Disodium [[4-[bis[4-[(sulphonatophenyl)amino]phenyl]methylene]cyclohexa-2,5-dien-1-ylidene]amino]benzenesulphonate

Endpoint summary

Description of key information

Short term toxicity to aquatic invertebrates:

Based on the prediction done using the OECD QSAR toolbox version 3.3 with log kow as the primary descriptor and considering the five closest read across substances, toxicity on daphnia magna was predicted disodium [[4-[bis[4-[(sulphonatophenyl)amino]phenyl]methylene]cyclohexa-2,5-dien-1-ylidene]amino]benzenesulphonate (28983-56-4). Based on the intoxication of test organism the EC50 value was estimated to be 161 mg/l when Disodium [[4-[bis[4[(sulphonatophenyl)amino]phenyl] methylene]cyclohexa-2,5-dien-1-ylidene]amino] benzenesulphonate exposed to daphnia magna for 48 hrs. Based on this value it can be concluded that the substance Disodium [[4-[bis[4-[(sulphonatophenyl) amino]phenyl]methylene] cyclohexa-2,5-dien-1 -ylidene]amino] benzene -sulphonate (28983-56-4) is considered to be nontoxic to aquatic environment and cannot classified as per the criteria mentioned in CLP regulation.        

Toxicity to aquatic algae and cyanobacteria:

Based on the prediction done using the OECD QSAR toolbox version 3.3 with log kow as the primary descriptor and considering the six closest read across substances, toxicity on Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum) was predicted for disodium [[4-[bis[4-[(sulphonatophenyl)amino]phenyl] methylene]cyclohexa-2,5-dien-1-ylidene]amino]benzenesulphonate (28983-56-4). Based on the growth rate inhibition of algae, the EC50 value was estimated to be 208 mg/l when disodium [[4-[bis[4-[(sulphonatophenyl)amino]phenyl] methylene]cyclohexa-2,5-dien-1-ylidene]amino]benzenesulphonate exposed to Pseudokirchneriella subcapitata for 72hrs. Based on this value it can be concluded that the substance Disodium [[4-[bis[4-[(sulphonatophenyl) amino]phenyl]methylene]cyclohexa -2,5-dien-1-ylidene]amino]benzenesulphonate (28983-56-4) is considered to be nontoxic to aquatic environment and cannot classified as per the criteria mentioned in CLP regulation.    

 

Additional information

Summarized result of toxicity of disodium [[4-[bis[4-[(sulphonatophenyl)amino]phenyl]methylene] cyclohexa-2,5-dien-1-ylidene]amino]benzenesulphonate (28983-56-4) on the growth and other activity of aquatic invertebrates, algae and cyanobacteria by considering the data for target as well as RA chemical which was selected on the basis of structural similarity and log Kow as the primary descriptor are as follows:   

Short term toxicity to aquatic invertebrates:

Various predicted data for the target compound disodium [[4-[bis[4-[(sulphonatophenyl)amino]phenyl] methylene]cyclohexa-2,5-dien-1-ylidene]amino]benzenesulphonate (28983-56-4) and experimental studies for supporting weight of evidence studies for its closest read across substance with log Kow as the primary descriptor and on the basis of structural and functional similarity were reviewed for the toxicity on the invertebrates end point which are summarized as below: 

In a prediction done by SSS (2018), using the OECD QSAR toolbox version 3.3 with log kow as the primary descriptor and considering the five closest read across substances, toxicity on daphnia magna was predicted disodium [[4-[bis[4-[(sulphonatophenyl)amino]phenyl]methylene]cyclohexa-2,5-dien-1-ylidene]amino]benzenesulphonate (28983-56-4). Based on the intoxication of test organism the EC50 value was estimated to be 161 mg/l when Disodium [[4-[bis[4[(sulphonatophenyl)amino]phenyl] methylene]cyclohexa-2,5-dien-1-ylidene]amino] benzenesulphonate exposed to daphnia magna for 48 hrs. Based on this value it can be concluded that the substance Disodium [[4-[bis[4-[(sulphonatophenyl) amino]phenyl]methylene] cyclohexa-2,5-dien-1-ylidene]amino] benzenesulphonate (28983-56-4) is considered to be nontoxic to aquatic environment and cannot classified as per the criteria mentioned in CLP regulation.         

 

First predicted study was supported by the structurally and functionally similar read across chemical (3567-69-9) from ABITEC report 2016 was used. Aim of the study was to assess the effect of chemical disodium 4-hydroxy-3-[(4-sulphonatonaphthyl)azo]naphthalenesulphonate on the mobility of daphnia magna. Test was conducted according to OECD Guideline 202. The stock solution 150 mg/l was prepared by dissolving dark red powder in reconstituted water. Test solutions of required concentration as were prepared by mixing the stock solution of the test sample with reconstituted test water. The test substance was tested at the 0, 0, 10, 45, 67.5, 100, 150 mg/l nominal concentration. Potassium dichromate (K2Cr2O7) were used as a reference positive control. Effects on immobilisation were observed for 48 hours by using nonlinear regression by the software Prism 4.0. After the exposure of chemical for 48 hrs 50 % immobility was observed. Based on the immobility of daphnia magna, the median effective concentration (EC50) for the test substance, disodium 4-hydroxy-3-[(4-sulphonatonaphthyl)azo] naphthalenesulphonate, in Daphnia magna was determined to be 203.2 mg/L for immobilisation effects. This value indicates that the substance is likely to be non-hazardous to aquatic invertebrates and cannot be classified as toxic as per the CLP criteria.

 

Similarly in a third supporting weight of evidence study for read across chemical (915-67-3) from ABITEC lab report study 2016 was conducted. Determination of the inhibition of the mobility of daphnids was carried out with the substance 2,7-Naphthalenedisulfonic acid, 3-hydroxy- 4-[(4-sulfo-1-naphthalenyl), sodium salt; Amaranth dye according to OECD Guideline 202. The limit test was performed at 100 mg/l. Effects on immobilisation were observed for 48 hours. The effective concentration (EC8) for the test substance, 2,7-Naphthalenedisulfonic acid, 3-hydroxy-4- [(4-sulfo-1 -naphthalenyl), sodium salt (Amaranth dye), in Daphnia magna was determined to be 100 mg/L on the basis of mobility inhibition effects in a 48 hour study. This value indicates that the substance is likely to be non-hazardous to aquatic invertebrates and cannot be classified as toxic as per the CLP criteria.

 

On the basis of above results for target chemical disodium [[4-[bis[4-[(sulphonatophenyl)amino]phenyl] methylene] cyclohexa-2,5-dien-1-ylidene]amino]benzenesulphonate (28983-56-4) (from OECD QSAR toolbox version 3.3, 2018) and for its read across substance from ABITEC reports it can be concluded that the test substance disodium [[4-[bis[4-[(sulphonatophenyl)amino]phenyl]methylene] cyclohexa-2,5-dien-1-ylidene]amino]benzenesulphonate (28983-56-4) is nontoxic to aquatic environment and cannot be classified as per the criteria mentioned in CLP regulation.       

 

Toxicity to aquatic algae and cyanobacteria:

Various predicted data for the target compound disodium [[4-[bis[4-[(sulphonatophenyl)amino]phenyl] methylene]cyclohexa-2,5-dien-1-ylidene]amino]benzenesulphonate (28983-56-4) and experimental studies for supporting weight of evidence studies for its closest read across substance with log Kow as the primary descriptor and on the basis of structural and functional similarity were reviewed for the toxicity on the aquatic algae and cyanobacteria end point which are summarized as below: 

In a prediction done by SSS (2018), using the OECD QSAR toolbox version 3.3 with log kow as the primary descriptor and considering the six closest read across substances, toxicity on Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum) was predicted for disodium [[4-[bis[4-[(sulphonatophenyl)amino]phenyl] methylene]cyclohexa-2,5-dien-1-ylidene]amino]benzenesulphonate (28983-56-4). Based on the growth rate inhibition of algae, the EC50 value was estimated to be 208 mg/l when disodium [[4-[bis[4-[(sulphonatophenyl)amino]phenyl] methylene]cyclohexa-2,5-dien-1-ylidene]amino]benzenesulphonate exposed to Pseudokirchneriella subcapitata for 72hrs. Based on this value it can be concluded that the substance Disodium [[4-[bis[4-[(sulphonatophenyl) amino]phenyl]methylene]cyclohexa -2,5-dien-1-ylidene]amino]benzenesulphonate (28983-56-4) is considered to be nontoxic to aquatic environment and cannot classified as per the criteria mentioned in CLP regulation.      

First predicted study was supported by the structurally and functionally similar read across chemical (3567-69-9) from ABITEC report 2016 was used. Freshwater algal growth inhibition test was carried out on Desmodesmus subspicatus with the substance Disodium 4-hydroxy- 3-[(4-sulphonatonaphthyl) azo]naphthalenesulphonate according to OECD Guideline 201. The test substance was dissolved in OECD growth medium and tested at the concentrations 0, 2.6, 5.6, 12.0, 27.0, 59.0 and 130 mg/L. Effects on the growth rate of the organism were studied. The median effective concentration (ErC50) for the test substance, Disodium 4-hydroxy-3-[(4-sulphonatonaphthyl)azo] naphthalenesulphonate, in Desmodesmus subspicatus was determined to be 265.1 mg/L. Based on this ErC50 value and after comparing with CLP criteria for aquatic classification of the substance it is concluded that the substance Disodium 4-hydroxy-3-[(4-sulphonatonaphthyl) azo] naphthalenesulphonate does not exhibit toxicity to aquatic algae (Desmodesmus subspicatus).

      

Similarly in a third supporting weight of evidence study for read across chemical (915-67-3) from ABITEC lab report study 2016 was conducted. Freshwater algal growth inhibition test was carried out on Desmodesmus subspicatus with the substance 2,7-Naphthalenedi- sulfonic acid, 3-hydroxy-4-[(4-sulfo- 1-naphthal - enyl), sodium salt (Amaranth dye) according to OECD Guideline 201. The stock solution (200 g/L) was prepared by dissolving brown powder in OECD growth medium. Test solutions of required concentration were prepared by mixing the stock solution of the test sample with OECD growth medium and inoculum culture. The test substance was dissolved in OECD growth medium and tested at the concentrations 0, 12.5, 25, 50, 100, 200 mg/L. Effects on the growth rate of the organism were studied. The median effective concentration (ErC50) for the test substance, 2,7-Naphthalenedisulfonic acid, 3-hydroxy-4- [(4-sulfo-1- -naphthalenyl), sodium salt, in Desmodesmus subspicatus was determined to be 356.2 mg/L. This value indicates that the substance is likely to be non-hazardous to aquatic algae and cannot be classified as toxic as per the CLP criteria.

 

On the basis of above results for target chemical disodium [[4-[bis[4-[(sulphonatophenyl)amino]phenyl] methylene] cyclohexa-2,5-dien-1-ylidene]amino]benzenesulphonate (28983-56-4) (from OECD QSAR toolbox version 3.3, 2018) and for its read across substance from ABITEC reports it can be concluded that the test substance disodium [[4-[bis[4-[(sulphonatophenyl) amino]phenyl]methylene] cyclohexa-2,5-dien-1-ylidene]amino]benzenesulphonate (28983-56-4) is nontoxic to aquatic environment (algae and cyanobacteria) and cannot be classified as per the criteria mentioned in CLP regulation.       

 

Hence on the basis of above studies for the toxicity of disodium [[4-[bis[4-[(sulphonatophenyl)amino] phenyl]methylene] cyclohexa-2,5-dien-1-ylidene]amino]benzenesulphonate on aquatic invertebrates and algae, it can be concluded that the chemical was nontoxic to aquatic environment and cannot be classified as per the criteria mentioned in CLP regulation.