Prop-2-yn-1-ol

Eye irritation

Administrative data

Endpoint:

eye irritation: in vivo

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1963

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Study was performed prior to the implementation of GLP and OECD Guidelines, but is in general in compliance with the principles described in OECD Guideline 405.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1963

Report date:

1963

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 405 (Acute Eye Irritation / Corrosion)

Deviations:

not applicable

GLP compliance:

no

Remarks:

test predated GLP

Test material

Test material information

Constituent 1

Reference substance name:

Prop-2-yn-1-ol

EC Number:

203-471-2

EC Name:

Prop-2-yn-1-ol

Cas Number:

107-19-7

Molecular formula:

C3H4O

IUPAC Name:

prop-2-yn-1-ol

Details on test material:

No data

Test animals / tissue source

Species:

rabbit

Strain:

Vienna White

Details on test animals or tissues and environmental conditions:

no details given

Test system

Vehicle:

unchanged (no vehicle)

Controls:

not required

Amount / concentration applied:

TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 50 µl
- Concentration (if solution): undiluted

Duration of treatment / exposure:

continuous exposure, no wash-out after 24 hours

Observation period (in vivo):

The animals were observed several times on the treatment day and up to 8 days afterwards.

Number of animals or in vitro replicates:

2 animals

Details on study design:

REMOVAL OF TEST SUBSTANCE
- Washing: The eyes were not washed out after 24 hours.

SCORING SYSTEM: An internal scoring system was used (BASF grading for redness and chemosis of conjunctiva or corneal opacity).

TOOL USED TO ASSESS SCORE: Fluorescein staining of the cornea may have been performed at some time intervals in a study, however, these findings are not taken into account for the evaluation as this is not a standard requirement in the OECD test.

Results and discussion

In vivo

Results

Results 1

Irritation parameter:

conjunctivae score

Remarks:

(redness)

Basis:

mean

Remarks:

of 2 animals

Time point:

other: 1 and 24 hours

Score:

ca. 1.5

Max. score:

3

Reversibility:

not fully reversible within: 8 days

Remarks on result:

other: Redness grade 1 observed after 8 days

Results 2

Irritation parameter:

cornea opacity score

Basis:

mean

Remarks:

of 2 animals

Time point:

other: 1 and 24 hours

Score:

1

Max. score:

4

Reversibility:

not fully reversible within: 8 days

Remarks on result:

other: opacity grade 1 observed after 8 days

Results 3

Irritation parameter:

chemosis score

Basis:

mean

Remarks:

of 2 animals

Time point:

other: 1 and 24 hours

Score:

2

Max. score:

4

Reversibility:

not fully reversible within: 8 days

Remarks on result:

other: scar formation (upper lid) observed after 8 days

Irritant / corrosive response data:

The instillation of 50 µl propargyl alcohol into the conjunctival sac produced slight erythema and severe edema as well as slight corneal opacity after 1 hour. These changes did not subside during the first day, even after 8 days erythema and opacity remained and scarring of the upper lid was seen.

Applicant's summary and conclusion

Interpretation of results:

corrosive

Remarks:

Migrated information Criteria used for interpretation of results: EU

Conclusions:

In this BASF key study, undiluted Propargyl alcohol is corrosive to the rabbit eye.

Executive summary:

In a primary eye irritation study, 50 µl of undiluted Propargyl alcohol was instilled into the conjunctival sac of one eye of 2 White Vienna rabbits. The eyes were not washed out after 24 hours. Animals were observed for 8 days. After 1 hour, slight erythema and severe edema as well as slight corneal opacity were observed. These changes did not subside during the first day, even after 8 days erythema and opacity remained and scarring of the upper lid was seen. In this study, undiluted Propargyl alcohol is corrosive to the rabbit eye.