Registration Dossier

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Currently viewing:

Administrative data

Link to relevant study record(s)

Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
data from handbook or collection of data
Remarks:
Data is for read across substance
Justification for type of information:
Data for the target chemical is summarized based on the structurally similar read across chemicals.
Reason / purpose:
read-across source
Related information:
Composition 1
Reason / purpose:
read-across source
Related information:
Composition 1
Reference:
Composition 0
Qualifier:
according to
Guideline:
other: As mention below
Principles of method if other than guideline:
WoE report is prepared based on short term toxicity to aquatic invertebrates studies
GLP compliance:
not specified
Test material information:
Composition 1
Specific details on test material used for the study:
Name: (2α,4aα,8β)-hexahydro-1,1,5,5-tetramethyl-2H-2,4a-methanonaphthalen-8(5H)-one
Molecular Formula: C15H24O
Molecular Weight: 220.3536 g/mole
InChI:1S/C15H24O/c1-13(2)7-6-11(16)12-14(3,4)10-5-8-15(12,13)9-10/h10,12H,5-9H2,1-4H3/t10-,12+,15-/m0/s1
Smiles:CC1(C)[C@H]2CC[C@]3(C2)[C@@H]1C(=O)CCC3(C)C
Analytical monitoring:
yes
Vehicle:
not specified
Details on test solutions:
1. - Method: Test solutions were prepared by mixing a stock solution of the test substance, which was prepared by mixing and stirring the test substance and water.
Test organisms (species):
Daphnia magna
Details on test organisms:
1. Details on test organisms
TEST ORGANISM
- Common name: Water flea
- Feeding during test: No

2. - Age at study initiation (mean and range, SD): <=24 h
Test type:
static
Water media type:
freshwater
Total exposure duration:
48 h
Post exposure observation period:
24.0 in 2nd study
Hardness:
2. 16 dH
Test temperature:
1. 20 ° C
2. 20-22°C
pH:
2. 7.6 – 7.7
Nominal and measured concentrations:
1. 350, 230, 160, 104, 69 mg/L (geometric ratio 1.5), control
Details on test conditions:
1. Details on test conditions
TEST SYSTEM
- Type (delete if not applicable): closed (with gas phase)
- Material, size, headspace, fill volume: Test solution volume 400 mL/ concentration (100 mL/vessel)
- Aeration: No
- No. of organisms per vessel: 20 concentration (5 /vessel)
- No. of vessels per concentration (replicates): 4 /concentration
- No. of vessels per control (replicates): 4 /concentration,

WATER PARAMETERS: - Source/preparation of dilution water: dechlorinated tap water

OTHER TEST CONDITIONS: - Photoperiod: room light, 16 hours light hours dark
Reference substance (positive control):
not specified
Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
200 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: 95 % CL: 180 - 230 mg/L
Remarks:
1st study
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
4 730 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: Intoxication
Remarks on result:
other: Effect measured by observing the immobility

1. - Lowest concentration causing 100 % immobility (48 h): > 350 mg/L,

- Highest concentration causing 0 % immobility (48 h): 104 mg/L

Validity criteria fulfilled:
not specified
Conclusions:
1. Based on the immobility of test organism daphnia magna due to the direct contact with the test chemical for 48 hrs, the effect concentration was 200 mg/l with the 95 % CI of 180 - 230 mg/L.
2. Based on the intoxication (Measured by observing the immobility) of daphnia magna by the exposure of chemical, the LC50 was determine to be 4730 mg/l.
Thus based on the overall studies for (2α,4aα,8β)-hexahydro-1,1,5,5-tetramethyl-2H-2,4a-methanonaphthalen-8(5H)-one (29461 -13 -0), it can be concluded that the test chemical was nontoxic as value ranges from 200-4730 mg/l and not classified as per the CLP classification criteria.  
Executive summary:

Short term toxicity of (2α,4aα,8β)-hexahydro-1,1,5,5-tetramethyl-2H-2,4a-methanonaphthalen-8(5H)-one (29461 -13 -0) on the growth and other biological activity of aquatic invertebrates is predicted on the basis of it structurally and functionally similar read across chemicals. The studies are as mentioned below:

 

In the first study toxicity was measured. Aim of this study was to determine the acute Immobilization of test chemical to Daphnia magna for the exposure period of 48 hrs. Test conducted according to OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test). Analytical monitoring was also done using the GC were the Frequency of analysis was at the start of exposure and the end of exposure. Test solutions were prepared by mixing a stock solution of the test substance, which was prepared by mixing and stirring the test substance and water. Test performed at different concentrations 350, 230, 160, 104, 69 mg/L (geometric ratio 1.5), control. Test vessel filled with 400 mL/ concentration (100 mL/vessel) contain 20 daphnia per concentration (5 / vessel). Experiment repeated 4 times with the same condition. Based on the immobility of test organism daphnia magna due to the direct contact with the chemical for 48 hrs, the effect concentration was 200 mg/l with the 95 % CI of 180 - 230 mg/L. Thus on the basis of EC50, chemical was consider as nontoxic and not classified as toxic as per the CLP classification criteria.

Similarly another short term toxicity study was carried out for determination of the nature of chemical on the growth of aquatic invertebrates. Test conducted under the static system for the total exposure period of 24 hrs. <=24 h old daphnia magna were used in the study. Based on the intoxication (Measured by observing the immobility) of daphnia magna by the exposure of chemical, the LC50 was determine to be 4730 mg/l. Based on the LC50 chemical was concluded as nontoxic and not classified as per the CLP classification criteria.

Thus based on the overall studies for (2α,4aα,8β)-hexahydro-1,1,5,5-tetramethyl-2H-2,4a-methanonaphthalen-8(5H)-one (29461 -13 -0), it can be concluded that the test chemical was nontoxic as value ranges from 200-4730 mg/l and not classified as per the CLP classification criteria.  

Description of key information

1. Based on the immobility of test organism daphnia magna due to the direct contact with the test chemical for 48 hrs, the effect concentration was 200 mg/l with the 95 % CI of 180 - 230 mg/L.

2. Based on the intoxication (Measured by observing the immobility) of daphnia magna by the exposure of chemical, the LC50 was determine to be 4730 mg/l.

Thus based on the overall studies for (2α,4aα,8β)-hexahydro-1,1,5,5-tetramethyl-2H-2,4a-methanonaphthalen-8(5H)-one (29461 -13 -0), it can be concluded that the test chemical was nontoxic as value ranges from 200-4730 mg/l and not classified as per the CLP classification criteria.  

Key value for chemical safety assessment

EC50/LC50 for freshwater invertebrates:
200 mg/L

Additional information

Short term toxicity of (2α,4aα,8β)-hexahydro-1,1,5,5-tetramethyl-2H-2,4a-methanonaphthalen-8(5H)-one (29461 -13 -0) on the growth and other biological activity of aquatic invertebrates is predicted on the basis of it structurally and functionally similar read across chemicals. The studies are as mentioned below:

 

In the first study from authoritative database toxicity was measured. Aim of this study was to determine the acute Immobilization of test chemical to Daphnia magna for the exposure period of 48 hrs. Test conducted according to OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test). Analytical monitoring was also done using the GC were the Frequency of analysis was at the start of exposure and the end of exposure. Test solutions were prepared by mixing a stock solution of the test substance, which was prepared by mixing and stirring the test substance and water. Test performed at different concentrations 350, 230, 160, 104, 69 mg/L (geometric ratio 1.5), control. Test vessel filled with 400 mL/ concentration (100 mL/vessel) contain 20 daphnia per concentration (5 / vessel). Experiment repeated 4 times with the same condition. Based on the immobility of test organism daphnia magna due to the direct contact with the chemical for 48 hrs, the effect concentration was 200 mg/l with the 95 % CI of 180 - 230 mg/L. Thus on the basis of EC50, chemical was consider as nontoxic and not classified as toxic as per the CLP classification criteria.

 

Similarly another short term toxicity study from authoritative database 2018, was carried out for determination of the nature of chemical on the growth of aquatic invertebrates. Test conducted under the static system for the total exposure period of 24 hrs. <=24 h old daphnia magna were used in the study. Based on the intoxication (Measured by observing the immobility) of daphnia magna by the exposure of chemical, the LC50 was determine to be 4730 mg/l. Based on the LC50 chemical was concluded as nontoxic and not classified as per the CLP classification criteria.

 

Thus based on the overall studies for (2α,4aα,8β)-hexahydro-1,1,5,5-tetramethyl-2H-2,4a-methanonaphthalen-8(5H)-one (29461 -13 -0), it can be concluded that the test chemical was nontoxic as value ranges from 200-4730 mg/l and not classified as per the CLP classification criteria.