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EC number: 221-329-8 | CAS number: 3068-78-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 30 Jul 1998
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Version / remarks:
- 1984
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.11 (Biodegradation: Activated Sludge Respiration Inhibition Test)
- Version / remarks:
- 1988
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- Hessisches Ministerium für Umwelt, Energie, Jugend, Familie und Gesundheit, Wiesbaden, Germany
- Analytical monitoring:
- no
- Details on sampling:
- - Concentrations: respiration measured in all concentrations and the control
- Sampling method: For the measurement of the respiration rate a well-mixed sample was poured into a Karlsruher flask after exactly 3 h incubation. The oxygen concentration was measured with an oxygen electrode (ECM-multi, Dr. Lange, Germany), and was recorded for 10 min. Samples were continuously stirred on a magnetic stirrer during measurement. The oxygen consumption (in mg O2 L^-1 min^-1) was determined from the most linear part of the respiration curve. - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Test substance was directly weighed into the test flasks and flasks were filled each with 284 mL tap water.
- Differential loading: yes - Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- - Name and location of sewage treatment plant where inoculum was collected: sewage plant Gross-Zimmern, Germany
- Preparation of inoculum for exposure: An aliquot of the sludge suspension was weighed, dried and the ratio of wet sludge (g) to its dry weight (g) was determined. On this basis, aliquots of washed sludge suspension, corresponding to 3.1 g dry material per litre, were made up with tap water. To this mixture, 50 mL synthetic sewage feed per litre was added, starting two days prior to use, and the sludge was kept at room temperature under continuous aeration until use.
- Pretreatment: Sludge was washed twice with tap water by centrifugation. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Test temperature:
- 19 - 20 °C
- pH:
- 7.6 - 8.3
- Dissolved oxygen:
- 5.6 - 8.5 mg/L
- Nominal and measured concentrations:
- 0 (control), 10, 32, 100, 320, 1000 mg/L (nominal)
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 1 L glass flasks and 250 mL Karlsruher flasks
- Fill volume: 500 mL
- Aeration: with compressed air (approx. 0.6 L/min)
- No. of vessels per concentration (replicates): 5
- No. of vessels per control (replicates): 2
- Nutrients provided for bacteria: 16 mL/L synthetic sewage feed (16 g peptone, 11 g meat extract, 3 g urea, 0.7 g NaCl, 0.4 g CaCl2x2H2O, 0.2 g MgSO4x7H2O, 2.8 g K2HPO4; filled up to 1 L with deionized water)
- Activated sludge concentration: 200 mL sludge in 500 mL final volume
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: according to guideline
OTHER TEST CONDITIONS
- Adjustment of pH: no
EFFECT PARAMETERS MEASURED: Oxygen consumption was measured at the end of the 3 h incubation time. - Reference substance (positive control):
- yes
- Remarks:
- 3,5-Dichlorophenol
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Duration:
- 3 h
- Dose descriptor:
- other: EC20
- Effect conc.:
- 203 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Results with reference substance (positive control):
- - Results with reference substance valid: yes
- Relevant effect levels: EC50 (3 h) = 18 mg/L (% inhibition was 14.1, 91.2 and 96.7 at 10, 32, 100 mg/L respectively ) - Validity criteria fulfilled:
- yes
- Remarks:
- For further details please refer to “Any other information on results incl. tables”.
- Conclusions:
- No effects caused by the substance to activated sludge were determined in a 3 h exposure study, resulting in an EC50 > 1000 mg/L.
Reference
BIOLOGICAL RESULTS:
Table 1: Oxygen consumption and inhibition of activated sludge by the test substance.
Concentration [mg/L] | Oxygen consumption [mg 02/L min] | Inhibition % |
control | 0.739 | |
1000 | 0.459 | 37.9 |
320 | 0.764 | -3.4 |
100 | 0.661 | 10.5 |
32 | 0.804 | -8.8 |
10 | 0.696 | 5.9 |
VALIDITY CRITERIA:
- The respiration rates of the two controls did not differ by more than 15 %.
- The 3-hour EC 50 of the reference substance 3,5- Dichlorophenol was 18 mg/L and thus in the range of 5 to 30 mg/L for the used activated sludge batch.
- The concentration of dissolved oxygen did not drop below 2.5 mg/L during the incubation period, and just before the measurements of the respiration rates the oxygen concentrations were at least 6.4 mg/L.
.
Description of key information
EC50 (3 h) > 1000 mg/L (nominal, OECD 209)
Key value for chemical safety assessment
Additional information
The available study (1998) investigates the toxicity of the substance N-[3-(trimethoxysilyl)propylcyclohexylamine] (CAS 3068-78-8) to activated sludge microorganisms and was performed according to OECD 209 and GLP. Activated sludge from a domestic sewage treatment plant was exposed to nominal concentrations of 10, 32, 100, 320 and 1000 mg test item/L for 3 h. Since the substance is expected to hydrolyse rapidly in contact with water, the results from this study refer partly to effects of the hydrolysis products N-[3-(trihydroxysilyl)propylcyclohexylamine] and methanol. After an exposure time of 3 h an inhibition of respiration of 37.9% was recorded in the highest concentration tested, resulting in an EC20 (3 h) = 203 mg/L and EC50 (3 h) > 1000 mg/L (nominal).
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