Sodium [3-hydroxy-4-[(1-hydroxy-5-sulpho-2-naphthyl)azo]naphthalene-1-sulphonato(4-)]chromate(1-)

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1982

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Version / remarks:

Pre-guideline test comparable to OECD 203 that was established later.

GLP compliance:

no

Specific details on test material used for the study:

None

Analytical monitoring:

no

Details on sampling:

Not available

Vehicle:

not specified

Details on test solutions:

No data

Test organisms (species):

no data

Details on test organisms:

TEST ORGANISM
- Common name: Zebra Fish

Test type:

not specified

Water media type:

not specified

Limit test:

no

Total exposure duration:

96 h

Post exposure observation period:

No data

Hardness:

No data

Test temperature:

25 °C

pH:

pH 6-8

Dissolved oxygen:

No data

Salinity:

No data

Nominal and measured concentrations:

300 and 1000 mg/L

Details on test conditions:

No data

Reference substance (positive control):

no

Duration:

96 h

Dose descriptor:

other: TL0

Effect conc.:

300 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

other: TL 50

Effect conc.:

608 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

other: TL100

Effect conc.:

1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

None

Results with reference substance (positive control):

None

Reported statistics and error estimates:

Not specified

Sublethal observations / clinical signs:

No mortality was seen at 300 mg/L concentration, however 9 out of 10 fish exposed to 1000 mg/L concentration were found dead at the end of observation period (96 h).

Validity criteria fulfilled:

not specified

Conclusions:

The 96h LC50 for FAT 20290/A is found to be 608 mg/L.

Executive summary:

Shot term toxicity of FAT 20290/A to Zebra fish was evaluated using methodology similar to OECD Guideline 203. In this study, two groups, each containing 10 fish, were exposed to test concentrations of 300 and 1000 mg/L for 96 hours. No mortality was seen at 300 mg/L concentration, however 9 out of 10 fish exposed to 1000 mg/L concentration were found dead at the end of observation period (96 h). Based on these findings, following results were obtained:

TL0 (96 h): 300 mg/L

TL50 (96 h): 608 mg/L

TL100 (96 h): 1000 mg/L

Description of key information

Short term toxicity of FAT 20290/A to Zebra fish was evaluated using a test protocol similar to OECD Guideline 203. In this study, two groups, each containing 10 fish, were exposed to test concentrations of 300 and 1000 mg/L for 96 hours. No mortality was seen at 300 mg/L concentration, however 9 out of 10 fish exposed to 1000 mg/L concentration were found dead at the end of observation period (96 h). Based on these findings, following results were obtained:

TL0 (96 h): 300 mg/L

TL50 (96 h): 608 mg/L

TL100 (96 h): 1000 mg/L

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

608 mg/L