Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 418-200-5 | CAS number: 69227-51-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- January 1993
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: conducted under GLP using OECD guidelines
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 301 E (Ready biodegradability: Modified OECD Screening Test)
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- - Source and lot/batch No.of test material: 0691
- Expiration date of the lot/batch: not given
- Purity test date: 49.9% in water solution
STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: 5 oC in the dark
OTHER SPECIFICS:
colorless liquid
pH = 6.84 - Oxygen conditions:
- aerobic
- Inoculum or test system:
- natural soil
- Remarks:
- soil from Sibersdorf
- Details on inoculum:
- Inuculum from soil (soil from Siebersdorf, 1988). The suitability of the soil was checked with two reference substances (sodium acetate/ sodium benzoate). 100 gr soil were suspended in 1000 ml drinking water. After stirring for 60 minutes the suspension was allowded to settle for stirring for 30 minutes. the supernatant was filtered, and the first 200 ml being discarded. The filtrate then was aerated immediately with filtered compressed air for 15 minutes.
- Duration of test (contact time):
- ca. 42 d
- Initial conc.:
- 21.7 mg/L
- Based on:
- DOC
- Parameter followed for biodegradation estimation:
- DOC removal
- Details on study design:
- Principle
A predetermined amount of the test substance is dissolved in a mainly anorganic medium. The solution is inoculated with a small number of polyvalent microorganisms and aerated at 20-25 °C in the dark or at least in diffuse light only. The degradation is checked bu DOC analysis.
Procedure
One reaction vessel per group was each filled with 900 ml of the nutrient solution, containing the test substance in groups A and B respectively the reference substance in group PK and inoculated with 0.5 ml of the inoculum. The opening of the vessels was covered with aluminum foil in such a way that the exchange of air was guaranteed. Then the vessels were inserted in a shaking machune and kept there until the end of the study. Vessels were shielded from light. The room temperature was 21 °C, humidity in the range of 55-60%.
Drawing of samples
At the schedualed terms 30 ml were taken from each reaction vessel and centrifuged (4000 g, 15 min, at room temperature). The supernatants were preserved with 0.05 ml HgCl2 (1%) per 10 ml sample and were stored at 4 °C until DOC measurement. - Reference substance:
- benzoic acid, sodium salt
- Remarks:
- 34.28 mg sodium benzoate (i.e 20 mg DOC were dissolved in 1000 ml nutrient solution immediately before use.
- Key result
- Parameter:
- % degradation (DOC removal)
- Value:
- ca. 9
- Sampling time:
- 42 d
- Remarks on result:
- other: see remarks below
- Details on results:
- The DOC removal markedly less than 5% was calculated for test substance group a over the whole examination period of 42 days. In test substance group B almost the same results were gained, except for days 28 and 42 where DOC removal of 13% and 15% were calculated, degradation was also below 5% at all terms. Calculating DOC removal for both substance groups combined, biodegradation did not exceed 9%.
The degradation of the reference substance was 93% on day 7. A DOC removal of 100% was reached on day 21. - Validity criteria fulfilled:
- yes
- Interpretation of results:
- under test conditions no biodegradation observed
- Conclusions:
- The substance is not biodegradable under test conditions.
- Executive summary:
The test substance at a concentration of about 20 mg DOC per liter was incubated in an aerobic aqueous medium according to OECD guideline 301E.
The DOC removal markedly less than 5% was calculated for test substance group a over the whole examination period of 42 days. In test substance group B almost the same results were gained, except for days 28 and 42 where DOC removal of 13% and 15% were calculated, degradation was also below 5% at all terms. Calculating DOC removal for both substance groups combined, biodegradation did not exceed 9%.
The substance is not biodegradable under test conditions.
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- March-July 2013
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Study conductd under GLP under OECD and Chinese guidelines
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
- Version / remarks:
- and Chinese guidelines
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
N/A - Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge (adaptation not specified)
- Details on inoculum:
- Activated sludge
Source: LiedeSewage Treatment Plant og Guangzhou
Treatment: the sludge was washed by centrifugation, the supernatant liquid phase was decanted and the solid material resuspended in a mineral medium. A homogenized aliquot of the final sludge suspension was weighted. Based on the ration, calculated amount of wet sludge was suspended in mineral medium to obtain a concentration equivalent to 2.4 g dry material per liter. Appropriate volume of inoculum was inculated in each bottle to give a final concentration of 28 mg dry material per liter. - Duration of test (contact time):
- ca. 28 d
- Initial conc.:
- 27 mg/L
- Based on:
- test mat.
- Initial conc.:
- 32 mg/L
- Based on:
- test mat.
- Remarks:
- toxicity control
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- Inoculum: Activated sludge
The sludge was washed by centrifugation, the supernatant liquid phase was decanted and the solid material resuspended in a mineral medium. A homogenized aliquot of the inal sludge suspenstion was weighted. Based on this ration, calculated amount of wet sludge was suspended in a mineral medium to obtain a concentration equivalent to 2.4 g dry material per liter. Appropriate volume of inoculum was inoculated in each bottle to give a final concentration of 28 mg dry material per liter.
20 L of test medium was prepared including inorganic salts.
Equipment: the test equipment include: erlenmeyer flasks, beakers, glass rod, pipetting tube, volumetric flasks ect.
Selection of test method: Manometric respirometry test was adopted.
Preparation of test substance stock solution: 52 mg test substance was dissolved in some test medium and then transferred to 500 ml volumetric flask. The stock solution was diluted with test medium to 500 ml and the stock solution with the effective nominal concentration of 100 mg/l was obtained (purity of the test substance is 96.5%)
Preparation of reference substance: 120 mg sodium benzoate was dissolved in test medium and then transferred to 100 ml volumetric flask. The stock solution was diluted with test medium to 100 ml and the stock solution with the concentration of 1200 mg/l was obtained.
Test groups include:
Bottles 1, 2 & A (test suspension): test substance, inoculum and test medium
Bottles 3, 4 & B (inoculum blank): inoculum and test medium
Bottle 5 (procedure control): reference substance, inoculum and test medium
Bottle 6: test substance, reference substance, inoculum and test medium
Preparation of test system:
addition of test substance: 100 ml test substance stock solution were added directly to the bottles 1, 2 and A respectively, 80 ml test substance stock solution was added to bottle 6.
addition of reference substance stock solution: 10 ml and 6.9 ml reference substance stock solution were added to bottles5 and 6 respectively.
addition of inoculum: 4.3 ml inoculum were added to bottles 1,2, A and 5; 5.0 ml inuculum were added to bottles 3,4 and B, 2.9 ml inoculum was added to bottle 6.
Addition of test medium: Test medium were added to bottles 1,2 A and 5 to make the final volume reached 365 ml; test medium were added to bottles 3, 4 and B to make the final volume reached 432 ml; Test medium was added to bottle 6 to make the final volume reached 250 ml.
Test substance effective concentration: 27 mg/l (bottles 1.2,A); 32 mg/l (bottle 6)
Reference substance concentration: 33 mg/l
inoculum concentration 28 mg/l
Test conditions: The test bottles were agitated and aerated on OxiTop control BOD at 22°C ± 1°C in the dark over 28 days period. - Reference substance:
- benzoic acid, sodium salt
- Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- >= 36.9 - <= 47.1
- Sampling time:
- 28 d
- Remarks on result:
- other: less than 60%
- Details on results:
- Temperature: 21.0°C -22.8°C
Percent biodegradation:
Reference substance (14d) 71.9%
Toxicity control (14 d): 51.3%
Test substance (14d): 0% (2 replicates)
Test substance (28 d): 36.9% 47.1% (2 replicates) 42% (average)
At the end of the test, the oxygen uptake of 2 inoculum blanks were 38.2 mg/l and 33.7 mg/l
At the end of the test, pH values of test suspensions were 7.24 and 7.21, those of inoculum blanks were 7.26 and 7.27 and pH values of the procedure control and toxicity control were 7.59 and 7.58.
At the start of the test NO2- N concentrations of the test suspension and inoculum blank were <0.10 mg/l and NO3-N concentrations were 2.27 mg/l and 2.25 mg/l respectively.
At the end of the test, NO2-N concentrations of the test suspension and inoculum blank were < 0.10 mg/l NO3-N concentrations of the test suspensions were 2.92 mg/l and 2.98 mg/l NO3-N concentrations of the inoculum blank were 1.65 mg/l and 1.87 mg/l. The results revealed that there was nitrification occured.
On the 14d of the test, the percentage biodegradation of the reference substance and toxicity control were 71.9 and 51.3, which showed that the inoculum activity could meet the requirement of the test and the test material was considered not to have a toxic effect on the sewage sludge micro-organisms used in the study.
Under the test conditions, the percantage biodegradation of the test substance were 0% and 0% on the 14d. The percentage biodegradation of the test substance were 36.9% and 47.1% on the 28 d, the average was 42%. - Validity criteria fulfilled:
- yes
- Interpretation of results:
- not readily biodegradable
- Conclusions:
- Under the test conditions, the percantage biodegradation of the test substance were 0% and 0% on the 14d. The percentage biodegradation of the test substance were 36.9% and 47.1% on the 28 d, the average was 42%.
The percentage biodegradation of the test substance did not reach 60% at the 10d window of the test. - Executive summary:
Ready biodegradation was tested on MEP using Manometric Respirometry test.
Test suspension, inoculum blank, procedure control, toxicity control were test groups used in the test. Inoculum was activated sludge.
The composition of oxygen of each test bottle was determined and recorded automatically by OxiTop contol BOD test system
Results:
Reference substance (14 d) 71.9%
Toxicity control (14d) 51.3%
Test substance (14d) 0% (two replicates)
Test substance (28d) 36.9% 47.1% (two replicates)
42% average
The percentage biodegradation of the test substance did not reach 60% at the 10d-window of the test under the conditions of the study presently performed.
Referenceopen allclose all
Description of key information
1. The test substance at a concentration of about 20 mg DOC per liter was incubated in an aerobic aqueous medium according to OECD guideline 301E.
The DOC removal markedly less than 5% was calculated for test substance group a over the whole examination period of 42 days. Calculating DOC removal for both substance groups combined, biodegradation did not exceed 9%. The substance is not biodegradable under test conditions. (1993 study)
2. Ready biodegradation was tested on MEP using Manometric Respirometry test.
Test suspension, inoculum blank, procedure control, toxicity control were test groups used in the test. Inoculum was activated sludge.
The composition of oxygen of each test bottle was determined and recorded automatically by OxiTop contol BOD test system
Results:
Reference substance (14 d) 71.9%
Toxicity control (14d) 51.3%
Test substance (14d) 0% (two replicates)
Test substance (28d) 36.9% 47.1% (two replicates)
42% average
The percentage biodegradation of the test substance did not reach 60% at the 10d-window of the test under the conditions of the study presently performed (2013 study).
Key value for chemical safety assessment
- Biodegradation in water:
- under test conditions no biodegradation observed
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.