Methyl methanesulphonate

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Justification for type of information:

Data is from peer- reviewed journal

Data source

Reference

Reference Type:

publication

Title:

A method for determining the maximum tolerated dose for in vivo cytogenetic analysis

Author:

E. D. THOMPSON and R. A. HILES

Year:

1981

Bibliographic source:

Fd Cesmet. Toxicol. Vol.19, pp. 347 to 351. 1981.

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: as below

Principles of method if other than guideline:

Acute toxicity study of Methyl methanesulfonate in rat.

GLP compliance:

not specified

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

Methyl methanesulphonate

EC Number:

200-625-0

EC Name:

Methyl methanesulphonate

Cas Number:

66-27-3

Molecular formula:

C2H6O3S

IUPAC Name:

methyl methanesulfonate

Details on test material:

- Name of test material (as cited in study report): Methyl methanesulfonate (MMS)
- Molecular formula (if other than submission substance): C2H6O3S
- Molecular weight (if other than submission substance): 110.1324 g/mole
- Substance type: Organic
- Physical state: Solid
Purity No data available
- Impurities (identity and concentrations): No data available

Specific details on test material used for the study:

- Name of test material (as cited in study report): Methyl methanesulfonate (MMS)
- Molecular formula (if other than submission substance): C2H6O3S
- Molecular weight (if other than submission substance): 110.1324 g/mole
- Substance type: Organic
- Physical state: Solid

Test animals

Species:

rat

Strain:

Sprague-Dawley

Sex:

female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Weight at study initiation: 190-270 g
- Fasting period before study: Fasted overnight

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

water

Details on oral exposure:

VEHICLE
- Concentration in vehicle: 0, 0.15 and 0.21 g/kg
- Amount of vehicle (if gavage): 1 % solution in water
DOSAGE PREPARATION (if unusual): Methyl methanesulphonate was administered as a 1% solution in water.
CLASS METHOD (if applicable)
- Rationale for the selection of the starting dose: The estimated LD50 value being between the middle two doses.

Doses:

0, 0.15 and 0.21 g/kg (0, 150 and 210 mg/kg)

No. of animals per sex per dose:

Total: 30
0 g/kg: 10 female
0.15 g/kg: 10 female
0.21 g/kg: 10 female

Control animals:

yes

Details on study design:

No data

Statistics:

Slopes of the dose-response curves and the range of the doses were analyzed by Probit analyses.

Results and discussion

Preliminary study:

No data available

Effect levels

Sex:

female

Dose descriptor:

LD50

Effect level:

225 mg/kg bw

Based on:

test mat.

Remarks on result:

other: 50 % mortality observed

Mortality:

50% mortality was observed in treated rats at 225 mg/kg bw

Clinical signs:

other: No data available

Gross pathology:

No data available

Other findings:

No data

Any other information on results incl. tables

Results of acute LD50 studies in rats given methyl merhanesulphonare, glycidol or dimerhylsulphoxide

Compound	Oral administration
MMS†	Dose level (g/kg)	Lethal value*	LD50 (g/kg)
	0.15	4/10	0.225
	0.21	4/10

 

Applicant's summary and conclusion

Interpretation of results:

Category 3 based on GHS criteria

Conclusions:

LD50 was considered to be 225 mg/kg (0.225 g/kg) for Sprague-Dawley female rat when treated with Methyl methanesulfonate (MMS) orally.

Executive summary:

The acute oral toxicity using Methyl methanesulfonate (66-27-3) in 30 female Sprague-Dawley rats at the concentration of 0, 0.15 and 0.21 g/kg (0, 150 and 210 mg/kg). The given test chemical was dissolved as 1% solution in water and administered via oral gavage route.Animals were observed fir mortality.Slopes of the dose-response curves and the range of the doses were analyzed by probit analyses.50% mortality was observed in treated rats at 225 mg/kg bw. Therefore, LD50 was considered to be 225 mg/kg (0.225 g/kg), when Sprague-Dawley female rats were treated with Methyl methanesulfonate (MMS) via oral gavage route.