Phosphonium, [(2E)-5-[(4R)-4-hydroxy-2,6,6-trimethyl-1-cyclohexen-1-yl]-3-methyl-2-penten-4-ynyl]triphenyl-, chloride

Skin irritation / corrosion

Administrative data

Endpoint:

skin irritation: in vivo

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: study performed according to OECD and GLP guidelines

Cross-reference

Cross-reference 1

Reason / purpose for cross-reference:

reference to same study

Cross-reference 2

Reason / purpose for cross-reference:

reference to other study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1997

Report date:

1997

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 404 (Acute Dermal Irritation / Corrosion)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

Zeanyne salt

EC Number:

618-777-5

Cas Number:

91662-51-0

Molecular formula:

C33H36ClOP

IUPAC Name:

Zeanyne salt

Test animals

Species:

rabbit

Strain:

New Zealand White

Test system

Type of coverage:

semiocclusive

Preparation of test site:

clipped

Vehicle:

water

Controls:

no

Duration of treatment / exposure:

4 h

Observation period:

14 d

Number of animals:

3

Results and discussion

In vivo

Results

Irritation parameter:

overall irritation score

Basis:

mean

Time point:

other: 7 d

Score:

0.67

Max. score:

8

Reversibility:

fully reversible within: 14 d

Other effects:

-

Any other information on results incl. tables

-

Applicant's summary and conclusion

Interpretation of results:

not irritating

Remarks:

Migrated information Criteria used for interpretation of results: EU

Conclusions:

Following evaluation of the experimental data according to the EEC classification criteria, Zeanyne is classified as
"not irritating" to rabbit skin.

Executive summary:

To assess the primary skin irritation potential of Zeanyne salt, a primary skin irritation

study was performed in three (one male and two females) young adult New Zealand White rabbits.

The fur of the left flank was clipped and 0.5 g of the substance was applied epicutaneously

to an area of approximately 6 cm2 and then covered with a semi-occlusive dressing for

approximately 4 hours. After removal of the dressing and test article, the skin reactions

(erythema and eschar, as well as oedema formation) were evaluated at 1, 24, 48 and 72 hours

after application. The primary irritation score was calculated to reflect and classify the irritant

potential of the test article.

The dermal exposure to Zeanyne salt caused very slight erythema and oedema in one

animal from one to 72 hours. Slight scaling of the skin was noted after 72 hours in this

animal. Well-defined erythema with marked scaling, scattered skin fissures (partly open and

bleeding) were recorded after 7 days. Only slightly shiny skin was noted after 14 days. No

findings were observed in the remaining two animals at any time during the study.

The Primary Irritation Score for this substance was 0.67 (the maximum attainable score is 8.0).

Following evaluation of the experimental data according to the EEC classification criteria, Zeanyne salt is classified as "not irritating" to

rabbit skin.