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Diss Factsheets
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EC number: 201-781-2 | CAS number: 87-89-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Long-term toxicity to fish
Administrative data
- Endpoint:
- fish, juvenile growth test
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
Data source
Reference
- Reference Type:
- publication
- Title:
- Juvenile Tilapia (Oreochromis niloticus x Oreochromis aureus) Requires Ddietary Myo-inositol for Maximal Growth
- Author:
- Shiau S-Y and Su S-L
- Year:
- 2 005
- Bibliographic source:
- Aquaculture, 243:273– 277
Materials and methods
Test guideline
- Qualifier:
- no guideline available
- Principles of method if other than guideline:
- An 8-week growth experiment was conducted to estimate the dietary requirement of the test substance for juvenile hybrid tilapia.
- GLP compliance:
- not specified
Test material
- Reference substance name:
- Myo-inositol
- EC Number:
- 201-781-2
- EC Name:
- Myo-inositol
- Cas Number:
- 87-89-8
- Molecular formula:
- C6H12O6
- IUPAC Name:
- cyclohexane-1,2,3,4,5,6-hexol
- Test material form:
- solid
- Details on test material:
- - Purity: not reported
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
Test solutions
- Vehicle:
- no
Test organisms
- Test organisms (species):
- other: Oreochromis niloticus x Oreochromis aureus
Study design
- Test type:
- other: Feeding study
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 8 wk
- Post exposure observation period:
- None
Test conditions
- Hardness:
- not reported
- Test temperature:
- 26±1°C
- pH:
- not reported
- Dissolved oxygen:
- not reported
- Conductivity:
- not reported
- Nominal and measured concentrations:
- 150, 250, 350, 450, 600, and 1200 mg/kg diet in the basal diet providing 167, 259, 367, 479, 612, and 1253 mg/kg diet, respectively.
- Reference substance (positive control):
- no
Results and discussion
Effect concentrations
- Key result
- Duration:
- 8 wk
- Dose descriptor:
- LC50
- Effect conc.:
- >= 1 200 other: mg/kg in diet
- Nominal / measured:
- nominal
- Basis for effect:
- mortality
- Remarks on result:
- other: Survival was not affected by dietary treatment.
- Details on results:
- Weight gain was highest for fish fed diets supplemented with ≥367 mg/kg diet, followed by the 259 mg/kg diet, and lowest for fish fed the unsupplemented basal diet. Fish fed ≥367 mg/kg diet had higher food efficiency than fish fed the basal diet. Survival was not affected by dietary treatment. Test substance concentration in liver was highest in fish fed ≥479 mg/kg diet, followed by fish fed 259 and 167 mg/kg diets, and lowest in fish fed the basal diet. Hepatic lipid concentrations were higher in fish fed ≥367 mg/kg diet than fish fed ≤259 mg/kg diet. Growth performance, liver test substance, and lipid concentrations of fish fed the test substance-free diet containing the antibiotic were similar to that of fish fed the test substance-free diet without the antibiotic.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- Survival of fish was not affected by dietary treatment with the test substance at doses up to 1200 mg/kg for 8 weeks. Growth and food consumption were increased at doses of ≥367 mg/kg.
- Executive summary:
An 8-week growth experiment was conducted to estimate the dietary requirement of the test substance for juvenile hybrid tilapia. The test substance was supplemented at 0, 150, 250, 350, 450, 600, and 1200 mg/kg diet in the basal diet providing 0, 167, 259, 367, 479, 612, and 1253 mg/kg diet. Basal diet without test substance but with succinylsulfathiazole to suppress inositol synthesis by intestinal bacteria was included for comparison. Each diet was fed to triplicate groups of tilapia. Fish fed ≥367 mg/kg diet had significantly higher weight gain, followed by fish fed 259 mg/kg diet, and lowest for fish fed the unsupplemented basal diet. Fish fed ≥367 mg/kg diet had higher feed efficiency than fish fed the basal diet. Supplementation of dietary test substance did not affect survival of tilapia. Test substance concentrations in liver were highest in fish fed the ≥479 mg/kg diet, followed by fish fed the 259 and 167 mg/kg diets, and lowest in fish fed the basal diet. Hepatic lipid concentrations were higher in fish fed 367 mg/kg diet than fish fed ≤259 mg/kg diet. Weight gain percentage and test substance concentrations in the liver for the different treatments were analyzed by broken-line regression and indicated that the requirement for dietary inositol in growing tilapia is about 400 mg/kg diet. Addition of an antibiotic to basal diet did not affect the growth and hepatic inositol concentration of tilapia, suggesting that the intestinal microbial synthesis was not a significant source of inositol for tilapia.
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