |
108 |
103 |
0.92 |
29.88 |
22 |
86 |
14 |
108 |
108 |
104 |
0.93 |
29.99 |
23 |
87 |
15 |
109 |
109 |
105 |
0.93 |
29.99 |
24 |
89 |
15 |
110 |
110 |
106 |
0.94 |
30.31 |
25 |
90 |
16 |
111 |
111 |
107 |
0.94 |
30.31 |
26 |
91 |
16 |
111 |
112 |
108 |
0.94 |
30.53 |
27 |
92 |
17 |
112 |
113 |
109 |
0.94 |
30.53 |
28 |
92 |
17 |
113 |
114 |
110 |
0.95 |
30.85 |
Note: BIC means abiotic control, IC means Inoculum Control, TI means Test Item.
Analytical results
TOC Analyzer was used to determined the total organic carbon in the samples. The Limit of Detection (LOD) was 0.280 µg C and the limit of quantitation was 1.12 µg C.
The TOC of the test item in solutions in reaction bottles were measured at thebeginning and the end of the test. Prior to the TOC measurement, the solutions wereoscillated for 10 min using ultrasonication to ensure homodisperse.
Table 2. TOC concentrations of the test item in solutions
No. of bottles |
Beginning of the test |
End of the test |
1 (BIC) |
62.46 |
45.64 |
2 (TI-1) |
64.55 |
43.03 |
3 (TI-2) |
65.86 |
42.34 |
4 (TI-3) |
63.75 |
42.54 |
5 (PC) |
/ |
/ |
6 (IC) |
1.36 |
1.20 |
Note: the carbon content of the test item was calculated to be 79.25%.
Description of key information
The test item degraded to 31 % in the modified MITI test. Based on this results, the test item is considerd as not readily biodegradable. As the test item showed more than 20 % biodegradation in the MITI test, the test item can be cosidered as inherently biodegradable.
Key value for chemical safety assessment
- Biodegradation in water:
- inherently biodegradable, fulfilling specific criteria
Additional information
The ready biodegradability of the test item was determined with an activated sludge over a test period of 28 days in the Modified MITI Test (I) according to OECD 301C, Regulation (EC) No. 440/2008 Method C.4-F andGuidelines for the testing of chemicals, SEPA, P.R. China No. 301C "Modified MITI Test (I)" 2004. The test item was tested at a concentration of 100 mg/L in triplicates, corresponding to carbon content of79.25 % and theoretical oxygen uptake of 3.09 mg O2/mg test item.The total organic carbon was measured with TOC Analyzer. The biodegradation of the test item came to 30.85 % after 28 days. The reference item showed 81.74 % degradation after 28 days. In conclusion, the test item is not readily biodegradable as the pass level of 60 % degradation was not reached in a 10-d-window within the 28 days period of the study.
In conclusion, the test item is not readily biodegradable. As more than 20 % biodegradation were observed in the MITI test, the test item can be cosidered as inherently biodegradable.
[Type of water: freshwater]
[Type of water: freshwater]
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