2,2'-phenyliminodiethanol

Toxicity to aquatic algae and cyanobacteria

Link to relevant study record(s)

Reference

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

supporting study

Study period:

Test start: 05 Dec 1994

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

test procedure in accordance with national standard methods with acceptable restrictions

Remarks:

only basic data given; legally binding classification (KBwS, WGK 2)

Qualifier:

according to guideline

Guideline:

other: Water quality; fresh water algal growth inhibition test with Scenedesmus subspicatus and Selenastrum capricornutum

Version / remarks:

ISO 8692:1989; German version EN 28692:1993

GLP compliance:

not specified

Specific details on test material used for the study:

SOURCE OF TEST MATERIAL- Source and lot/batch No.of test material: Merck (Name in KBWS database: N,N-Di(2hydroxyethyl)anilin)- Expiration date of the lot/batch: no data- Purity test date: no data

Analytical monitoring:

no

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)- Method: addition of 625 mg/L test item to 500 mL test medium, stirred for 0.5 h

Test organisms (species):

Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)

Details on test organisms:

TEST ORGANISM - Strain: Scenedesmus subspicatus CHODAT - Source (laboratory, culture collection): laboratory culture - Age of inoculum (at test initiation): - Method of cultivation: cultured in 100-mL Erlenmeyer flasks in temperature-controlled room with uniform lighting (fluorescent tubes), pre-culture started with 7-d old algae from stock culture, pre-culture medium according to guideline

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test temperature:

23± °C

pH:

stock solution (1250 mg/L): 7.26

Nominal and measured concentrations:

nominal: tested range: 156.25 to 1000 mg/L

Details on test conditions:

TEST SYSTEM - Test vessel: algae test apparatus with quasi-continuous determination of optical density (Abimed) - Initial cells density: 0.02 exteinction units = 10000 cells/mL - Control end cells density: not reported - No. of vessels per concentration (replicates): not reported - No. of vessels per control (replicates): not reported GROWTH MEDIUM - Standard medium used: yes - Detailed composition if non-standard medium was used: TEST MEDIUM / WATER PARAMETERS - Source/preparation of dilution water: test medium according to guideline OTHER TEST CONDITIONS - Sterile test conditions: yes - Adjustment of pH: no - Photoperiod: continuous - Light intensity and quality: 17 W/m² (fluorescent lamps) EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : - Determination of cell concentrations: fluorimeter (400-500 nm; emission: 685 nm) TEST CONCENTRATIONS - Spacing factor for test concentrations: - Justification for using less concentrations than requested by guideline: - Range finding study - Test concentrations: - Results used to determine the conditions for the definitive study:

Reference substance (positive control):

no

Key result

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

418 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

393 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

biomass

Key result

Duration:

72 h

Dose descriptor:

EC10

Effect conc.:

172 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

biomass

Duration:

72 h

Dose descriptor:

NOEC

Effect conc.:

156 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

biomass

Validity criteria fulfilled:

yes

Description of key information

With high probability acutely not harmful to algae.

Key value for chemical safety assessment

EC50 for freshwater algae:

313 mg/L

EC10 or NOEC for freshwater algae:

246 mg/L

Additional information

For the assessment of toxicity of the substance to algae experimental and calculated data available. The trend analysis was performed with QSAR Toolbox v4.4. The 72 -h ErC10 was determined to be 246 mg/L and the 72 -h EC50 was estimated to be 313 mg/L (based on growth rate BASF SE, 2021).

In addition experimental data are available from the “Commission for the Evaluation of Substances Hazardous to Waters” (KBWS) data sheet no. 1385, which is part of the German Federal Water Act (Article 19g). Based on the collected data a substance is classified according to its water-hazardous properties (WGK 1 to 3). 2,2 -phenyliminodiethanol was classified as WGK 2, which indicates a hazard to waters. As the list with the classified substances was published in the Bundesanzeiger (Federal Ministry of Justice, 2005. Bundesanzeiger No. 142a),

the classification is legally binding for the industry. 

The KBWS contains an abstract on the algae test according to DIN EN 28692 (= ISO 8692). The test species wasDesmodesmus subspicatus. Test item concentrations were not analytically verified, but can be assumed to be stable based on the analytical results from an acute toxicity study to daphnids (BASF AG, 1998). The 72 -h ErC50 was determined to be 418 mg/L based on growth rate (72 -h EbC50 = 393 mg/L). The EC10 was only determined for biomass (72 -h EbC10 = 172 mg/L; KBWS, 1995). As the endpoint biomass is generally somewhat lower than the growth rate and can therefore be considered as a conservative value. Since there is only an abstract of the algae test available, the information on the test metods and substance details is limited. Therefore, the QSAR Toolbox calculations were marked as key studies and their endpoints were used as key values for the toxcity assessment and farther aquatic PNECs calculation.

Based on the available calculated and experimental data, 2,2 -phenyliminodiethanol is assessed to be not acutely harmful to algae with high probability. Chronic effects are not expected as well.