2,2'-phenyliminodiethanol

Toxicity to microorganisms

Link to relevant study record(s)

Reference

Endpoint:

activated sludge respiration inhibition testing

Type of information:

experimental study

Adequacy of study:

key study

Study period:

26 to 27 Jan 1995

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

test procedure in accordance with national standard methods with acceptable restrictions

Remarks:

only basic data given; legally binding classification (KBwS, WGK 2)

Qualifier:

according to guideline

Guideline:

DIN 38412-8 (Pseudomonas Zellvermehrungshemmtest)

GLP compliance:

not specified

Specific details on test material used for the study:

SOURCE OF TEST MATERIAL- Source and lot/batch No.of test material: Merck (Name in KBWS database: N,N-Di(2hydroxyethyl)anilin)- Expiration date of the lot/batch: no data- Purity test date: no data

Analytical monitoring:

yes

Details on sampling:

not specified

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)- Method: stock solution (10 g/L) prepared by stirring for 24 h and subsequent pressure filtration (0.2 µm Nylon filter)

Test organisms (species):

Photobacterium phosphoreum

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

17 h

pH:

stock solution (10 g/L): 7.15

Nominal and measured concentrations:

nominal: 0 (control), 15.63, 31.25, 62.5, 125, 250, 500, 1000, 2000, 4000, and 8000 mg/L (verified by TOC analysis)

Key result

Duration:

17 h

Dose descriptor:

EC50

Effect conc.:

> 4 000 - < 8 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth inhibition

Key result

Duration:

17 h

Dose descriptor:

EC10

Effect conc.:

> 2 000 - < 4 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth inhibition

Growth inhibition

Test item conc. (mg/L)	Average TE/F at 463 nm	Inhibition of cell multiplication (%)
control	900	-
15.63	570	-97.37
62.5	550	-25.00
31.25	570	-25.00
125	540	-20.61
250	540	-18.42
500	530	-16.23
1000	530	-16.23
2000	450	1.32
4000	330	27.63
8000	17	96.27

TE/F: turbidity measurement

Description of key information

The inhibition of the degradation activity of activated sludge is not anticipated when introduced in appropriately low concentrations.

Additional information

The assessment of the toxicity to microorganisms is based on effect data taken from the “Commission for the Evaluation of Substances Hazardous to Waters” (KBWS) data sheet no. 1385, which is part of the German Federal Water Act (Article 19g). Based on the collected data a substance is classified according to its water-hazardous properties (WGK 1 to 3). 2,2 -phenyliminodiethanol was classified as WGK 2, which indicates a hazard to waters. As the list with the classified substances was published in the Bundesanzeiger (Federal Ministry of Justice, 2005. Bundesanzeiger No. 142a), the classification is legally binding for the industry.

The effect of 2,2'-phenyliminodiethanol (CAS 120 -07 -0) on the growth inhibition of Pseudomonas putida was studied according to German Industrial Standard DIN 38412 -8 (KBWS, 1995). The tested concentration range ranged between 15 and 8000 mg/l. The 17-h EC50 was determined to be in the range between 4000 and 8000 mg/L. The 17 -h EC10 was assessed to be between 2000 and 4000 mg/L. Based on the available data it can be concluded that the inhibition of the degradation activity of activated sludge is not anticipated when the substance is introduce in appropriately low concentrations.