2,2-bis[(allyloxy)methyl]butyl (3-{[({2,2-bis[(allyloxy)methyl]butoxy}carbonyl)amino]methyl}-3,5,5-trimethylcyclohexyl)carbamate

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

25 August 2009 to 14 October 2009

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Version / remarks:

1992

Deviations:

no

Qualifier:

according to guideline

Guideline:

other: State Environmental Protection Administration of China. The Guidelines for the Testing of Chemicals, 203 Fish Acute toxicity test. China Environmental Science Press

Version / remarks:

2004

Deviations:

no

GLP compliance:

yes

Analytical monitoring:

yes

Details on sampling:

The actual concentration of the 100mg/L test solution was analysed at 0 and 96 h.
-Disposal and storage of the sample: Test solutions were centrifuged (12000 rpm) for 5 minutes, the supernatant was collected and then centrifuged (12000 rpm) for 5 minutes again. The final solution was stored at -20°C until analysis.

Vehicle:

yes

Remarks:

methanol

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: 8.882 g of test material was dissolved in 12 mL methanol. The final volume of the solution was measured with a 20 mL graduated flask and was 18 mL. The nominal concentration of the stock solution was 493.4 g/L.
- Chemical name of vehicle: Methanol
- Controls: Potassium dichromate. 5 g of the reference substance which had been dried at 105°C was dissolved in some test water and stirred with a glass rod. The solution was diluted with the test water to 500 mL. The nominal concentration of the stock solution was 10 g/L.

Test organisms (species):

other: Brachydanio rerio

Details on test organisms:

TEST ORGANISM
- Source: Pearl River Fishery Research Institute of Chinese Academic of Fishery Science.
- Age at study initiation: 2 months
- Length at study initiation: 2.735 to 2.985 cm
- Weight at study initiation: 0.120 to 0.236 g
- The test fish were fed to make sure enough eligible fish can be used.

FEEDING DURING TEST: No

ACCLIMATION
- Acclimation period: 7 days

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Hardness:

115.5 mg (expressed as CaCO3)

Test temperature:

23.0 ± 2.0°C

pH:

7.60

Dissolved oxygen:

Not less than 60% of the air saturation value

Nominal and measured concentrations:

Nominal concentration: 100 mg/L

Details on test conditions:

TEST SYSTEM
- Material, size, headspace, fill volume: 0.606 mL of test material stock solution and 3000 mL of test water were put into the container and mixed to start the test.
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- No. of vessels per vehicle control (replicates): 1
- Biomass loading rate: <1.0 g fish/L

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: The chlorine of tap water was removed by aeration,

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 14 hours/day

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Observations: mortalities and responses of test fish were recorded every day during test period. The dead fish were removed as soon as then were found.
- Temperature: the temperature of each test group was measured everyday.
- pH: pH of each test group was measured everyday.
- Dissolved oxygen: the dissolved oxygen of each test group was measured everyday.
- Analysis of test concentrations: the actual concentration of 100mg/L test solution was analysed at 0h and 96h.

Reference substance (positive control):

yes

Remarks:

Potassium dichromate

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

- Analysis of the samples taken from the test solution with nominal concentration (100 mg/L) showed that the measured concentration at 0 and at 96 hours were 1.82 µg/L and 1.42 µg/L, respectively.
- The results showed that dissolved oxygen was 65 to 101%, which was > 60% of the air saturation value; temperature was 21.1 to 21.7°C; pH was 7.06 to 7.90.
- Fish mortalities of blank control and solvent control were both 0%, and fish mortality of test substance group was 0% at end of the test.
- The 96h LC50 of test material (based on the nominal concentration) was > 100 mg/L

Results with reference substance (positive control):

The result of sensitivity test revealed that 24h LC50 of potassium dichromate was 220 mg/L, within the range of 200 to 400 mg/L.

Validity criteria fulfilled:

yes

Conclusions:

Under the conditions of this study the 96h-LC50 of the test material (based on the nominal concentration) was > 100 mg/L

Executive summary:

The acute toxicity of the test material to fish was determined in accordance with the standardised guidelines "State Environmental Protection Administration of China. The Guidelines for the Testing of Chemicals, 203 Fish Acute toxicity test" and OECD 203 and under GLP conditions using Brachydanio rerio. A limit test was performed at 100 mg/L of test material, with no replicates with a blank control and a solvent control (methanol concentration: 100 mg/L). Ten fish were exposed for 96 hours in a static method. Fish mortality from the test material was 0% at end of the test. Fish mortality from the blank and solvent controls were also both 0%. Potassium dichromate was used as reference substance to confirm the validity of the test. Under the conditions of this study the 96h LC50 of the test material (based on the nominal concentration) was > 100 mg/L.

Description of key information

Under the conditions of this study the 96h LC50 of the test material (based on the nominal concentration) was > 100 mg/L.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

100 mg/L

Additional information

The acute toxicity of the test material to fish was determined in accordance with the standardised guidelines "State Environmental Protection Administration of China. The Guidelines for the Testing of Chemicals, 203 Fish Acute toxicity test" and OECD 203 and under GLP conditions using Brachydanio rerio. A limit test was performed at 100 mg/L of test material, with no replicates with a blank control and a solvent control (methanol concentration: 100 mg/L). Ten fish were exposed for 96 hours in a static method. Fish mortality from the test material was 0% at end of the test. Fish mortality from the blank and solvent controls were also both 0%. Potassium dichromate was used as reference substance to confirm the validity of the test. Under the conditions of this study the 96h LC50 of the test material (based on the nominal concentration) was > 100 mg/L.