1-ethyl-1-methylpyrrolidinium bromide

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

June-November 1993

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

yes

Details on sampling:

One liter of stock solution was prepared for each group (concentration) seperately by diluting the test substance solution with tap water. These stock solutions were then added each to 29 L water.

Vehicle:

no

Details on test solutions:

Dilution water (Local)
pH: ca. 8.5
Total hardness ca. 25 dH
Concentrations for the main test were derived from results of a preliminary test.
According to these results and to the OECD guideline, 5 concentrations were chosen between 1000 and 10000 mg/l test substance per liter water (1000, 1779, 3163, 5624, 10000) and control.

Test organisms (species):

Oncorhynchus mykiss (previous name: Salmo gairdneri)

Details on test organisms:

Rainbow trouts (Salmo gairdneri)
Source Fischuchtanstalt Kreuzstein, A-5310 Scharfling
Number 12 range finding test 60 main test
Body weight 0.98-1 gr (Mean groups A,B,K) 1.03-1.31 gr (Mean groups C, D, E)
Length 4.51- 4.62 cm (Mean groups A,B,K) 4.52-4.75 cm (Mean groups C, D, E)

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Hardness:

ca. 25 °dH

Test temperature:

means between 15.8 °C-15.9 °C over the test period

pH:

pH 8.41-8.51

Dissolved oxygen:

DOC for negative control : 91.7% and 103.1% of the air saturation value.
DOC of test material was 83% of the air saturation value immediately after preparation of the solution and 94% 5 days later.

Nominal and measured concentrations:

Mean actual concentrations of the test substances were between 100.4% and 102.0% of the nominal concentrations on Days 1 and 5 and have therefore also been satisfactory maintained over the test period. This layer chromatography of samples of the lowest and highest test substance concentration used (drawn on day 5 of the test) produced spots with the same rf-value as a sample of the original test substance solution supplied by the sponsor.
Concetration of the test substance in local untreated tap water were 1000, 1779, 3163, 5624, and 10000 mg/l.
10 fish were used for each concentration and for one negative control group.

Details on test conditions:

Water: dilution water, continuosly aerated by compressed air.
Holding: flow through
Exposure: up to 96 hr. cooling by stainless steel coolers inside the fish tanks.
Tank: Holding: glass tanks each containing ca. 200 L , covered with transparent plastic lids.
Exposure: full glass fish tanks, ca.47 cm x 29 cm x 31 cm, filled with 30 L of test media, covered with transparent plastic lids.
Loading:
Holding: 200 fish per tank maximum
Exposure: maximum of 0.5 g fish/1 test medium
Light: artifical light from 6 AM to 6 PM
Feed: EWOS- Lachsstarter-0/52-F, Raiffeisenverband Salzburg, Mischfutterwerk Saltzburg-Itzling, grinded.
Feeding: Holding: about 3 times per week, about 8 g per 100 fish
Exposure: no feeding (last feeding about 24 hr before start of the test)
Temperature, dissolved oxygen concentration and pH of the test media were determined before setting in the fish and about 24, 48, 72 and 96 hr after start of the test.
Acclimatization period: about 6 weeks
Mortality:
Holding: Following a 48-hr settling-in period, no fish died during consecutive 7 days. The batch was therefore accepted.

Reference substance (positive control):

not specified

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

5 600 mg/L

Nominal / measured:

meas. (initial)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

The concentration of the test substance was stable over the test period (greater than 99% of the actual starting concentration in all test media).
No mortality occured in the negative control group.
First responses of the fish to the test substance were observed about one hour after start of exposure. The most prominant finding was ataxia. First mortalities occured also about one hour after the start.
pH and temperature were stable throughout the test poriod.
Dissolved oxygen concentration was always greater than 91% of the saturation value of the negative control group.
The following results were achieved:

LC0/96 hr 1800mg/l MEP
LC100/96 hr > 10000 mg/l MEP
LC50/96 hr 5600 mg/l MEP

Validity criteria fulfilled:

yes

Conclusions:

The conditions for the validity of the test, according to the OECD guideline was fulfilled:
constant conditions were maintained (pH, Temperature), The concentration of the test substance was stable over the test period.
No mortality occured in the negative control group. First responses of the fish to the test substance were observed about one hour after start of exposure. The most prominent finding was ataxia. first mortalities occured also about hour after the start.
Results:
LC0/96hr: 1800 mg/l
LC100/96hr: >10000 mg/L
LC50/96hr: 5600 mg/L

Executive summary:

The conditions for the validity of the test, according to the OECD guideline was fulfilled

constant conditions were maintained (pH, Temperature),

The dissolved oxygen concentration was at least 60% of the air saturation value throughout the test in the negative control group. For the test substance groups, the detection method was inadequate , but there are strong indications that the DOC was also at least 60% of the air saturation value throughout the test period.

The concentration of the test substance has been satisfactorily maintained over the test period.

First responses of the fish to the test substance were observed about one hour after start of exposure, first mortalities occured also about one hour after the start. The max. concentration of MEP causing no mortality within the period of the test was 1779mg/l. The minimum concentration causing 100% motrality within the period of the test was not testedin this study (90% mortality at 10000mg test substance per liter of water).

in this static test with the test substance in rainbow trouts over a test period of 96 hr the LC50/96 hr was 5600 mg/l (95% confidence limits: 4300 and 7300 mg/l).

Description of key information

The conditions for the validity of the test, according to the OECD guideline was fulfilled:

constant conditions were maintained (pH, Temperature), The concentration of the test substance was stable over the test period.

No mortality occured in the negative control group. First  responses of the fish to the test substance were observed about one hour after start of exposure. The most prominent finding was ataxia. first mortalities occured also about hour after the start.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

5 600 mg/L