Isobutyl (S)-2-chloropropionate

Endpoint summary

Description of key information

oral 
LD50 rat: 1490 mg/kg bw (standardized protocol, comp. to OECD 401; BASF 1984) 
inhalation 
IHT rat, 7 h exposition to saturated vapour: no mortality after exposure to 8.1 mg/L nominal (standardized protocol, comp. to OECD 403; BASF 1986)

Additional information

There are reliable data from animal studies available to assess the acute oral and inhalative toxicity of the substance.

 

oral

In a study comparable to OECD test guideline 401 and following a standardized protocol (BASF test), 562, 1000, 1780 and 2610 mg/kg bw of the substance (purity unknown) were administered per gavage to five male and five female Wistar rats. The substance was prepared in an aqueous CMC solution and administered as 10 mL/kg bw volume dose. Animals were observed for 14 days before necropsy. The LD50 is 1490 mg/kg bw for both sexes (males 1650 mg/kg bw; females >1000 - < 1780 mg/kg bw). Totally, 1/10, 9/10, and 8/10 rats given 1000, 1780, and 2610 mg/kg bw, respectively, died. All deaths have to be considered as late deaths since animals died between day 2 and day 7 after application. Clinical signs that were observed in the two highest dose were dyspnea, apathy, staggering, atony, paresis, twitching, piloerection, imbalance and poor general state. Clinical signs were observed first at 3 days post dose and partially persisted until day 13 post dose. Accordingly, weight loss was observed in animals of the two highest doses in the first week of the observation period, but survivors recovered in the second week and gained weight. General congestive hyperemia and occasionally contentless stomach and gut was observed only in animals that died, while no pathological findings were observed in sacrificed animals (BASF 1984).

 

inhalation

In an inhalation hazard test according to the annex of OECD test guideline 403, three male and three female Wistar rats were whole-body exposed to a vapour saturated atmosphere of the test substance (purity unknown) for 7 h and at 20°C. The nominal concentration was 8.1 mg/L. Following a standard protocol, animals were observed for 14 days. Body weight and clinical symptoms were recorded. At the end of the observation period, both decedents and survivors were subjected to pathological examination. No deaths occurred after the treatment. During exposure, eyelid closure, serous secretion of eyes and nose (hemotest positive), salivation, and intermittent respiration was noted. After exposure, intermittent respiration and apathy was observed as reversible symptoms within one day. No necropsy findings were observed (BASF 1986).

Justification for classification or non-classification

According to the available test result, the test substance has to be classified as harmful if swallowed (R22 and acute oral Cat. 4 according to 67/548/EEC and GHS requirements, respectively).

According to the available test result, there is no indication given for a classification for the acute inhalative toxicity of the test substance according to 67/548/EEC and GHS requirements, respectively.