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EC number: 207-395-0 | CAS number: 467-62-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Endpoint summary
Administrative data
Key value for chemical safety assessment
Genetic toxicity in vitro
Link to relevant study records
- Endpoint:
- in vitro gene mutation study in bacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
- Justification for type of information:
- Data is from publication.
- Qualifier:
- according to guideline
- Guideline:
- other: As mention below
- Principles of method if other than guideline:
- To evaluate the mutagenic potential of Pararosaniline in Salmonella typhimurium strains by Reverse bacterial mutation assay
- GLP compliance:
- not specified
- Type of assay:
- bacterial reverse mutation assay
- Specific details on test material used for the study:
- - Name of test material : 4,4',4''-Triaminotrityl alcohol
- Molecular formula : C19H19N3O
- Molecular weight : 305.379 g/mol
- Smiles notation : C(c1ccc(N)cc1)(c1ccc(N)cc1)(c1ccc(N)cc1)O
- InChl : 1S/C19H19N3O/c20-16-7-1-13(2-8-16)19(23,14-3-9-17(21)10-4-14)15-5-11-18(22)12-6-15/h1-12,23H,20-22H2
- Substance type: Organic
- Physical state: Solid - Target gene:
- Histidine
- Species / strain / cell type:
- S. typhimurium, other: TA1535, TA1536, TA1537, TA1538, TA98, and TAI00
- Details on mammalian cell type (if applicable):
- Not applicable
- Additional strain / cell type characteristics:
- other: All strains are uvrB-deficient (to reduce the repair of chemically induced lesions in the DNA) and are rfa In addition strainsTA98 and TAI00 contain the plasmid pKMIOl, which makes them more sensitive than their parent strains.
- Cytokinesis block (if used):
- not specified
- Metabolic activation:
- with and without
- Metabolic activation system:
- S9 -a metabolic activation system prepared from the livers of randomly bred Sprague-Dawley rats that had been pretreated with Aroclor1254.
- Test concentrations with justification for top dose:
- 0 and 250 ug/plate
- Vehicle / solvent:
- Yes, but not specified
- Untreated negative controls:
- not specified
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- not specified
- Positive controls:
- yes
- Positive control substance:
- not specified
- Details on test system and experimental conditions:
- Details on test system and conditions
METHOD OF APPLICATION: in agar (plate incorporation)
DURATION
- Exposure duration: 12 hour
Other; The strains were checked periodically for relevant genetic markers and for rfa by crystal violet sensitivity; T A98 and T AI00 were checked for ampicillin resistance. - Rationale for test conditions:
- Not specified .
- Evaluation criteria:
- The numbers of Histidine-independent revertants for each Salmonella typhimurium strain were taken from the linear portion of dose-response curves after the background was subtracted. The range of spontaneous revertants was 25-55 for TA1535, 7-25 for TA1537, 10-30 for TA1538, 30-60 for TA98, and 100-180 for TA100.A positive response was defined as a reproducible, dose-related increase in the number of revertants. Usually, a chemical was mutagenic in more than one strain.
- Statistics:
- Mean ±standard deviation was observed.
- Key result
- Species / strain:
- S. typhimurium, other: TA1535, TA1536, TA1537, TA1538, TA98 and TAI00
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not specified
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not specified
- Positive controls validity:
- valid
- Remarks on result:
- other: No mutagenic effect were observed.
- Conclusions:
- Pararosaniline (467-62-9) was evaluated for its mutagenic potential in Salmonella typhimurium strains TA1535, TA1536, TA1537, TA1538, and TA98and TAI00 by bacterial reverse mutation assay. The test results were considered to be negative in the presence and absence of S9 mix.
- Executive summary:
Pararosaniline was assessed for its possible mutagenic potential. For this purpose bacterial reverse mutation assay was performed on Salmonella typhimurium strains TA1535, TA1536, TA1537, TA1538, TA98, and TAI00.The test material was exposed at the concentration of 0 and 250ug /plate byplate incorporation Method.The test material was exposed in the presence and absence of metabolic activation.The numbers of Histidine-independent revertants for each Salmonella typhimurium strain were taken from the linear portion of dose-response curves after the background was subtracted. The range ofspontaneousrevertantswere as per the control. No mutagenic effect was observed. Therefore Pararosaniline was considered to be non mutagenic in Salmonella typhimurium strains TA1535, TA1536, TA1537, TA1538, TA98 and TAI00. Hence the substance cannot be classified as gene mutant in vitro.
Reference
Endpoint conclusion
- Endpoint conclusion:
- no adverse effect observed (negative)
Genetic toxicity in vivo
Endpoint conclusion
- Endpoint conclusion:
- no study available
Additional information
Genotoxicity In-vitro
Various publications were reviewed to determine the mutagenic nature of 4,4',4''-Triaminotrityl alcohol ;Other name; Pararosaniline (467-62-9). The studies are as mentioned below:
Experimental study on genetic toxicity in vitro was conducted by Vincent F. Simmon (Journal of the National Cancer Institute, 1979) to determine the mutagenic nature of target substance Pararosaniline (467-62-9) by AMES test . Pararosaniline was assessed for its possible mutagenic potential. For this purpose bacterial reverse mutation assay was performed on Salmonella typhimurium strains TA1535, TA1536, TA1537, TA1538, TA98, and TAI00.The test material was exposed at the concentration of 0 and 250ug /plate byplate incorporation Method.The test material was exposed in the presence and absence of metabolic activation.The numbers of Histidine-independent revertants for each Salmonella typhimurium strain were taken from the linear portion of dose-response curves after the background was subtracted. The range of spontaneous revertants were as per the control. No mutagenic effect was observed. Therefore Pararosaniline was considered to be non mutagenic in Salmonella typhimurium strains TA1535,TA1536, TA1537, TA1538, TA98 and TAI00. Hence the substance cannot be classified as gene mutant in vitro.
Supporting experimental study on genetic toxicity in vitro was conducted by Vincent F. Simmon (Journal of the National Cancer Institute, 1979) to determine the mutagenic nature of target substance Pararosaniline (467-62-9) by Liquid incubation assay. Pararosaniline was assessed for its possible mutagenic potential. For this Liquid incubation assay was performed on Salmonella typhimurium strains TA1535 and TAI00.The test material was exposed at the concentration of 0 and0.78µmolebyplate incorporation Method.The test material was exposed in the presence and absence of metabolic activation. The numbers of Histidine-independent revertants for each Salmonella typhimurium strain were taken from the linear portion of dose-response curves after the background was subtracted. The range ofspontaneousrevertantswere as per the control. No mutagenic effect was observed. Therefore Pararosaniline was considered to be non mutagenic in Salmonella typhimurium strains TA1535 and TAI00. Hence the substance cannot be classified as gene mutant in vitro.
Another supporting experimental study on genetic toxicity in vitro was conducted by J. Mirsalis et al.( Abstracts of the fifteenth annual meeting of the environmental mutagen society held at San Antonio, Texas March 3–6, 1983 ,Environmental and Molecular Mutagenesis,19 83) to determine the mutagenic nature of target substance Pararosaniline (467-62-9). Genetic toxicity in vitro for Para- Rosaniline was assessed for its possible mutagenic potential. For this purpose Unscheduled DNA assay was performed. Hepatocytes from male Fischer-344 rats were incubated with3H-TdR along with test substance at the test concentration of2.2ug/ml. Positive and negative controls were used .Unscheduled DNA synthesis was measured by quantitative autoradiography as net grains/nucleus (NG). No mutagenic effects were observed in test substance as compared to the value of positive and negative control. Therefore Para- Rosaniline was considered to be non mutagenic for Unscheduled DNA assay. Hence the substance cannot be classified as gene mutant in vitro
In another Another supporting experimental study on genetic toxicity in vitro was conducted by S.R. Haworth et al.( Abstracts of the twelfth annual meeting of the environmental mutagen society, Environmental Mutagenesis,19 81) to determine the mutagenic nature of target substance Pararosaniline (467-62-9). Pararosaniline was assessed for its possible mutagenic potential. For this purpose bacterial reverse mutation assay was performed on Salmonella typhimurium strains TA98 and TAI00.TheB6C3F1mice were fed with the test material. The test substance was exposed to the plates (Salmonella strain TA 98 and TA 100) in the form of urine collected from male and female mouse. The test material was exposed at the concentration of 0.75 ml, 0.5 ml, 0.2 ml and 0.05 ml of neat urinebyplate incorporation Method.The test results were considered to be positive in the presence and absence. Urine from p-rosaniline treated mice exhibited mutagenic activity on TA98 and Tal00 in the male mouse and on TA98 alone in the female mouse. The mutagenic activity was increased in the presence of S9. Therefore Pararosaniline was not considered to be mutagenic in Salmonella typhimurium strains TA98 and TAI00 in the presence and absence of S9. But as the detail study is not available, the test results were considered to be ambiguous. Hence test substance cannot be classified as mutagenic.
Based on the data available for the target chemical from various detailed experimental study , it is concluded that 4,4',4''-Triaminotrityl alcohol ;Other name; Pararosaniline (467-62-9)does not exhibit gene mutation in vitro. Hence the test chemical is not likely to classify as a gene mutant in vitro.
Justification for classification or non-classification
Thus based on the above annatation and CLP criteria for the target chemical from various detailed experimental study , it is concluded that 4,4',4''-Triaminotrityl alcohol ;Other name; Pararosaniline (467-62-9)does not exhibit gene mutation in vitro. Hence the test chemical is not likely to classify as a gene mutant in vitro.
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