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EC number: 201-029-3 | CAS number: 77-47-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Study period:
- 1983
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study with acceptable restrictions
Data source
Reference
- Reference Type:
- publication
- Title:
- Salmonella mutagenicity test Results for 250 Chemicals
- Author:
- Steve Haworth, Timothy Lawlor, Kristien Mortelmans, William Speck and Errol Zeiger
- Year:
- 1 983
- Bibliographic source:
- Environ Mutagen. 1983;5 Suppl 1:1-142
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Deviations:
- yes
- Remarks:
- only tested on 4 out of 5 recommended bacteria strains
- GLP compliance:
- no
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Hexachlorocyclopentadiene
- EC Number:
- 201-029-3
- EC Name:
- Hexachlorocyclopentadiene
- Cas Number:
- 77-47-4
- Molecular formula:
- C5Cl6
- IUPAC Name:
- hexachlorocyclopentadiene
- Test material form:
- liquid
Constituent 1
Method
- Target gene:
- his
Species / strain
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Details on mammalian cell type (if applicable):
- CELLS USED
- Source of cells: Salmonella typhimurium strains obtained from Dr. Bruce Ames, University of California, Berkeley.
- Metabolic activation:
- with and without
- Metabolic activation system:
- Liver S-9 mix from Aroclor 1254-induced male Sprague-Dawley rats and male Syrian hamsters
- Test concentrations with justification for top dose:
- 0.00, 0.03, 0.10, 0.30, 1.00, 3.30, 10.00, 33.30, 100.00 ug/plate
The highest test dose was 10 mg/plate if no toxicity was apparent in the preliminary toxicity determination, or the upper limit of solubility was used. If toxicity was observed, the doses were chosen so that the high dose exhibited some degree of toxicity. - Vehicle / solvent:
- - Vehicle(s)/solvent(s) used: distilled water; dimethyl sulfoxide (DMSO); ethanol; acetone
- Justification for choice of solvent/vehicle: The first choice of solvent was distilled water. Dimethyl sulfoxide (DMSO) was used if the chemical was insoluble or not sufficiently soluble in water, and ethanol (95%) or acetone was used if the chemical was not soluble or stable in DMSO.
Controls
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- Positive controls:
- yes
- Positive control substance:
- 9-aminoacridine
- sodium azide
- other: 2-aminoanthracene (Tested on all strains in presence of S-9); 4-nitro-o-phenylenediamine (Tes ted on TA98 without S-9)
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: preincubation
- Cell density at seeding (if applicable): 0.05ml cells added to each culture tube
DURATION
- Preincubation period: The test mixture was vortexed and allowed to incubate for 20 min at 37 °C.
- Expression time (cells in growth medium): The plates were inverted and incubated at 37°C for 48h.
NUMBER OF REPLICATIONS: At least 5 doses of each test chemical were tested on each strain in the presence of S-9 mix or buffer. Three plates were used and the experiment was repeated no less than 1 week after completion of the initial test. - Rationale for test conditions:
- The preincubation procedure was selected because of evidences that it was no less sensitive than the plate test while being more effective for various chemicals.
- Evaluation criteria:
- A 'positive' response was indicated by a reproducible, dose-related increase in histidine-independent (revertant) colonies in any one strain/activation combination. A negative response is obtained when no increase in revertant colonies is observed. An 'equivocal' or 'questionable' response was applied to low-level responses that were not reproducible or dose-related, or to results that showed a definite trend but with which the investigator did not feel comfortable making a positive or negative decision.
- Statistics:
- The data were evaluated using an analysis based on the models presented by Margolin et al (1981).
Results and discussion
Test resultsopen allclose all
- Species / strain:
- S. typhimurium TA 98
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not specified
- Vehicle controls validity:
- valid
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not specified
- Vehicle controls validity:
- valid
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 1535
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not specified
- Vehicle controls validity:
- valid
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 1537
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not specified
- Vehicle controls validity:
- valid
- Positive controls validity:
- valid
Any other information on results incl. tables
See attached background material for full results table.
Applicant's summary and conclusion
- Conclusions:
- Hexachlorocyclopentadiene was not considered to be mutagenic mutagenic to Salmonella typhinurium strains TA98, TA100, TA1535 or TA 1537 in this bacterial reverse mutation assay, both with and without metabolic activation.
- Executive summary:
The in vitro genotoxicity in bacteria of the test substance was determined using a preincubation procedure, which was a modification of the Salmonella/mammalian microsome test of Ames et al [1975] and similar to the OECD Testing Guideline 471 (non GLP) with deviations.
At least five doses of test chemical, in addition to the concurrent solvent and positive controls, were tested on each bacteria strain in the presence of S-9 mix or buffer. A positive response was indicated by a reproducible, dose-related increase. Controls were valid.
Hexachlorocyclopentadiene was considered to be non-mutagenic under the conditions of the test, for both methods, with and without metabolic activation.
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