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Diss Factsheets
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EC number: 221-329-8 | CAS number: 3068-78-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://echa.europa.eu/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_ecotoxicological-information.png)
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 02 - 06 Nov 1998
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Version / remarks:
- 1992
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.1 (Acute Toxicity for Fish)
- Version / remarks:
- 1992
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- Hessisches Ministerium für Umwelt, Energie, Jugend, Familie und Gesundheit, Wiesbaden, Germany
- Analytical monitoring:
- yes
- Remarks:
- ICP - measurement of Si-concentration
- Details on sampling:
- - Concentrations: control and 100 mg/L
- Sampling method: Duplicates from the single test concentration of 100 mg/L and the control were taken from freshly prepared test medium and on Days 2 and 4 of exposure. All samples taken from the 100 mg/L concentration and only one replicate from the control samples were analyzed in the laboratories of Dr. Weßling Laboratorien GmbH, Darmstadt, Germany.
- Sample storage conditions before analysis: Samples were stored in a refrigerator until analysis. - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: 500.4 mg test substance were suspended homogeneously in 5 L test water by intense stirring for 30 min. - Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- TEST ORGANISM
- Common name: zebra fish
- Source: City Zoo, Darmstadt, Germany
- Age at study initiation: juveniles
- Length at study initiation (length definition, mean, range and SD): 3.5 ± 0.1 cm (10 fish from the test fish batch were measured at the start of the test)
- Weight at study initiation (mean and range, SD): 0.64 ± 0.17 g wet weight (10 fish from the test fish batch were measured at the start of the test)
ACCLIMATION
- Acclimation period: 4 weeks
- Acclimation conditions (same as test or not): acclimated to the test temperature in the laboratory, in test water
- Type and amount of food during acclimation: until one day before test start fish were fed with a commercial fish diet (TETRA MIN Hauptfutter)
- Health during acclimation (any mortality observed): During the last 4 weeks prior to the test start, one fish died (mortality rate 2%), all other fish were healthy
FEEDING DURING TEST : no - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 96 h
- Hardness:
- 2.5 mmol/L (= 250 mg/L) as CaCO3
- Test temperature:
- 22 - 23 °C
- pH:
- 7.8 - 8.8
- Dissolved oxygen:
- 7.2 - 8.7 mg/L
- Salinity:
- -
- Conductivity:
- -
- Nominal and measured concentrations:
- Nominal: control, 100 mg/L
Measured (based on Si): 96.2 - 100.3% of nominal - Details on test conditions:
- TEST SYSTEM
- Test vessel: 9 L glass aquaria
- Fill volume: 5 L test medium, 4 L headspace
- Aeration: media slightly aerated during the test
- No. of organisms per vessel: 7
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: according to the guideline
- Alkalinity: 0.8 mmol/L
- Ca/Mg ratio: 4/1
- Intervals of water quality measurement: Temperature, pH and dissolved oxygen concentrations were determined daily. The behaviour of the test substance in the test water was observed once every day.
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 h light/ 8 h dark
- Light intensity: 248 - 255 lx
EFFECT PARAMETERS MEASURED: fish were observed after approx. 2, 24, 48, 72 and 96 h for symptoms of intoxication and mortality
TEST CONCENTRATIONS
- Range finding study : yes. Not according to GLP and data not reported.
- Results used to determine the conditions for the definitive study: yes, tested concentration is based on the results of the range finding test - Reference substance (positive control):
- no
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Details on results:
- - Behavioural abnormalities: no
- Other biological observations: no
- Mortality of control: no
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: no observations, test medium was clear - Sublethal observations / clinical signs:
ANALYTICAL RESULTS:
The measured substance concentrations during the test period were in the range between 96.2 and 100.3 % of the nominal values. Therefore the results are expressed as nominal values. A differentiation between test substance and hydrolysis product was not possible with the used analytical method.
BIOLOGICAL RESULTS:
Further effect values presented in the study report:
- NOEC (96 h) >= 100 mg/L
- LC0 (96 h) >= 100 mg/L
- LC100 (96 h) > 100 mg/L
VALIDITY CRITERIA:
Table 1: Validity criteria for OECD 203.
Criterion from the guideline
Outcome
Validity criterion fulfilled
The mortality in the control(s) should not exceed 10% (or one fish if less than ten are used) at the end of the test
No mortalities
yes
The dissolved oxygen concentration must have been at least 60 per cent of the air
saturation value throughout the test
7.2 - 8.7 mg/L (>60%)
yes
There must be evidence that the concentration of the substance being tested has been
satisfactorily maintained, and preferably it should be at least 80 per cent of the
nominal concentration throughout the test. If the deviation from the nominal
concentration is greater than 20 per cent, results should be based on the measured
concentration.
The measured test item concentrations were between 96 and 100% of the nominal values.
yes
- Validity criteria fulfilled:
- yes
- Conclusions:
- The limit test performed according to the OECD guidance 203 under static conditions, showed no toxic effects to the tested organism Brachydanio rerio up to the highest tested concentration of 100 mg/L (nominal).
Reference
Description of key information
LC50 (96 h) > 100 mg/L (nominal, OECD 203)
Key value for chemical safety assessment
Additional information
In an acute fish toxicity test (1999) according to the OECD guidance 203 and GLP standards, the test organism Brachydanio rerio was exposed to a single nominal concentrations of 100 mg /L. An analytical monitoring took place via ICP by measuring the Si-signal of the test substance in the medium. Samples were taken from the freshly prepared medium and from the aged medium on Day 2 and 4. The recovery of the measured samples was between 96 and 100% of the nominal values. However a differentiation between parent substance and hydrolysis product was not possible with the used analytical method. Since static conditions were used for the test and the hydrolysis of the substance at neutral pH is rapid (half-life = 4.1 min at pH 7), it is assumed that the tested organism was exposed to both the parent compound and the hydrolysis products. The study did not show any toxic effects to fish and resulted in a LC50 (96 h) of > 100 mg/L.
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