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Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

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Reference
Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
data from handbook or collection of data
Remarks:
Experimental data of read across substance
Justification for type of information:
Data for the target chemical is summarized based on the structurally similar read across chemicals.
Reason / purpose:
read-across source
Related information:
Composition 1
Reason / purpose:
read-across source
Related information:
Composition 1
Reference:
Composition 0
Qualifier:
according to
Guideline:
other: As mention below
Principles of method if other than guideline:
WoE report is prepared based on short term toxicity to aquatic invertebrates studies
GLP compliance:
not specified
Test material information:
Composition 1
Specific details on test material used for the study:
Name: (2α,4aα,8β)-hexahydro-1,1,5,5-tetramethyl-2H-2,4a-methanonaphthalen-8(5H)-one
Molecular Formula: C15H24O
Molecular Weight: 220.3536 g/mole
InChI:1S/C15H24O/c1-13(2)7-6-11(16)12-14(3,4)10-5-8-15(12,13)9-10/h10,12H,5-9H2,1-4H3/t10-,12+,15-/m0/s1
Smiles:CC1(C)[C@H]2CC[C@]3(C2)[C@@H]1C(=O)CCC3(C)C
Analytical monitoring:
yes
Remarks:
In first study chemical was analytically monitorized
Vehicle:
not specified
Details on test solutions:
1. - Method: Test solutions were prepared by mixing a stock solution of the test substance, which was prepared by adding and stirring the substance under the water surface of the Erlenmeyer flask filled with medium.
Test organisms (species):
other: 1. Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum), 2. Chlorococcales
Details on test organisms:
Details on test organisms
TEST ORGANISM
- Common name: Green algae
Test type:
static
Water media type:
freshwater
Total exposure duration:
72 h
Remarks on exposure duration:
1.0 days exposure given to algae in 2 study
Test temperature:
1. 22.3 - 22.6 ° C
Nominal and measured concentrations:
1. 500, 160, 50, 16, 5.0 mg/L (geometric ratio; square root of 10), control
Details on test conditions:
1. TEST SYSTEM:
- Test vessel: Erlenmeyer flask
- Exposure system: rotary shaking culture (ca. 100 rpm),
- Material, size, headspace, fill volume: test solution volume; 600 mL/control (100 mL/vessel), 300 mL/ concentration (100 mL/vessel), (Separately, a vessel was reserved for analysis at 48 hours.)
- No. of vessels per concentration (replicates): 3 (Separately from that, another vessel for analysis at 48 hours was prepared.)
- No. of vessels per control (replicates):6

EFFECT PARAMETERS MEASURED:
- Measurement of biomass: Cell concentration

OTHER TEST CONDITIONS:
- Light intensity and quality: 98 - 99 microE/m2/s
Reference substance (positive control):
not specified
Key result
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
250 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Remarks on result:
other: 95 % CL: 220 - 270 mg/L
Remarks:
1st study
Duration:
1 d
Dose descriptor:
EC50
Effect conc.:
1 300 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: Physiology and Assimilation efficiency
Remarks on result:
other: 2 study
Validity criteria fulfilled:
not specified
Conclusions:
1. Based on the growth rate inhibition of test organism Pseudokirchneriella subcapitata due to the direct contact with the chemical for 3 days, the effect concentration EC50 was 250 mg/l with the 95 % CL was 220 - 270 mg/L. and the NOEC was observed at the 16 mg/l.
2 .Based on the effect on Physiology and Assimilation efficiency of Chlorococcales (Green Algae Order), by the chemical, the EC50 was determine at 1300 mg/l.
Thus based on the overall studies for (2α,4aα,8β)-hexahydro-1,1,5,5-tetramethyl-2H-2,4a-methanonaphthalen-8(5H)-one (29461 -13 -0), it can be concluded that the test chemical was nontoxic as value ranges from 250 -1300 mg/l and not classified as per the CLP classification criteria.  
Executive summary:

Short term toxicity of (2α,4aα,8β)-hexahydro-1,1,5,5-tetramethyl-2H-2,4a-methanonaphthalen-8(5H)-one (29461 -13 -0) on the growth and other biological activity of aquatic algae was carried out on the basis of it structurally and functionally similar read across chemicals. The studies are as mentioned below:

 

Study 1: Aim of this study was to determine the Growth Inhibition Test of Alga (Pseudokirchneriella subcapitata) by the test substance for the exposure period of 3 days. Test conducted according to OECD Guideline 201 (Freshwater Alga and Cyanobacteria, Growth Inhibition Test). Analytical monitoring was also done using the GC were the Frequency of analysis was at the start of exposure, after 4 hours from start of exposure, and at the end of exposure. Test solutions were prepared by mixing a stock solution of the test substance, which was prepared by adding and stirring the substance under the water surface of the Erlenmeyer flask filled with medium. Test performed on green algae Pseudokirchneriella subcapitata. Test was conducted at the different concentrations 500, 160, 50, 16, 5.0 mg/L (geometric ratio; square root of 10), control. Based on the growth rate inhibition of test organism Pseudokirchneriella subcapitata due to the direct contact with the chemical for 3 days, the effect concentration EC50 was 250 mg/l with the 95 % CL was 220 - 270 mg/L. and the NOEC was observed at the 16 mg/l. Thus on the basis of EC50, chemical was consider as nontoxic and not classified as toxic as per the CLP classification criteria.

Study 2: Toxicity study was carried out for determination of the nature of chemical on the growth of aquatic algae and cyanobacteria. Test conducted under the static system for the total exposure period of 24 hrs. Based on the effect on Physiology and Assimilation efficiency of Chlorococcales (Green Algae Order), by the chemical, the EC50 was determine at 1300 mg/l. Thus on the basis of EC50 value, chemical was concluded as nontoxic and not classified as per the CLP classification criteria. 

Thus based on the overall studies for (2α,4aα,8β)-hexahydro-1,1,5,5-tetramethyl-2H-2,4a-methanonaphthalen-8(5H)-one (29461 -13 -0), it can be concluded that the test chemical was nontoxic as value ranges from 250 -1300 mg/l and not classified as per the CLP classification criteria.  

Description of key information

1. Based on the growth rate inhibition of test organism Pseudokirchneriella subcapitata due to the direct contact with the chemical for 3 days, the effect concentration EC50 was 250 mg/l with the 95 % CL was 220 - 270 mg/L. and the NOEC was observed at the 16 mg/l.

2 .Based on the effect on Physiology and Assimilation efficiency of Chlorococcales (Green Algae Order), by the chemical, the EC50 was determine at 1300 mg/l.

Thus based on the overall studies for (2α,4aα,8β)-hexahydro-1,1,5,5-tetramethyl-2H-2,4a-methanonaphthalen-8(5H)-one (29461 -13 -0), it can be concluded that the test chemical was nontoxic as value ranges from 250 -1300 mg/l and not classified as per the CLP classification criteria.  

Key value for chemical safety assessment

EC50/LC50 for freshwater algae:
250 mg/L

Additional information

Short term toxicity of (2α,4aα,8β)-hexahydro-1,1,5,5-tetramethyl-2H-2,4a-methanonaphthalen-8(5H)-one (29461 -13 -0) on the growth and other biological activity of aquatic algae was carried out on the basis of it structurally and functionally similar read across chemicals. The studies are as mentioned below:

 

Study 1: Aim of this study was to determine the Growth Inhibition Test of Alga (Pseudokirchneriella subcapitata) by the test substance for the exposure period of 3 days. Test conducted according to OECD Guideline 201 (Freshwater Alga and Cyanobacteria, Growth Inhibition Test). Analytical monitoring was also done using the GC were the Frequency of analysis was at the start of exposure, after 4 hours from start of exposure, and at the end of exposure. Test solutions were prepared by mixing a stock solution of the test substance, which was prepared by adding and stirring the substance under the water surface of the Erlenmeyer flask filled with medium. Test performed on green algae Pseudokirchneriella subcapitata. Test was conducted at the different concentrations 500, 160, 50, 16, 5.0 mg/L (geometric ratio; square root of 10), control. Based on the growth rate inhibition of test organism Pseudokirchneriella subcapitata due to the direct contact with the chemical for 3 days, the effect concentration EC50 was 250 mg/l with the 95 % CL was 220 - 270 mg/L. and the NOEC was observed at the 16 mg/l. Thus on the basis of EC50, chemical was consider as nontoxic and not classified as toxic as per the CLP classification criteria.

 

Study 2: Toxicity study was carried out for determination of the nature of chemical on the growth of aquatic algae and cyanobacteria. Test conducted under the static system for the total exposure period of 24 hrs. Based on the effect on Physiology and Assimilation efficiency of Chlorococcales (Green Algae Order), by the chemical, the EC50 was determine at 1300 mg/l. Thus on the basis of EC50 value, chemical was concluded as nontoxic and not classified as per the CLP classification criteria. 

 

 

Thus based on the overall studies for (2α,4aα,8β)-hexahydro-1,1,5,5-tetramethyl-2H-2,4a-methanonaphthalen-8(5H)-one (29461 -13 -0), it can be concluded that the test chemical was nontoxic as value ranges from 250 -1300 mg/l and not classified as per the CLP classification criteria.